Catalog No.
RHN67102
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
MEM3, VPS35, Maternal-embryonic 3, Vesicle protein sorting 35, Vacuolar protein sorting-associated protein 35, hVPS35
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q96QK1
Applications
FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2Q12
An Inducible Luminescent System to Explore Parkinson's Disease-Associated Genes., PMID:39273438
Probe-dependent Proximity Profiling (ProPPr) Uncovers Similarities and Differences in Phospho-Tau-Associated Proteomes Between Tauopathies., PMID:38585836
Identification of high-performing antibodies for Vacuolar protein sorting-associated protein 35 (hVPS35) for use in Western Blot, immunoprecipitation and immunofluorescence., PMID:38434631
Deciphering the LRRK code: LRRK1 and LRRK2 phosphorylate distinct Rab proteins and are regulated by diverse mechanisms., PMID:33459343
Vps35 Deficiency Impairs Cdk5/p35 Degradation and Promotes the Hyperphosphorylation of Tau Protein in Retinal Ganglion Cells., PMID:31995153
Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking., PMID:30640902
The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human., PMID:29743203
Vps35-deficiency impairs SLC4A11 trafficking and promotes corneal dystrophy., PMID:28934248
Modifiers of prion protein biogenesis and recycling identified by a highly parallel endocytosis kinetics assay., PMID:28341739
Parkinson's disease-associated mutant VPS35 causes mitochondrial dysfunction by recycling DLP1 complexes., PMID:26618722
Mining the pre-diagnostic antibody repertoire of TgMMTV-neu mice to identify autoantibodies useful for the early detection of human breast cancer., PMID:24886063
Snx3 regulates recycling of the transferrin receptor and iron assimilation., PMID:23416069
Pathogenic mutation in VPS35 impairs its protection against MPP(+) cytotoxicity., PMID:23411763
Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid β (Aβ) production in the trans-Golgi network., PMID:22711829
Protein kinase C and rho activated coiled coil protein kinase 2 (ROCK2) modulate Alzheimer's APP metabolism and phosphorylation of the Vps10-domain protein, SorL1., PMID:21192821
The epidermal growth factor receptor antibody cetuximab induces autophagy in cancer cells by downregulating HIF-1alpha and Bcl-2 and activating the beclin 1/hVps34 complex., PMID:20634405
Analysis of articulation between clathrin and retromer in retrograde sorting on early endosomes., PMID:19874558
Identification of novel retromer complexes in the mouse testis., PMID:18656452
Phosphoinositide 3-kinase regulates the role of retromer in transcytosis of the polymeric immunoglobulin receptor., PMID:17184770
Plant retromer, localized to the prevacuolar compartment and microvesicles in Arabidopsis, may interact with vacuolar sorting receptors., PMID:16582012
The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor., PMID:15247922
For research use only. Not for human or drug use.
