Catalog No.
YXX08407
Description
NLS-Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 protein with a nucleic localization signal (NLS) on both N and C terminal, which can be used for genome editing by inducing site-specific DNA double stranded breaks. The Cas9 protein forms a highly stable ribonucleoprotein (RNP) complex with guide RNA (sgRNA). When equipped with the NLS sequence, the Cas9-RNP complex can promptly localize to the nucleus upon cell entry, eliminating the need for in vivo transcription or translation.
Expression system
E. coli
Species
Streptococcus pyogenes
Protein length
NLS-Cas9-NLS is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with a double-ends nuclear localization signal (NLS).
Predicted molecular weight
163 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Applications
1) Non-viral vector CRISPR gene knockout based on protein-sgRNA transfection. 2) Non-viral vector CRISPR gene knock-in based on protein transfection with sgRNA. 3) The DNA efficiency detection of sgRNA shearing target site reduces the cost of in vivo screening. 4) Specific sites of cleaved target DNA in vitro.
Form
Liquid
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Store at -20 ℃ for twelve months from the date of receipt.
Alternative Names
NLS-Cas9 Nuclease, Cas9, SpCas9, SpyCas9, CRISPR-associated endonuclease Cas9/Csn1
For research use only. Not for human or drug use.
