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Anti-GAPDH Antibody (R2Y76)

Catalog #:   RHC09001 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG1
Applications: FCM, IF, IHC, WB
Accession: P04406
Overview

Catalog No.

RHC09001

Species reactivity

Human, Mouse, Rat, Rabbit, Monkey

Host species

Mouse

Isotype

IgG1

Clonality

Monoclonal

Tested applications

FCM: 1:200-1:400, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:5000-1:20000

Target

GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P04406

Applications

FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R2Y76

Data Image
  • Flow cytometric
    Flow cytometric analysis of NIH3T3 cells using GAPDH mouse mAb (green) and negative control (red).
  • Flow cytometric
    Flow cytometric analysis of COS7 cells using GAPDH mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Confocal Immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
  • Immunofluorescence
    Immunofluorescence analysis of NIH3T3 cells using GAPDH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse colon(A) Mouse pancreas(B) using GAPDH mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat stomach using GAPDH mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit pancreas(A) Rabbit prostate(B) using GAPDH mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using GAPDH mouse mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.
References

Maternal transfer of anti-GAPDH IgG prevents neonatal infections caused by Staphylococcus aureus and group B Streptococcus., PMID:40241760

Induction of interleukin 21 receptor expression via enhanced intracellular metabolism in B cells and its relevance to the disease activity in systemic lupus erythematosus., PMID:39740932

Evaluating the role of active TGF-β1 as inflammatory biomarker in Kashmiri (North-Indian) patients with systemic sclerosis: a case-control study., PMID:39716300

A click chemistry-based biorthogonal approach for the detection and identification of protein lysine malonylation for osteoarthritis research., PMID:39713453

CKAP4 in hepatocellular carcinoma: competitive RETREG1/FAM134B binding, reticulophagy regulation, and cancer progression., PMID:39689859

Correction to "Increased expression of desmin and vimentin reduces bladder smooth muscle contractility via JNK2"., PMID:39652015

Pioneering molecular screening for cervical precursor lesions and cervical cancer in sera., PMID:39610929

The immune responses elicited by six recombinant antigens of Mycoplasma hyopneumoniae in mice., PMID:39531743

Binding of glyceraldehyde-3-phosphate dehydrogenase to G-actin promotes the transnitrosylation reaction., PMID:39481743

Distinct Non-Human Leukocyte Antigen Antibody Signatures Correlate with Endothelial Crossmatch Status in Lung and Renal Transplant Recipients., PMID:39408890

FXR, MRP-1 and SLC7A5: New Targets for the Treatment of Hepatocellular Carcinoma., PMID:39194338

Cord blood methylation at TNFRSF17 is associated with early allergic phenotypes., PMID:39085570

Resolving the current controversy of use and reuse of housekeeping proteins in ageing research: Focus on saving people's tax dollars., PMID:39067773

Regulation of AMPK and GAPDH by Transglutaminase 2 Plays a Pivotal Role in Microvascular Leakage in Diabetic Retinas., PMID:39024127

A fungal-binding agglutinin in the skin slime of Japanese flounder (Paralichthys olivaceus) is glyceraldehyde 3-phosphate dehydrogenase., PMID:38923675

The rheumatoid arthritis drug auranofin exerts potent anti-lymphoma effect by stimulating TXNRD-mediated ROS generation and inhibition of energy metabolism., PMID:38909408

AMPK drives both glycolytic and oxidative metabolism in murine and human T cells during graft-versus-host disease., PMID:38810258

Label-Free Quantitative Analysis of Pig Liver Proteome after Hepatitis E Virus Infection., PMID:38543773

Autoimmune retinopathy in a patient with smoldering multiple myeloma: a case report., PMID:38363513

A real-time PCR for quantification of parasite burden and its correlations with clinical characteristics and anti-rKRP42 IgG level in cutaneous leishmaniasis in Sri Lanka., PMID:38341021

[Corrigendum] Long non‑coding RNA UCA1 confers tamoxifen resistance in breast cancer endocrinotherapy through regulation of the EZH2/p21 axis and the PI3K/AKT signaling pathway., PMID:38214398

Network analysis of H. pylori effect on AGS human gastric adenocarcinoma cells gene expression profile., PMID:40406433

An antibody-free evaluation of an mRNA COVID-19 vaccine., PMID:38096736

Senecavirus A induces mitophagy to promote self-replication through direct interaction of 2C protein with K27-linked ubiquitinated TUFM catalyzed by RNF185., PMID:38084826

Autophagy activates EGR1 via MAPK/ERK to induce FGF2 in renal tubular cells for fibroblast activation and fibrosis during maladaptive kidney repair., PMID:37978868

Comparative analyses of functional antibody-mediated inhibition with anti-circumsporozoite monoclonal antibodies against transgenic Plasmodium berghei., PMID:37936181

Autoimmune anti-D in an RhD-positive young infant: Learning from a rare case., PMID:37930933

Fluorescence-tagged salivary small extracellular vesicles as a nanotool in early diagnosis of Parkinson's disease., PMID:37667227

Evaluation of androgen receptor markers in erectile dysfunction., PMID:37551851

AMPK Drives Both Glycolytic and Oxidative Metabolism in T Cells During Graft-versus-host Disease., PMID:37398326

S-nitrosylation and S-glutathionylation of GAPDH: Similarities, differences, and relationships., PMID:37355052

Bovine Herpesvirus-4 Based Vaccine Provides Protective Immunity against Streptococcus suis Disease in a Rabbit Model., PMID:37243109

Quantification of SARS-CoV-2 spike protein expression from mRNA vaccines using isotope dilution mass spectrometry., PMID:37202272

Hijacking of Host Plasminogen by Mesomycoplasma (Mycoplasma) hyopneumoniae via GAPDH: an Important Virulence Mechanism To Promote Adhesion and Extracellular Matrix Degradation., PMID:37199643

Performance benchmarking microplate-immunoassays for quantifying target-specific cysteine oxidation reveals their potential for understanding redox-regulation and oxidative stress., PMID:37192685

Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes., PMID:37189694

Activation of the toll-like receptor 2 signaling pathway by GAPDH from bacterial strain RD055328., PMID:37169920

VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation., PMID:37114564

Differential patterns of fish sensitization in Asian populations: Implication for precision diagnosis., PMID:37032258

Glyceraldehyde-3-phosphate dehydrogenase regulates activation of c-Jun N-terminal kinase under oxidative stress., PMID:36963174

The Application of Guanidinium to Improve Biomolecule Quality in Fixed, Paraffin-embedded Tissue., PMID:36869703

Molecular Characteristics of Toxicity of Acrolein Produced from Spermine., PMID:36830667

Establishment and Application of an Indirect ELISA for the Detection of Antibodies to Porcine Streptococcus suis Based on a Recombinant GMD Protein., PMID:36830506

Novel maternal autoantibodies in autism spectrum disorder: Implications for screening and diagnosis., PMID:36816132

Screening of autoantibodies as biomarkers in the serum of renal cancer patients based on human proteome microarray., PMID:36789694

Dynamic cycling with a unique Hsp90/Hsp70-dependent chaperone machinery and GAPDH is needed for heme insertion and activation of neuronal NO synthase., PMID:36596358

[Protective effect of heat shock transcription factor 1 on acute lung injury in septic rats by regulating NOD-like receptor protein 3 inflammasome activation]., PMID:36567560

Biglycan as a mediator of proinflammatory response and target for MDS and sAML therapy., PMID:36531688

Effect of FXR agonist GW4064 in the treatment of hilar cholangiocarcinoma in rats., PMID:36344586

Significance of Mannan Binding Lectin-Associated Serine Protease 2 in Urinary Extracellular Vesicles in IgA Nephropathy., PMID:36149051

Datasheet

Document Download

Anti-GAPDH Antibody (R2Y76).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-GAPDH Antibody (R2Y76) [RHC09001]
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