Catalog No.
YHJ96601
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Gly505
Predicted molecular weight
57.52 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9Y3I0
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
C22orf28, RNA-splicing ligase RtcB homolog, RTCB, 3'-phosphate/5'-hydroxy nucleic acid ligase
Regulation of plant gene expression by tsRNAs in response to abiotic stress., PMID:40421365
Structural and biochemical characterization of the 3'-5' tRNA splicing ligases., PMID:40220997
Mechanistic basis for PYROXD1-mediated protection of the human tRNA ligase complex against oxidative inactivation., PMID:40069351
Noncanonical circRNA biogenesis driven by alpha and gamma herpesviruses., PMID:40033018
DDX1 is required for non-spliceosomal splicing of tRNAs but not of XBP1 mRNA., PMID:39833356
Robust genome and cell engineering via in vitro and in situ circularized RNAs., PMID:39187662
Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease., PMID:38493148
RTCB deficiency triggers colitis in mice by influencing the NF-κB and Wnt/β-catenin signaling pathways., PMID:38425245
Fungi of the order Mucorales express a "sealing-only" tRNA ligase., PMID:38307611
Multiscale In Silico Study of the Mechanism of Activation of the RtcB Ligase by the PTP1B Phosphatase., PMID:38282538
Fungi of the order Mucorales express a "sealing-only" tRNA ligase., PMID:38014270
Insights into the structure and function of the RNA ligase RtcB., PMID:37935993
Thioredoxin regulates the redox state and the activity of the human tRNA ligase complex., PMID:37648453
The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1., PMID:37556111
Strain-Specific Gifsy-1 Prophage Genes Are Determinants for Expression of the RNA Repair Operon during the SOS Response in Salmonella enterica Serovar Typhimurium., PMID:36622230
Transcriptome Analysis of Acinetobacter baumannii in Rapid Response to Subinhibitory Concentration of Minocycline., PMID:36498165
The RNA repair proteins RtcAB regulate transcription activator RtcR via its CRISPR-associated Rossmann fold domain., PMID:36388977
High-throughput mapping of RNA solvent accessibility at the single-nucleotide resolution by RtcB ligation between a fixed 5'-OH-end linker and unique 3'-P-end fragments from hydroxyl radical cleavage., PMID:36369947
The RNA ligase RNA terminal phosphate cyclase B regulates mRNA alternative splicing and is required for mouse oocyte development and maintenance., PMID:36178098
Regulation of Archease by the mTOR-vATPase axis., PMID:36111596
Sequential rescue and repair of stalled and damaged ribosome by bacterial PrfH and RtcB., PMID:35858322
RtcB2-PrfH Operon Protects E. coli ATCC25922 Strain from Colicin E3 Toxin., PMID:35742896
Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs., PMID:35193953
Application of RtcB ligase to monitor self-cleaving ribozyme activity by RNA-seq., PMID:35025187
Identification and Functional Characterization of Divergent 3'-Phosphate tRNA Ligase From Entamoeba histolytica., PMID:34976851
cyPhyRNA-seq: a genome-scale RNA-seq method to detect active self-cleaving ribozymes by capturing RNAs with 2',3' cyclic phosphates and 5' hydroxyl ends., PMID:34906034
Molecular architecture of the human tRNA ligase complex., PMID:34854379
Putative RNA Ligase RtcB Affects the Switch between T6SS and T3SS in Pseudomonas aeruginosa., PMID:34830443
A 5' tRNA-Ala-derived small RNA regulates anti-fungal defense in plants., PMID:34705222
YY1 and RTCB in mouse uterine decidualization and embryo implantation., PMID:34591784
Protein Aggregation Patterns Inform about Breast Cancer Response to Antiestrogens and Reveal the RNA Ligase RTCB as Mediator of Acquired Tamoxifen Resistance., PMID:34206811
The oxidoreductase PYROXD1 uses NAD(P)+ as an antioxidant to sustain tRNA ligase activity in pre-tRNA splicing and unfolded protein response., PMID:33930333
The Transcriptomic Signature of Tigecycline in Acinetobacter baumannii., PMID:33193153
Ligation of 2', 3'-cyclic phosphate RNAs for the identification of microRNA binding sites., PMID:33113149
Soy Isoflavone Genistein Impedes Cancer Stemness and Mesenchymal Transition in Head and Neck Cancer through Activating miR-34a/RTCB Axis., PMID:32610494
Crystal structure of human archease, a key cofactor of tRNA splicing ligase complex., PMID:32234548
tRNA introns: Presence, processing, and purpose., PMID:31883233
RNA ligation precedes the retrotransposition of U6/LINE-1 chimeric RNA., PMID:31548405
Purification and enzymatic characterization of the RNA ligase RTCB from Thermus thermophilus., PMID:31280403
The bacterial RNA ligase RtcB accelerates the repair process of fragmented rRNA upon releasing the antibiotic stress., PMID:31250189
Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line., PMID:31045548
Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts., PMID:30962542
The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress., PMID:30355738
Genotoxic, Metabolic, and Oxidative Stresses Regulate the RNA Repair Operon of Salmonella enterica Serovar Typhimurium., PMID:30201777
Structural basis for the GTP specificity of the RNA kinase domain of fungal tRNA ligase., PMID:29165709
Site-Selective RNA Splicing Nanozyme: DNAzyme and RtcB Conjugates on a Gold Nanoparticle., PMID:29155548
Homology model of the human tRNA splicing ligase RtcB., PMID:28707320
RNA 3'-terminal phosphate cyclases and cyclase-like proteins., PMID:28132487
Functional analysis of the mammalian RNA ligase for IRE1 in the unfolded protein response., PMID:28093457
The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli., PMID:27789694
For research use only. Not for human or drug use.
