Catalog No.
KAV46402
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant CHIKV NSP1 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-CHIKV NSP1 hIgM present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgM antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-CHIKV NSP1 hIgM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Applications
Used for the quantitative determination of Anti-CHIKV NSP1 hIgM concentration in Human serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
156.25 - 10,000 ng/mL
Sensitivity
102.56 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
4339.3 |
1087.5 |
732.1 |
4101.1 |
1038.0 |
519.3 |
|
Standard deviation |
313.6 |
101.8 |
69.8 |
258.5 |
98.6 |
76.4 |
|
CV (%) |
7.2 |
9.4 |
9.5 |
6.3 |
9.5 |
14.7 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Alternative Names
Anti-CHIKV NSP1 hIgM, CHIKV, NSP1, NS1, hIgM, Chikungunya virus
For research use only. Not for human or drug use.
