Catalog No.
KAV46601
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant HTNV Nucleoprotein has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-HTNV Nucleoprotein hIgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-HTNV Nucleoprotein hIgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Species reactivity
Hantaan virus (strain 76-118) (Korean hemorrhagic fever virus)
Applications
Used for the quantitative determination of Anti-HTNV Nucleoprotein hIgG concentration in serum and plasma.
Range
0.31 - 20 ng/mL
Sensitivity
0.28 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
For research use only. Not for human or drug use.
