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Anti-DDX3X Antibody (R2T56)

Catalog #:   RHA16101 Specific References (19) DATASHEET
Host species: Mouse
Isotype: IgG2a
Applications: ELISA, FCM, IF, IHC, WB
Accession: O00571
Overview

Catalog No.

RHA16101

Species reactivity

Human, Mouse, Rat, Rabbit, Monkey

Host species

Mouse

Isotype

IgG2a

Clonality

Monoclonal

Tested applications

ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:50-1:500, IHC: 1:100-1:500, WB: 1:500-1:2000

Target

DEAD box, X isoform, CAP-Rf, DDX3, HLP2, DEAD box protein 3, X-chromosomal, DDX3X, ATP-dependent RNA helicase DDX3X, Helicase-like protein 2, DBX

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O00571

Applications

ELISA, FCM, IF, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% Sodium Azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

R2T56

Data Image
  • Flow Cytometry
    Flow cytometric analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).
  • Flow Cytometry
    Flow cytometric analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunofluorescence
    Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using DDX3X mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using DDX3X mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using DDX3X mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Mouse kidney using DDX3X mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rat kidney using DDX3X mouse mAb with DAB staining.
  • Immunohistochemical
    Immunohistochemical analysis of paraffin-embedded Rabbit kidney using DDX3X mouse mAb with DAB staining.
  • Western blot
    Western blot analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.
  • Western blot
    Western blot analysis using DDX3X mouse mAb against PC-12(1) Raw264.7(2)NIH/3T3(3)NRK(4)C6(5)F9(6)COS-7(&)CHO3D10(8) cell lysate.
References

Biological and Clinical Relevance of Genetic Alterations in Peripheral T-cell Lymphomas., PMID:40416001

A genome wide CRISPR screen reveals novel determinants of long-lived plasma cell secretory capacity., PMID:40060628

The Circadian Clock Component RORA Increases Immunosurveillance in Melanoma by Inhibiting PD-L1 Expression., PMID:38718296

The 3' terminal region of Zika virus RNA contains a conserved G-quadruplex and is unfolded by human DDX17., PMID:37774422

Proteome analysis of CD5-positive diffuse large B cell lymphoma FFPE tissue reveals downregulation of DDX3X, DNAJB1, and B cell receptor signaling pathway proteins including BTK and Immunoglobulins., PMID:37705009

Copy Number Alteration and Mutational Profile of High-Grade B-Cell Lymphoma with MYC and BCL2 and/or BCL6 Rearrangements, Diffuse Large B-Cell Lymphoma with MYC-Rearrangement, and Diffuse Large B-Cell Lymphoma with MYC-Cluster Amplification., PMID:36497332

RNA G-quadruplex structures control ribosomal protein production., PMID:34815422

Aluminum impairs cognitive function by activating DDX3X-NLRP3-mediated pyroptosis signaling pathway., PMID:34614429

The protein landscape of chronic lymphocytic leukemia., PMID:34189564

Outcomes by Clinical and Molecular Features in Children With Medulloblastoma Treated With Risk-Adapted Therapy: Results of an International Phase III Trial (SJMB03)., PMID:33405951

Combination of anti-PD-1 antibody with P-GEMOX as a potentially effective immunochemotherapy for advanced natural killer/T cell lymphoma., PMID:33376237

DDX3 Participates in Translational Control of Inflammation Induced by Infections and Injuries., PMID:30373933

Medulloblastoma, WNT-activated/SHH-activated: clinical impact of molecular analysis and histogenetic evaluation., PMID:29582169

The YAP1/SIX2 axis is required for DDX3-mediated tumor aggressiveness and cetuximab resistance in KRAS-wild-type colorectal cancer., PMID:28435452

Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells., PMID:27300509

Modulation of the type I interferon pathways by culture-adaptive hepatitis C virus core mutants., PMID:22616990

Characterization of the endothelial cell cytoskeleton following HLA class I ligation., PMID:22247778

Combined CD4 T-cell and antibody response to human minor histocompatibility antigen DBY after allogeneic stem-cell transplantation., PMID:21709606

Human DDX3 functions in translation and interacts with the translation initiation factor eIF3., PMID:18628297

Datasheet

Document Download

Anti-DDX3X Antibody (R2T56).pdf

 

$ 220
Product specifications
50 μg 220 100 μg 350

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Anti-DDX3X Antibody (R2T56) [RHA16101]
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