Catalog No.
YHF40101
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Lys183
Predicted molecular weight
23.21 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P61081
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M, UBE2M, UBC12, NEDD8-conjugating enzyme Ubc12
Neddylation modification stabilizes LC3B by antagonizing its ubiquitin-mediated degradation and promoting autophagy in skin., PMID:40208944
Pan-cancer analysis unveils the role and mechanisms of neddylation modifications in tumorigenesis., PMID:40106140
Blockade of neddylation through targeted inhibition of DCN1 alleviates renal fibrosis., PMID:39866066
Camk2n1 deficiency reduces the NaCl cotransporter activity through the CUL3/KLHL3/WNK4 complex in the kidney., PMID:39798916
Inhibition of the neddylation E2 enzyme UBE2M in macrophages protects against E. coli-induced sepsis., PMID:39675717
Jieduquyuziyin prescription alleviates lupus development via inhibiting neddylation pathway to promote Bim-induced apoptosis of double negative T cells., PMID:39362327
UBE2M forms a positive feedback loop with estrogen receptor to drive breast cancer progression and drug resistance., PMID:39138151
Biochemical and Structural Consequences of NEDD8 Acetylation., PMID:39022855
Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition., PMID:38329876
Proapoptotic effect of WS-299 induced by NOXA accumulation and NRF2-counterbalanced oxidative stress damage through targeting RBX1-UBE2M interaction in gastric cancers., PMID:38280358
Neddylation is a novel therapeutic target for lupus by regulating double negative T cell homeostasis., PMID:38221551
Identification of the communal pathogenesis and immune landscape between viral myocarditis and dilated cardiomyopathy., PMID:37967839
A CRISPR Screen of HIV Dependency Factors Reveals That CCNT1 Is Non-Essential in T Cells but Required for HIV-1 Reactivation from Latency., PMID:37766271
Discovery of neddylation E2s inhibitors with therapeutic activity., PMID:37717015
Neddylation-dependent LSD1 destabilization inhibits the stemness and chemoresistance of gastric cancer., PMID:37689288
Micafungin: A promising inhibitor of UBE2M in cancer cell growth suppression., PMID:37651876
The Functional Redundancy of Neddylation E2s and E3s in Modulating the Fitness of Regulatory T Cells., PMID:37600496
UBE2M-mediated neddylation of TRIM21 regulates obesity-induced inflammation and metabolic disorders., PMID:37343564
Pharmacologic targeting of Nedd8-activating enzyme reinvigorates T-cell responses in lymphoid neoplasia., PMID:37031300
A Virus-Packageable CRISPR System Identifies Host Dependency Factors Co-Opted by Multiple HIV-1 Strains., PMID:36744886
Neddylation-CRLs regulate the functions of Treg immune cells., PMID:36709423
NEDD8-conjugating enzyme E2s: critical targets for cancer therapy., PMID:36690633
Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner., PMID:36662617
Pseudogene UBE2MP1 derived transcript enhances in vitro cell proliferation and apoptosis resistance of hepatocellular carcinoma cells through miR-145-5p/RGS3 axis., PMID:36214767
Arctigenin impairs UBC12 enzyme activity and cullin neddylation to attenuate cancer cells., PMID:36138144
Dual role of neddylation in transcription of hepatitis B virus RNAs from cccDNA and production of viral surface antigen., PMID:36124123
Genome-wide analysis of genes encoding core components of the ubiquitin system during cerebral cortex development., PMID:35974412
Inhibition of NEDD8 NEDDylation induced apoptosis in acute myeloid leukemia cells via p53 signaling pathway., PMID:35880551
Integrative Analysis and Experimental Validation Indicated That SNHG17 Is a Prognostic Marker in Prostate Cancer and a Modulator of the Tumor Microenvironment via a Competitive Endogenous RNA Regulatory Network., PMID:35864871
The Ube2m-Rbx1 neddylation-Cullin-RING-Ligase proteins are essential for the maintenance of Regulatory T cell fitness., PMID:35641500
Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis., PMID:35403545
Combination of artesunate and WNT974 induces KRAS protein degradation by upregulating E3 ligase ANACP2 and β-TrCP in the ubiquitin-proteasome pathway., PMID:35305671
SKP2 drives the sensitivity to neddylation inhibitors and cisplatin in malignant pleural mesothelioma., PMID:35197103
Differential Gene Expression in Host Ubiquitination Processes in Childhood Malarial Anemia., PMID:34880904
Elevated Neddylation Pathway Promotes Th2 Cells Infiltration by Transactivating STAT5A in Hepatocellular Carcinoma., PMID:34804916
UBE2M Drives Hepatocellular Cancer Progression as a p53 Negative Regulator by Binding to MDM2 and Ribosomal Protein L11., PMID:34638383
Cullin 2-RBX1 E3 ligase and USP2 regulate antithrombin ubiquitination and stability., PMID:34324733
Inhibition of NAE-dependent protein hyper-NEDDylation in cystic cholangiocytes halts cystogenesis in experimental models of polycystic liver disease., PMID:34310849
Improvement of Oral Bioavailability of Pyrazolo-Pyridone Inhibitors of the Interaction of DCN1/2 and UBE2M., PMID:33945681
NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation., PMID:33905671
Targeting neddylation E2s: a novel therapeutic strategy in cancer., PMID:33827629
Development of phenyltriazole thiol-based derivatives as highly potent inhibitors of DCN1-UBC12 interaction., PMID:33756127
Neddylation pathway alleviates chronic pancreatitis by reducing HIF1α-CCL5-dependent macrophage infiltration., PMID:33723230
RNF111-facilitated neddylation potentiates cGAS-mediated antiviral innate immune response., PMID:33720974
MicroPOTS Analysis of Barrett's Esophageal Cell Line Models Identifies Proteomic Changes after Physiologic and Radiation Stress., PMID:33491460
Differential and spatial expression meta-analysis of genes identified in genome-wide association studies of depression., PMID:33414381
Molecular Network Profiling in Intestinal- and Diffuse-Type Gastric Cancer., PMID:33353109
Ubiquitin conjugating enzyme E2 M promotes apoptosis in osteoarthritis chondrocytes via Wnt/β-catenin signaling., PMID:32819607
NEDD8-conjugating enzyme UBC12 as a novel therapeutic target in esophageal squamous cell carcinoma., PMID:32651357
[Targeting Cullin-RING E3 ligases for anti-cancer therapy: efforts on drug discovery]., PMID:32621419
For research use only. Not for human or drug use.
