Catalog No.
YHE64501
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Glu21-Asp392
Predicted molecular weight
45.33 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P49336
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, Protein kinase K35, Cyclin-dependent kinase 8, CDK8, Cell division protein kinase 8
MED13L Pathogenic Missense Variants Impair Protein Stability and Interaction, Underlying Diverse Clinical Outcomes., PMID:40500968
A multi-omics exploration of PPARG activation in colon cancer: kinases featuring a PPRE sequence within regulatory regions., PMID:40500799
Identification of pyrazole scaffold inhibitors targeting cyclin-dependent kinase 8 for potential use in pulmonary fibrosis., PMID:40280247
The role of CDK8 gene polymorphisms in bladder cancer susceptibility and prognosis: a study in the Chinese Han population., PMID:40241036
UTERINE FIBROIDS., PMID:40214304
Knockout of cyclin-dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice., PMID:40172945
Cyclin-dependent kinases as mediators of aberrant transcription in prostate cancer., PMID:40163908
Targeting Mediator Kinase Cyclin-Dependent Kinases 8/19 Potentiates Chemotherapeutic Responses, Reverses Tumor Growth, and Prolongs Survival from Ovarian Clear Cell Carcinoma., PMID:40149277
Chemical composition and anticancer activity of Psychotria montana on MCF7 breast cancer cells: insights from in vitro (2D & 3D) studies and in silico analysis., PMID:40145882
Salt stress activates the CDK8-AHL10-SUVH2/9 module to dynamically regulate salt tolerance in Arabidopsis., PMID:40074748
A Protocol to Purify Human Mediator Complex From Freestyle 293-F Cells., PMID:40028032
An Effective Reaction-Based Virtual Screening Method to Discover New CDK8 Ligands., PMID:39972506
Association of CDK8 Gene Polymorphisms with Cervical Cancer in Han Women in Southwest China., PMID:39964205
Structural optimization and biological evaluation of indolin-2-one derivatives as novel CDK8 inhibitors for idiopathic pulmonary fibrosis., PMID:39955852
Mediator kinase inhibition suppresses hyperactive interferon signaling in Down syndrome., PMID:39928031
Mediator kinase inhibition drives myometrial stem cell differentiation and the uterine fibroid phenotype through super-enhancer reprogramming., PMID:39904883
Short IL-18 generated by caspase-3 cleavage mobilizes NK cells to suppress tumor growth., PMID:39891018
The Cyclin-Dependent Kinase 8 Inhibitor E966-0530-45418 Attenuates Pulmonary Fibrosis In Vitro and In Vivo., PMID:39781457
Phenotype-Led Identification of IL-10 Upregulators in Human CD4+ T-cells and Elucidation of Their Pharmacology as Highly Selective CDK8/CDK19 Inhibitors., PMID:39780505
Transcriptional coupling of telomeric retrotransposons with the cell cycle., PMID:39752503
Identification of a Potent CDK8 Inhibitor Using Structure-Based Virtual Screening., PMID:39740163
Subunits Med12 and Med13 of Mediator Cooperate with Subunits SAYP and Bap170 of SWI/SNF in Active Transcription in Drosophila., PMID:39684492
CDK8 inhibitor KY-065 rescues skeletal abnormalities in achondroplasia model mice., PMID:39674288
Transcriptional Regulation of Protein Synthesis by Mediator Kinase Represents a Therapeutic Vulnerability in MYC-driven Medulloblastoma., PMID:39574899
The CDK8 kinase module: A novel player in the transcription of translation initiation and ribosomal genes., PMID:39565680
Mediator kinase inhibitors suppress triple-negative breast cancer growth and extend tumor suppression by mTOR and AKT inhibitors., PMID:39541354
Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila., PMID:39531377
Myeloid Mir34a suppresses colitis-associated colon cancer: characterization of mediators by single-cell RNA sequencing., PMID:39425000
CDK8/19 inhibition triggers a switch from mitosis to endomitosis in cord blood megakaryocytes., PMID:39413769
Cdk8 and Hira mutations trigger X chromosome elimination in naive female hybrid mouse embryonic stem cells., PMID:39390295
Synthesis and Evaluation of a Novel Series of Diphenylamine and Diphenylether Derivatives with Osteoblastogenic and Osteogenic Effects via CDK8 Inhibition., PMID:39370261
Cyclin-Dependent Kinase 8 Represents a Positive Regulator of Cytomegalovirus Replication and a Novel Host Target for Antiviral Strategies., PMID:39339274
Structural basis of the human transcriptional Mediator regulated by its dissociable kinase module., PMID:39321804
Med13 is required for efficient P-body recruitment and autophagic degradation of Edc3 following nitrogen starvation., PMID:39320938
Discovery of flavonoid-containing compound Lupalbigenin as anti-NSCLC cancer agents via suppression of EGFR and ERK1/2 pathway., PMID:39288634
MED12 and CDK8/19 Modulate Androgen Receptor Activity and Enzalutamide Response in Prostate Cancer., PMID:39253786
An intrinsically disordered region in MED13 turns Mediator on/off on cue., PMID:39178836
CDK8 of the mediator kinase module connects leaf development to the establishment of correct stomata patterning by regulating the levels of the transcription factor SPEECHLESS (SPCH)., PMID:39177450
Enriching Anticancer Drug Pipeline with Potential Inhibitors of Cyclin-Dependent Kinase-8 Identified from Natural Products., PMID:39149808
CDK8/19 inhibitor enhances arginase-1 expression in macrophages via STAT6 and p38 MAPK activation., PMID:39067565
Structures and compositional dynamics of Mediator in transcription regulation., PMID:39067114
Mechanisms of heat stress-induced transcriptional memory., PMID:38968911
An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II., PMID:38955181
IKZF1 gene deletions drive resistance to cytarabine in B-cell precursor acute lymphoblastic leukemia., PMID:38841778
Bromo-substituted indirubins for inhibition of protein kinase-mediated signalling involved in inflammatory mediator release in human monocytes., PMID:38838619
Highlighting the Major Role of Cyclin C in Cyclin-Dependent Kinase 8 Activity through Molecular Dynamics Simulations., PMID:38791449
Distinct effects of CDK8 module subunits on cellular growth and proliferation in Drosophila., PMID:38746212
An ensemble machine learning model generates a focused screening library for the identification of CDK8 inhibitors., PMID:38723187
The discovery of a novel pyrrolo[2,3-b]pyridine as a selective CDK8 inhibitor offers a new approach against psoriasis., PMID:38713949
Discovery of a Novel and Potent Cyclin-Dependent Kinase 8/19 (CDK8/19) Inhibitor for the Treatment of Cancer., PMID:38690856
For research use only. Not for human or drug use.
