Catalog No.
RHK44103
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
HsCdc18, CDC18L, CDC6, HsCDC6, CDC6-related protein, Cell division control protein 6 homolog, p62(cdc6), Cdc18-related protein
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q99741
Applications
IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2M10
Significance of DNA Replication Licensing Proteins (MCM2, MCM5 and CDC6), p16 and p63 as Markers of Premalignant Lesions of the Uterine Cervix: Its Usefulness to Predict Malignant Potential., PMID:29373905
Cell Division Cycle 6 Promotes Mitotic Slippage and Contributes to Drug Resistance in Paclitaxel-Treated Cancer Cells., PMID:27611665
A Role of hIPI3 in DNA Replication Licensing in Human Cells., PMID:27057756
Targeting of cytosolic phospholipase A2α impedes cell cycle re-entry of quiescent prostate cancer cells., PMID:26416244
Cyclin-A-CDK2-mediated phosphorylation of CIZ1 blocks replisome formation and initiation of mammalian DNA replication., PMID:25736292
Phosphorylation of Cdc6 at serine 74, but not at serine 106, drives translocation of Cdc6 to the cytoplasm., PMID:23129444
Cdc45 limits replicon usage from a low density of preRCs in mammalian cells., PMID:21390258
ARRB1-mediated regulation of E2F target genes in nicotine-induced growth of lung tumors., PMID:21212384
Replication origins are already licensed in G1 arrested unfertilized sea urchin eggs., PMID:20153311
Increased origin activity in transformed versus normal cells: identification of novel protein players involved in DNA replication and cellular transformation., PMID:20064876
Low expression of a few genes indicates good prognosis in estrogen receptor positive breast cancer., PMID:19619298
The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells., PMID:18320024
Genome-wide analysis of mouse myeloma cell lines expressing therapeutic antibodies., PMID:17592857
Identification and functional analysis of a human homologue of the monkey replication origin ors8., PMID:16823771
p16INK4A, CDC6, and MCM5: predictive biomarkers in cervical preinvasive neoplasia and cervical cancer., PMID:15858126
Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts., PMID:15668171
Overexpression of the replication licensing regulators hCdt1 and hCdc6 characterizes a subset of non-small-cell lung carcinomas: synergistic effect with mutant p53 on tumor growth and chromosomal instability--evidence of E2F-1 transcriptional control over hCdt1., PMID:15466399
Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A., PMID:14749377
Immunohistochemical localization of cdc6 in squamous and glandular neoplasia of the uterine cervix., PMID:12296751
A key role of the hSNF5/INI1 tumour suppressor in the control of the G1-S transition of the cell cycle., PMID:12226744
Essential role of human CDT1 in DNA replication and chromatin licensing., PMID:11896191
Analysis of Cdc6 function in the assembly of mammalian prereplication complexes., PMID:11805305
Cdc6 expression as a marker of proliferative activity in brain tumors., PMID:11496317
DNA replication licensing and human cell proliferation., PMID:11493639
Assembly of the human origin recognition complex., PMID:11323433
Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis., PMID:11046155
Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1., PMID:10995389
CDC6 interacts with c-Myc to inhibit E-box-dependent transcription by abrogating c-Myc/Max complex., PMID:10899308
Identification and characterization of Saccharomyces cerevisiae Cdc6 DNA-binding properties., PMID:10793143
Interaction of Xenopus Cdc2 x cyclin A1 with the origin recognition complex., PMID:10660590
DNA replication in quiescent cell nuclei: regulation by the nuclear envelope and chromatin structure., PMID:10588645
Cyclin A-dependent kinase activity affects chromatin binding of ORC, Cdc6, and MCM in egg extracts of Xenopus laevis., PMID:10491086
Minichromosome maintenance proteins as biological markers of dysplasia and malignancy., PMID:10473096
Multistep regulation of DNA replication by Cdk phosphorylation of HsCdc6., PMID:10339564
Molecular changes herald a new era for cervical cancer screening., PMID:10322308
Improved cervical smear assessment using antibodies against proteins that regulate DNA replication., PMID:9843993
The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p., PMID:9829972
Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F., PMID:9774682
Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells., PMID:9520412
Is the yeast anaphase promoting complex needed to prevent re-replication during G2 and M phases?, PMID:9312056
Negative regulation of G1 and G2 by S-phase cyclins of Saccharomyces cerevisiae., PMID:7651421
For research use only. Not for human or drug use.
