Anti-LC3B Antibody (R3W32)

Catalog No.

RHJ53202

Species reactivity

Human

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

IHC: 1:50-1:100

Target

MAP1A/MAP1B light chain 3 B, MAP1 light chain 3-like protein 2, MAP1ALC3, MAP1LC3B, Autophagy-related protein LC3 B, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A/MAP1B LC3 B

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9GZQ8

Applications

IHC

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.5% BSA, 0.05% Sodium Azide and 50% Glycerol.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3W32

Hydroxychloroquine confers placental protection in antiphospholipid Syndrome: Mechanistic insights into autophagy regulation and trophoblast dysfunction., PMID:40393103

Traumatic brain injury and autophagy: a pilot study about the immunohistochemical expression of LC3B, Beclin 1, p62, and LAMP2A in human autoptic samples., PMID:40356869

NLRP3 inflammasome inhibits mitophagy during the progression of temporal lobe epilepsy., PMID:40348802

Synergistic Induction of Immunogenic Cell Death by Biomineralized Manganese and Bisphosphonates Enhances Anti-PD-L1 Therapy in Triple-Negative Breast Cancer., PMID:40276640

IMGN853 Induces Autophagic Cell Death in Combination Therapy for Ovarian Cancer., PMID:40029935

HMGB1 induces unexplained recurrent spontaneous abortion by mediating decidual macrophage autophagy., PMID:39787761

Cervical Cancer Cells Use the CD95 and IL-2 Pathways to Promote Their Proliferation and Survival., PMID:39766250

CKAP4 in hepatocellular carcinoma: competitive RETREG1/FAM134B binding, reticulophagy regulation, and cancer progression., PMID:39689859

Avian TRIM13 attenuates antiviral innate immunity by targeting MAVS for autophagic degradation., PMID:39508267

Hyperbaric Treatment Stimulates Chaperone-Mediated Macroautophagy and Autophagy in the Liver Cells of Healthy Female Rats., PMID:39408803

Zn0-Induced Cytotoxicity and Mitochondrial Stress in Microglia: Implications of the Protective Role of Immunoglobulin G In Vitro., PMID:39129512

Immunoexpression Pattern of Autophagy-Related Proteins in Human Congenital Anomalies of the Kidney and Urinary Tract., PMID:38999938

Anti-IL-8 monoclonal antibodies inhibits the autophagic activity and cancer stem cells maintenance within breast cancer tumor microenvironment., PMID:38552109

Immunohistochemical Expression of Autophagy-Related Marker (LC3B) and Stem Cell Marker (CD44) in Molecular Subtypes of Breast Cancer., PMID:38285778

Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair., PMID:37978868

HMGB1-RAGE axis contributes to myocardial ischemia/reperfusion injury via regulation of cardiomyocyte autophagy and apoptosis in diabetic mice., PMID:37768929

[Effect of acupotomy on mitophagy mediated by PINK1/Parkin pathway in cartilage of rabbits with knee osteoarthritis]., PMID:37730260

Tumor-derived lactate promotes resistance to bevacizumab treatment by facilitating autophagy enhancer protein RUBCNL expression through histone H3 lysine 18 lactylation (H3K18la) in colorectal cancer., PMID:37615625

[Autophagy Markers Induced by Influenza Virus and MUC1 Expressions in Cancer-Derived Cell Lines]., PMID:37462303

Enhancement of SARS-CoV-2 N Antigen-Specific T Cell Functionality by Modulating the Autophagy-Mediated Signal Pathway in Mice., PMID:37376617

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures., PMID:37311031

EPOR activation attenuates colitis via enhancing LC3B-dependent apoptotic cell clearance., PMID:37204242

Autophagy-related genes analysis reveals potential biomarkers for prediction of the impaired walking capacity of peripheral arterial disease., PMID:37198605

Immunoexpression Pattern of Autophagy Markers in Developing and Postnatal Kidneys of Dab1-/-(yotari) Mice., PMID:36979337

Evaluation of the mTORC activity in the presence of Toxoplasma gondii and azathioprine in human monocyte cell line., PMID:36941573

Resveratrol Protects against Zearalenone-Induced Mitochondrial Defects during Porcine Oocyte Maturation via PINK1/Parkin-Mediated Mitophagy., PMID:36136579

Cell Cycle Analysis of ER Stress and Autophagy., PMID:36087266

Salvianolic acid B inhibits autophagy and activation of hepatic stellate cells induced by TGF-β1 by downregulating the MAPK pathway., PMID:35991894

Lens Aquaporin-5 Inserts Into Bovine Fiber Cell Plasma Membranes Via Unconventional Protein Secretion., PMID:35816045

Three-Dimensional Microfluidic Chip for Efficient Capture of Secretory Autophagosomes and Sensitive Detection of Their Surface Proteins., PMID:35657105

Tubular cells produce FGF2 via autophagy after acute kidney injury leading to fibroblast activation and renal fibrosis., PMID:35491858

Vitamin D Attenuates Airway Inflammation in Asthmatic Guinea Pigs Using Mammalian Target of Rapamycin-Mediated Autophagy., PMID:35438528

DNA damage and repair in the visual center in the rhesus monkey model of glaucoma., PMID:35364099

Expression of Autophagy Markers Beclin1 and LC3B in Prostatic Carcinoma: An Immunohistochemical Case-Control Study., PMID:35096092

The multitargeted receptor tyrosine kinase inhibitor sunitinib induces resistance of HER2 positive breast cancer cells to trastuzumab-mediated ADCC., PMID:35066605

Belimumab Decreases Autophagy and Citrullination in Peripheral Blood Mononuclear Cells from Patients with Systemic Lupus Erythematosus., PMID:35053379

Etanercept Mitigates Cadmium Chloride-induced Testicular Damage in Rats "An Insight into Autophagy, Apoptosis, Oxidative Stress and Inflammation"., PMID:34993805

Mechanisms in Graves Eye Disease: Apoptosis as the End Point of Insulin-Like Growth Factor 1 Receptor Inhibition., PMID:34927457

Rescue of thyroid cells from antibody induced cell death via induction of autophagy., PMID:34801870

Mucin 1 as a Molecular Target of a Novel Diisoquinoline Derivative Combined with Anti-MUC1 Antibody in AGS Gastric Cancer Cells., PMID:34770912

Extracellular vesicles from Fasciola gigantica induce cellular response to stress of host cells., PMID:34742714

Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture., PMID:34740307

Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression., PMID:34668631

Selenium inhibits growth of trastuzumab-resistant human breast cancer cells via downregulation of Akt and beclin-1., PMID:34525121

OxLDL as an Inducer of a Metabolic Shift in Cancer Cells., PMID:34475995

Interaction of Poliovirus Capsid Proteins with the Cellular Autophagy Pathway., PMID:34452452

Anti-HER2 monoclonal antibodies intensify the susceptibility of human gastric cancer cells to etoposide by promoting apoptosis, but not autophagy., PMID:34437575

Silver birch pollen-derived microRNAs promote NF-κB-mediated inflammation in human lung cells., PMID:34392209

LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells., PMID:34313548

Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter., PMID:34311870

Document Download

Anti-LC3B Antibody (R3W32).pdf