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Recombinant Human Perforin/PRF1 Protein, N-His

Catalog #:   YHD03801 Specific References (50) DATASHEET
Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Expression system: E. coli
Accession: P14222
Protein length: Gln411-Gly531
Overview

Catalog No.

YHD03801

Expression system

E. coli

Species

Homo sapiens (Human)

Protein length

Gln411-Gly531

Predicted molecular weight

15.96 kDa

Nature

Recombinant

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

P14222

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

Cytolysin, Perforin-1, Lymphocyte pore-forming protein, PFP, PRF1, P1

Data Image
  • SDS-PAGE
    SDS PAGE for recombinant Human PRF1 protein
References

Role of vitamin D supplementation and vitamin D receptor in drug-resistant epilepsy: A double-blind placebo-controlled trial conducted in India., PMID:40516032

Impact of non-digestible carbohydrates and prebiotics on immunity, infections, inflammation and vaccine responses: a systematic review of evidence in healthy humans and a discussion of mechanistic proposals., PMID:40516031

m6A-REFII: an antibody-independent tool for in situ visualizing cellular specific RNA m6A compatible with protein immunofluorescence staining., PMID:40516020

Single cell analysis and bioinformatics reveal pyroptosis mechanisms in hepatocellular carcinoma., PMID:40516009

Correction: From Crude Extracts to Purity: A Comparative Study of Butyrylcholinesterase Purification., PMID:40516008

From neurotoxicity to neuroprotection: Rethinking GABAAR-targeting anesthetics., PMID:40516005

Recent Overview of Protein Palmitoylation and Profiling Methodologies., PMID:40516001

System-Wide Profiling of Ubiquitin E3 Ligase Activities with Quantitative Proteomics and Bioinformatics Analysis., PMID:40516000

Activity-Based Protein Profiling for Functional Cysteines and Protein Target Identification., PMID:40515999

Activity-Based Profiling of Reactive Nucleophilic Cysteine Sites from Tissue Proteomes for Drug Discovery Applications., PMID:40515998

Activity-Based Protein Profiling of Bacterial Monooxygenases., PMID:40515997

A Comprehensive Data Processing Pipeline for Phosphoproteomics in Viral Infection Studies., PMID:40515996

Identification of Anticancer ROS Targets by Cysteine Reactivity Protein Profiling., PMID:40515995

Isolation of Exosome from Senescent Preadipocytes for Mass Spectrometric Analysis., PMID:40515994

CellEKT: A Chemical Proteomics Workflow to Profile Cellular Target Engagement of Kinase Inhibitors - A Step-by-Step Guide., PMID:40515993

Activity-Based Protein Profiling of Active Host Cell Serine Hydrolases for Bioprocess Development of Therapeutic Proteins and Vaccines., PMID:40515991

Activity-Based Protein Profiling to Study Virus-Host and Viral-Induced MicroRNA-Host Interactions., PMID:40515990

Application of Activity-Based Protein Profiling (ABPP) Strategy in the Identification of New Targets for Vascular Normalization and Binding Site Resolution., PMID:40515989

Identifying Inhibitor Targets in Mycobacteria by Activity-Based Probe Profiling., PMID:40515988

Microplate-Based Enzymatic Activity Assay Protocol Powered by Activity-Based Probes., PMID:40515987

An Overview of Analytical Approaches to Cancer Proteogenomics., PMID:40515986

Profiling Ligand-Induced Changes in Nuclear Localization Using Proximity Labeling-Coupled Chemoproteomics., PMID:40515985

Activity-Based Protein Profiling of Serine Hydrolases in Bacteria: Methods and Protocols., PMID:40515984

Activity-Based Protein Profiling (ABPP) of Cellular DeISGylating Enzymes and Inhibitor Screening., PMID:40515982

SAM486A-induced inhibition of AMD1: metabolic and epigenetic implications in non-small cell lung cancer cells., PMID:40515967

Prevalence and clinical significance of autoantibodies to sulphite oxidase and glycogen phosphorylase in Chinese primary biliary cholangitis patients., PMID:40515960

ZNF432 suppresses endometrial cancer progression by promoting UPF1 ubiquitination and inducing apoptosis., PMID:40515959

Updated insights on ASK1 signaling: mechanisms, regulation, and therapeutic potential in diseases., PMID:40515958

Discovery of novel compound IGYZT01060 for inhibition of IRAK-4 enzyme for the treatment of sepsis., PMID:40515955

The potential role of PAX5 and PAX8 in regulating telomerase activity: a narrative mini-review., PMID:40515947

An overview on in-vivo generation of CAR-T cells using CRISPR-loaded functionalized nanocarriers for treating B-cell lineage acute lymphoblastic leukemia., PMID:40515942

Amplified TPM1 suppresses non-small cell lung cancer cells proliferation and metastasis., PMID:40515937

Resonance assignment of the intrinsically disordered actin-binding region of Drebrin., PMID:40515930

Detection of Protein Interactions and Dynamics During Viral Infection via Fluorescence Resonance Energy Transfer., PMID:40515929

SELEX (Systematic Evolution of Ligands by Exponential Enrichment) to Identify DNA Motifs Mirroring Chromosome Targets of the Hypothetical Transcription Factor NLRP3., PMID:40515928

Applying Phage Display Technology to Obtain Specific Peptides Blocking Host-Virus Interactions., PMID:40515926

Electrophoretic Mobility Shift Assay for Identification of the Interaction Between a Viral Protein and DNA., PMID:40515925

Dual-Luciferase Reporter Assay to Investigate Virus-Host Interactions., PMID:40515924

RNA-Binding Protein Immunoprecipitation 1 (RIP) Assay to Investigate the Interactions Between Viral RNA and Host Proteins., PMID:40515923

RBD Amplicon Sequencing and Evolutionary Analysis of SARS-CoV-2 Variants from Wastewater Samples., PMID:40515922

Screening for Host Proteins with Pro- and Antiviral Activity via High-Throughput CRISPR., PMID:40515919

Zebrafish as a Model for Studying Virus-Host Interactions., PMID:40515918

Chromatin Immunoprecipitation (ChIP)-Quantitative PCR (qPCR) Analysis of Viral Genome-Host Protein Interactions., PMID:40515916

Engineering of the WSN Strain for Investigating Antiviral and Antitumor Immunity., PMID:40515913

Detection of Protein Interactions During Viral Infection by Bimolecular Fluorescence Complementation., PMID:40515912

Identification of Virus-Host Protein Interactions Via Proteomic Techniques., PMID:40515909

Pull-Down Techniques for Determining Virus-Host Protein Interactions., PMID:40515907

High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology., PMID:40515906

CRISPR-Mediated Viral Gene Knock-In for Studying Viral-Host Interactions., PMID:40515905

Computational Analysis of Virus-Host Interactomes., PMID:40515903

Datasheet
$ 313
Product specifications
100 μg 313 1 mg 1629

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For research use only. Not for human or drug use.

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Recombinant Human Perforin/PRF1 Protein, N-His [YHD03801]
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