Catalog No.
RHE29802
Species reactivity
Human
Host species
Mouse
Isotype
IgG1
Clonality
Monoclonal
Tested applications
ELISA: 1:10000, WB: 1:500-1:2000
Target
Aquaporin-2, AQP-CD, AQP2, Water channel protein for renal collecting duct, AQP-2, WCH-CD, Collecting duct water channel protein, Aquaporin-CD, ADH water channel
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P41181
Applications
ELISA, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
R3K49
Focal Adhesion Kinase (FAK) inhibition induces membrane accumulation of aquaporin2 (AQP2) through endocytosis inhibition and actin depolymerization in renal epithelial cells., PMID:39416213
Optimizing renal transporter immunodetection: consequences of freeze-thaw during sample preparation., PMID:39205660
Cannabinoid receptor type 1 activation causes a water diuresis by inducing an acute central diabetes insipidus in mice., PMID:38634131
Collecting duct water permeability inhibition by EGF is associated with decreased cAMP, PKA activity, and AQP2 phosphorylation at Ser269., PMID:38205543
Structural analysis of the water channel AQP2 by single-particle cryo-EM., PMID:37315821
Novel concept of Wilms' tumor development: involvement of pluripotential cells of ureteric bud., PMID:37209922
Identification of protein kinase A signalling molecules in renal collecting ducts., PMID:37013848
Intracellular sites of AQP2 S256 phosphorylation identified using inhibitors of the AQP2 recycling itinerary., PMID:36454701
Urinary reabsorption in the rat kidney by anticholinergics., PMID:33911165
Phosphorylation profile of human AQP2 in urinary exosomes by LC-MS/MS phosphoproteomic analysis., PMID:32529500
Uterine and placental specific localization of AQP2 and AQP8 is related with changes of serum progesterone levels in pregnant queens., PMID:31593882
Site-1 protease-derived soluble (pro)renin receptor targets vasopressin receptor 2 to enhance urine concentrating capability., PMID:30944256
Genome-Wide Mapping of DNA Accessibility and Binding Sites for CREB and C/EBPβ in Vasopressin-Sensitive Collecting Duct Cells., PMID:29572403
AQP2 in human urine is predominantly localized to exosomes with preserved water channel activities., PMID:29396622
Ezrin directly interacts with AQP2 and promotes its endocytosis., PMID:28754689
Effects of gonadectomy and testosterone treatment on aquaporin expression in the kidney of normotensive and hypertensive rats., PMID:28399644
Targeting gene expression to specific cells of kidney tubules in vivo, using adenoviral promoter fragments., PMID:28253301
Hypercalcemia induces targeted autophagic degradation of aquaporin-2 at the onset of nephrogenic diabetes insipidus., PMID:28139295
Disruption of Membranes of Extracellular Vesicles Is Necessary for ELISA Determination of Urine AQP2: Proof of Disruption and Epitopes of AQP2 Antibodies., PMID:27681727
β3 adrenergic receptor in the kidney may be a new player in sympathetic regulation of renal function., PMID:27206969
An immunoassay for urinary extracellular vesicles., PMID:26823283
Dot1l deficiency leads to increased intercalated cells and upregulation of V-ATPase B1 in mice., PMID:26404731
Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture., PMID:26147297
Role of angiotensin II and oxidative stress on renal aquaporins expression in hypernatremic rats., PMID:24590923
Elevated cAMP increases aquaporin-3 plasma membrane diffusion., PMID:24452376
Vasopressin induces apical expression of caveolin in rat kidney collecting duct principal cells., PMID:24133120
Quantification of human urinary exosomes by nanoparticle tracking analysis., PMID:24060994
Immunolocalization of aquaporin water channels in the domestic cat male genital tract., PMID:23826797
Application of phage display for ligand peptidomics to identify peptide ligands binding to AQP2-expressing membrane fractions., PMID:23765627
Long-term vasopressin-V2-receptor stimulation induces regulation of aquaporin 4 protein in renal inner medulla and cortex of Brattleboro rats., PMID:23615760
Proposed mechanism in the change of cellular composition in the outer medullary collecting duct during potassium homeostasis., PMID:23059887
Decreased expression of aquaporin 2 in the collecting duct of mice lacking the vasopressin V1a receptor., PMID:22968856
Vasopressin increases S261 phosphorylation in AQP2-P262L, a mutant in recessive nephrogenic diabetes insipidus., PMID:22778181
Immunofluorescent localization of the Rab-GAP protein TBC1D4 (AS160) in mouse kidney., PMID:22466139
Effect of Poria cocos on hypertonic stress-induced water channel expression and apoptosis in renal collecting duct cells., PMID:22414475
Characterization of Aquaporin 4 Protein Expression and Localization in Tissues of the Dogfish (Squalus acanthias)., PMID:22363294
Architecture of kangaroo rat inner medulla: segmentation of descending thin limb of Henle's loop., PMID:22237592
Daily variance of urinary excretion of AQP2 determined by sandwich ELISA method., PMID:22160633
Immunohistochemical identification of kidney nephron segments in the dog, rat, mouse, and cynomolgus monkey., PMID:22006284
Identification of new signaling components in the sensory epithelium of human saccule., PMID:21886636
Slc26a11, a chloride transporter, localizes with the vacuolar H(+)-ATPase of A-intercalated cells of the kidney., PMID:21716257
Functional characterization of three aquaglyceroporins from Trypanosoma brucei in osmoregulation and glycerol transport., PMID:21471730
Molecular mechanisms of angiotensin II stimulation on aquaporin-2 expression and trafficking., PMID:21325494
Routes of epithelial water flow: aquaporins versus cotransporters., PMID:21112289
Have we finally identified an autoimmune demyelinating disease?, PMID:20437595
Quantitative analysis of aquaporin-2 phosphorylation., PMID:20089674
Amiloride blocks lithium entry through the sodium channel thereby attenuating the resultant nephrogenic diabetes insipidus., PMID:19367330
Potential role of purinergic signaling in lithium-induced nephrogenic diabetes insipidus., PMID:19244398
Identification of phosphorylation-dependent binding partners of aquaporin-2 using protein mass spectrometry., PMID:19209902
Effects of receptor-mediated endocytosis and tubular protein composition on volume retention in experimental glomerulonephritis., PMID:19193726
For research use only. Not for human or drug use.
