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Anti-VCP Antibody (R3N34)

Catalog #:   RHF08802 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: ELISA, FCM, IF, WB
Accession: P55072
Overview

Catalog No.

RHF08802

Species reactivity

Human, Mouse

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Target

Transitional endoplasmic reticulum ATPase, Valosin-containing protein, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, VCP

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P55072

Applications

ELISA, FCM, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R3N34

Data Image
  • Flow Cytometry
    Flow cytometric analysis of MOLT4 cells using VCP mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of HL-60 cells using VCP mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of THP-1 cells using VCP mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using VCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Western blot
    Western blot analysis using VCP mouse mAb against Hela (1), A549 (2), NIH/3T3 (3), Raw264.7 (4), SH-SY5Y (5), MCF-7 (6), and A431 (7) cell lysate.
  • Western blot
    Western blot analysis using VCP mouse mAb against Hela (1), A549 (2), NIH/3T3 (3), Raw264.7 (4), SH-SY5Y (5), MCF-7 (6), and A431 (7) cell lysate.
References

Myasthenia Gravis Presenting With Vocal Cord Paralysis As the Initial Symptom Occurring One Week Following Herpes Zoster Vaccination: A Case Report and Review of the Literature., PMID:40462824

Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers., PMID:40291716

Identification of biomarkers of shrinkage modes after neoadjuvant therapy in HER-2 positive breast cancer., PMID:40143733

Molecular identification of a thioredoxin peroxidase in Babesia gibsoni with potential against oxidative stress., PMID:40045010

A universal viral capsid protein based one step RNA synthesis and packaging system for rapid and efficient mRNA vaccine development., PMID:40022448

VCP/p97-associated proteins are binders and debranching enzymes of K48-K63-branched ubiquitin chains., PMID:38977901

Fecal virus transplantation has more moderate effect than fecal microbiota transplantation on changing gut microbial structure in broiler chickens., PMID:38147728

TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing., PMID:37816088

An arrayed CRISPR screen of primary B cells reveals the essential elements of the antibody secretion pathway., PMID:36860866

Anti-Valosin-Containing Protein (VCP/p97) Autoantibodies in Inclusion Body Myositis and Other Inflammatory Myopathies., PMID:36373433

Synthetic antibody-derived immunopeptide provides neuroprotection in glaucoma through molecular interaction with retinal protein histone H3.1., PMID:36313990

Adaptor-Specific Antibody Fragment Inhibitors for the Intracellular Modulation of p97 (VCP) Protein-Protein Interactions., PMID:35819848

Safety and immunogenicity of four-segmented Rift Valley fever virus in the common marmoset., PMID:35585071

p97 dysfunction underlies a loss of quality control of damaged membrane proteins and promotes oxidative stress and sickling in sickle cell disease., PMID:35405035

Neurons with Cat's Eyes: A Synthetic Strain of α-Synuclein Fibrils Seeding Neuronal Intranuclear Inclusions., PMID:35327628

Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy., PMID:34572103

Dermatan Sulfate Is a Potential Regulator of IgH via Interactions With Pre-BCR, GTF2I, and BiP ER Complex in Pre-B Lymphoblasts., PMID:34113352

Multiple myeloma cells are exceptionally sensitive to heat shock, which overwhelms their proteostasis network and induces apoptosis., PMID:32817432

Preparation and qualification of internal rabies reference standards for use in the rabies rapid fluorescent focus inhibition test., PMID:32555434

Phospho-Ser784-VCP Is Required for DNA Damage Response and Is Associated with Poor Prognosis of Chemotherapy-Treated Breast Cancer., PMID:32521270

Development of a therapeutic anti-HtrA1 antibody and the identification of DKK3 as a pharmacodynamic biomarker in geographic atrophy., PMID:32345717

Nonlinear pharmacokinetics of rituximab in non-Hodgkin lymphomas: A pilot study., PMID:31112622

Vocal cord paralysis as primary and secondary results of malignancy. A prospective descriptive study., PMID:31024994

[Successful treatment with preceding low-intensity chemotherapy in a primary mediastinal large B cell lymphoma patient diagnosed at 11 weeks of pregnancy]., PMID:30842377

Upregulation of valosin-containing protein (VCP) is associated with poor prognosis and promotes tumor progression of orbital B-cell lymphoma., PMID:30643422

Magnetic Focus Lateral Flow Sensor for Detection of Cervical Cancer Biomarkers., PMID:30632735

A novel probe to assess cytosolic entry of exogenous proteins., PMID:30082832

Species Differences in the Organization of the Ventral Cochlear Nucleus., PMID:29236365

Cytosolic Fc receptor TRIM21 inhibits seeded tau aggregation., PMID:28049840

Caveolin-1 is an aggresome-inducing protein., PMID:27929047

Autophagy and ubiquitin-proteasome system contribute to sperm mitophagy after mammalian fertilization., PMID:27551072

Antibodies directed against endogenous and exogenous citrullinated antigens pre-date the onset of rheumatoid arthritis., PMID:27255888

Silencing of Small Valosin-containing Protein-interacting Protein (SVIP) Reduces Very Low Density Lipoprotein (VLDL) Secretion from Rat Hepatocytes by Disrupting Its Endoplasmic Reticulum (ER)-to-Golgi Trafficking., PMID:27129256

The Myoblast C2C12 Transfected with Mutant Valosin-Containing Protein Exhibits Delayed Stress Granule Resolution on Oxidative Stress., PMID:27106764

Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody., PMID:26865113

Lysine Methylation of the Valosin-Containing Protein (VCP) Is Dispensable for Development and Survival of Mice., PMID:26544960

Genome-Wide Screen Reveals Valosin-Containing Protein Requirement for Coronavirus Exit from Endosomes., PMID:26311884

CHMP5 controls bone turnover rates by dampening NF-κB activity in osteoclasts., PMID:26195726

Effects of acoustic trauma on the auditory system of the rat: The role of microglia., PMID:26162240

Regulation of VCP/p97 demonstrates the critical balance between cell death and epithelial-mesenchymal transition (EMT) downstream of ER stress., PMID:25970786

Randomized trial of vaccination in fingolimod-treated patients with multiple sclerosis., PMID:25636714

Levels of metacaspase1 and chaperones related to protein quality control in alcoholic and nonalcoholic steatohepatitis., PMID:25526666

Integrative quantitative proteomics unveils proteostasis imbalance in human hepatocellular carcinoma developed on nonfibrotic livers., PMID:25225353

Waldenström macroglobulinaemia and intestinal lymphangiectasia., PMID:25039993

ATP7A trafficking and mechanisms underlying the distal motor neuropathy induced by mutations in ATP7A., PMID:24754450

Expression level of valosin containing protein is associated with prognosis of primary orbital MALT lymphoma., PMID:24377547

Characterization, expression and localization of valosin-containing protein in ovaries of the giant tiger shrimp Penaeus monodon., PMID:24095778

Simultaneous neutralization and innate immune detection of a replicating virus by TRIM21., PMID:23596308

AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization., PMID:23091005

A systems biology approach to identify the signalling network regulated by Rho-GDI-γ during neural stem cell differentiation., PMID:22892720

Datasheet

Document Download

Anti-VCP Antibody (R3N34).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-VCP Antibody (R3N34) [RHF08802]
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