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Anti-TUBB3/Beta Tubulin Antibody (R3S60)

Catalog #:   RHG56705 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IHC, WB
Accession: Q13509
Overview

Catalog No.

RHG56705

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000

Target

Tubulin beta-III, TUBB4, TUBB3, Tubulin beta-4 chain, Tubulin beta-3 chain

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q13509

Applications

ELISA, FCM, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3S60

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using TUBB3 mouse mAb (green) and negative control (red).
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using TUBB3 mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using TUBB3 mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using TUBB3 mouse mAb against NIH/3T3 (1), Hela (2), and A549 (3) cell lysate.
References

High Intraocular Concentration of Fibrinogen Regulates Retinal Function Via the ICAM-1 Pathway., PMID:39546292

Single-extracellular vesicle (EV) analyses validate the use of L1 Cell Adhesion Molecule (L1CAM) as a reliable biomarker of neuron-derived EVs., PMID:38868956

Regenerated Corneal Epithelium Expresses More βIII-Tubulin After Chemical Injuries Compared to Mechanical Injuries., PMID:38085248

Randomised phase II trial of weekly ixabepilone ± biweekly bevacizumab for platinum-resistant or refractory ovarian/fallopian tube/primary peritoneal cancer., PMID:35149854

Nuclear IMPDH Filaments in Human Gliomas., PMID:34498062

Modulation of the IGF1R-MTOR pathway attenuates motor neuron toxicity of human ALS SOD1G93A astrocytes., PMID:33749521

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

BET inhibition increases βIII-tubulin expression and sensitizes metastatic breast cancer in the brain to vinorelbine., PMID:32848091

Characterization of T-DM1-resistant breast cancer cells., PMID:32583565

Alpha- and beta-tubulin isotypes are differentially expressed during brain development., PMID:32293117

Monoclonal antibody AC10364 inhibits cell proliferation in 5-fluorouracil resistant hepatocellular carcinoma via apoptotic pathways., PMID:31303763

An in vitro developmental neurotoxicity screening assay for retinoic acid-induced neuronal differentiation using the human NT2/D1 cell line., PMID:30980846

Optic Nerve Head Astrocytes Display Axon-Dependent and -Independent Reactivity in Response to Acutely Elevated Intraocular Pressure., PMID:30665231

Preoperative Anti-Class III β-Tubulin Antibodies As Relevant Clinical Biomarkers in Ovarian Cancer., PMID:29448203

High protein and mRNA expression levels of TUBB3 (class III ß-tubulin) are associated with aggressive tumor features in esophageal adenocarcinomas., PMID:29383151

Post-Translational Tubulin Modifications in Human Astrocyte Cultures., PMID:28512712

Comparison of the Effects of Curcumin and RG108 on NGF-Induced PC-12 Adh Cell Differentiation and Neurite Outgrowth., PMID:28338387

Structure of neuro-endocrine and neuro-epithelial interactions in human foetal pancreas., PMID:27823763

Malignant glioma with primitive neuroectodermal tumor-like component (MG-PNET): novel microarray findings in a pediatric patient., PMID:27781423

Corneal Reinnervation and Sensation Recovery in Patients With Herpes Zoster Ophthalmicus: An In Vivo and Ex Vivo Study of Corneal Nerves., PMID:26989956

Induction of neural differentiation in rat C6 glioma cells with taxol., PMID:26665000

Human organotypic retinal cultures (HORCs) as a chronic experimental model for investigation of retinal ganglion cell degeneration., PMID:26432917

Coordinated interaction of Down syndrome cell adhesion molecule and deleted in colorectal cancer with dynamic TUBB3 mediates Netrin-1-induced axon branching., PMID:25754961

An agarose gel-based neurosphere culture system leads to enrichment of neuronal lineage cells in vitro., PMID:25539864

Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts., PMID:25343338

Tubulin β-III: a novel immunohistochemical marker for intrahepatic peripheral cholangiocarcinoma., PMID:25039376

Effect of all-trans retinoic acid on the differentiation of U87 glioma stem/progenitor cells., PMID:23852377

Autoantibodies against neuronal progenitors in sera from children with autism., PMID:23838310

Human mesenchymal stem cells express neuronal markers after osteogenic and adipogenic differentiation., PMID:23430457

Blockade of VEGFR-1 and VEGFR-2 enhances paclitaxel sensitivity in gastric cancer cells., PMID:23364970

[Immunohistochemical characteristics of human brain vascular plexuses]., PMID:23342655

Use of high content image analyses to detect chemical-mediated effects on neurite sub-populations in primary rat cortical neurons., PMID:23142577

An angiogenesis model for investigating multicellular interactions across intact microvascular networks., PMID:23125212

Overexpression of class III beta tubulin and amplified HER2 gene predict good response to paclitaxel and trastuzumab therapy., PMID:23028798

Myelin sheath decompaction, axon swelling, and functional loss during chronic secondary degeneration in rat optic nerve., PMID:22879411

[Molecular cloning of tubulin beta 3 (TUBB3) in Gekko japonicus and preparation of its polyclonal antibody]., PMID:22855447

Limiting multiple sclerosis related axonopathy by blocking Nogo receptor and CRMP-2 phosphorylation., PMID:22544872

Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia., PMID:22134676

Identification of class III β-tubulin as a marker of angiogenic perivascular cells., PMID:21958528

Disease-free survival of patients after surgical resection of non-small cell lung carcinoma and correlation with excision repair cross-complementation group 1 expression and genotype., PMID:21899657

Neural stem/progenitor cells circulating in peripheral blood of patients with neovascular form of AMD: a novel view on pathophysiology., PMID:21847578

Optineurin inclusions occur in a minority of TDP-43 positive ALS and FTLD-TDP cases and are rarely observed in other neurodegenerative disorders., PMID:21360076

Oral soft tissue alterations in patients with neurofibromatosis., PMID:21301902

Neutralization of BDNF attenuates the in vitro protective effects of olfactory ensheathing cell-conditioned medium on scratch-insulted retinal ganglion cells., PMID:21082236

Immunoreactivity for high-affinity choline transporter colocalises with VAChT in human enteric nervous system., PMID:20490865

Expression of tubulin, p53, ki67, receptors for estrogen, and progesterone in endometrial cancer., PMID:19899405

Detection of pain-related molecules in the subchondral bone of osteoarthritic knees., PMID:19730932

Brain-derived neurotrophic factor released from engineered mesenchymal stem cells attenuates glutamate- and hydrogen peroxide-mediated death of staurosporine-differentiated RGC-5 cells., PMID:19524566

Secondary retinal ganglion cell death and the neuroprotective effects of the calcium channel blocker lomerizine., PMID:19474405

[Merkel cell carcinoma--immunohistochemical study in a group of 11 patients]., PMID:19402315

Datasheet
$ 220
Product specifications
50 μg 220 100 μg 350

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For research use only. Not for human or drug use.

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Anti-TUBB3/Beta Tubulin Antibody (R3S60) [RHG56705]
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