Catalog No.
RHK42802
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
FCM: 1:20-1:100, IF: 1:50-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000
Target
Antigen NY-CO-7, CHIP, CLL-associated antigen KW-8, STUB1, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1, E3 ubiquitin-protein ligase CHIP, RING-type E3 ubiquitin transferase CHIP
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q9UNE7
Applications
FCM, IF, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2M02
Structures of Fab-stabilized CHIP reveal a conformational switch important in E3 ligase and chaperone functions., PMID:40463190
Recombinant antibodies inhibit enzymatic activity of the E3 ubiquitin ligase CHIP via multiple mechanisms., PMID:39863102
O-GlcNAcylation of enolase 1 serves as a dual regulator of aerobic glycolysis and immune evasion in colorectal cancer., PMID:39446384
Phosphorylation of tau at a single residue inhibits binding to the E3 ubiquitin ligase, CHIP., PMID:39266525
Peptide Degrader-Based Targeting of METTL3/14 Improves Immunotherapy Response in Cutaneous Melanoma., PMID:39136347
GABA/baclofen stabilizes PD-L1 and enhances immunotherapy of breast cancer., PMID:38601585
CHIP ameliorates cerebral ischemia-reperfusion injury by attenuating necroptosis and inflammation., PMID:34905731
GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma., PMID:34489334
CHIP/STUB1 Ubiquitin Ligase Functions as a Negative Regulator of ErbB2 by Promoting Its Early Post-Biosynthesis Degradation., PMID:34439093
Chaperone-assisted selective autophagy in healthy and papillomavirus-associated neoplastic urothelium of cattle., PMID:29981700
Identifying the substrate proteins of U-box E3s E4B and CHIP by orthogonal ubiquitin transfer., PMID:29326975
CHIP mediates down-regulation of nucleobindin-1 in preosteoblast cell line models., PMID:27178152
An Integrative Proteomic Approach Identifies Novel Cellular SMYD2 Substrates., PMID:27163177
Cytoplasmic STAT4 Promotes Antiviral Type I IFN Production by Blocking CHIP-Mediated Degradation of RIG-I., PMID:26695369
Autoantibody Profiling of Glioma Serum Samples to Identify Biomarkers Using Human Proteome Arrays., PMID:26370624
A molecular switch in the scaffold NHERF1 enables misfolded CFTR to evade the peripheral quality control checkpoint., PMID:25990958
CHIP functions as a novel suppressor of tumour angiogenesis with prognostic significance in human gastric cancer., PMID:22535373
miR-764-5p promotes osteoblast differentiation through inhibition of CHIP/STUB1 expression., PMID:22407479
Carboxyl terminus of Hsp70-interacting protein (CHIP) contributes to human glioma oncogenesis., PMID:21272160
C-terminus of heat shock cognate 70 interacting protein increases following stroke and impairs survival against acute oxidative stress., PMID:20677910
CHIP suppresses polyglutamine aggregation and toxicity in vitro and in vivo., PMID:16207874
Ubiquitylation of neuronal nitric-oxide synthase by CHIP, a chaperone-dependent E3 ligase., PMID:15466472
ErbB2 degradation mediated by the co-chaperone protein CHIP., PMID:12574167
For research use only. Not for human or drug use.
