Catalog No.
RHG93801
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
SP-H antigen, NMP-22, Nuclear matrix protein-22, NMP22, NuMA protein, Nuclear mitotic apparatus protein, NUMA, Nuclear mitotic apparatus protein 1, NUMA1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q14980
Applications
IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2F83
Golimumab-Induced Anti-NuMA-1 (Nuclear Mitotic Apparatus Protein 1) Antibodies in a Rheumatoid Arthritis Patient: A Case Report., PMID:39628740
Anti-NuMA antibodies: clinical associations and significance in patients with primary Sjögren's syndrome or systemic lupus erythematosus., PMID:33404653
Establishment of international autoantibody reference standards for the detection of autoantibodies directed against PML bodies, GW bodies, and NuMA protein., PMID:32776893
Global Interactomics Connect Nuclear Mitotic Apparatus Protein NUMA1 to Influenza Virus Maturation., PMID:30572664
Electrochemical ELISA-based platform for bladder cancer protein biomarker detection in urine., PMID:30005382
NuMA phosphorylation by CDK1 couples mitotic progression with cortical dynein function., PMID:23921553
Rad53 homologue forkhead-associated kinase A (FhkA) and Ca2+-binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium., PMID:23587254
Autoantibodies to 60kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern., PMID:23357050
Assessment of autoantibodies to meningioma in a population-based study., PMID:23221727
Influencing factors on the NMP-22 urine assay: an experimental model., PMID:22928931
Survival and differentiation of human embryonic stem cell-derived neural precursors grafted spinally in spinal ischemia-injured rats or in naive immunosuppressed minipigs: a qualitative and quantitative study., PMID:22889456
GAS41 amplification results in overexpression of a new spindle pole protein., PMID:22619067
Poly(ADP-ribose) polymerase 3 (PARP3), a newcomer in cellular response to DNA damage and mitotic progression., PMID:21270334
Robust control of mitotic spindle orientation in the developing epidermis., PMID:21098114
Anti-NuMA1 and anti-NuMA2 (anti-HsEg5) antibodies: Clinical and immunological features: A propos of 40 new cases and review of the literature., PMID:20457279
Defining the molecular target of an antibody derived from nuclear extract of Jurkat cells using protein arrays., PMID:19733143
Redefining the Scl-70 indirect immunofluorescence pattern: autoantibodies to DNA topoisomerase I yield a specific compound immunofluorescence pattern., PMID:19395540
Identification and characterization of INMAP, a novel interphase nucleus and mitotic apparatus protein that is involved in spindle formation and cell cycle progression., PMID:19331820
Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole., PMID:18832153
The spindle function of CDCA4., PMID:18498124
Antibodies to mitotic spindle apparatus: clinical significance of NuMA and HsEg5 autoantibodies., PMID:18240009
The control of tissue architecture over nuclear organization is crucial for epithelial cell fate., PMID:17405811
Inactivation of MAPK affects centrosome assembly, but not actin filament assembly, in mouse oocytes maturing in vitro., PMID:17219430
NuMA influences higher order chromatin organization in human mammary epithelium., PMID:17108325
Autoantibodies to mitotic apparatus: association with other autoantibodies and their clinical significance., PMID:16941236
The Nuclear Mitotic Apparatus (NuMA) protein is contributed by the donor cell nucleus in cloned porcine embryos., PMID:16368570
Dynamic changes in NuMA and microtubules in monkey-rabbit nuclear transfer embryos., PMID:16368565
Caspase-3 is required in the apoptotic disintegration of the nuclear matrix., PMID:16199031
Function of donor cell centrosome in intraspecies and interspecies nuclear transfer embryos., PMID:15878330
Multiple mechanisms regulate NuMA dynamics at spindle poles., PMID:15561764
XRHAMM functions in ran-dependent microtubule nucleation and pole formation during anastral spindle assembly., PMID:15498487
NuMA expression and function in mouse oocytes and early embryos., PMID:15067221
XMAP215, XKCM1, NuMA, and cytoplasmic dynein are required for the assembly and organization of the transient microtubule array during the maturation of Xenopus oocytes., PMID:14499655
[Clinical significance of fluoroscopic patterns specific for the mitotic spindle in patients with rheumatic diseases]., PMID:12404031
Unraveling the organization of the internal nuclear matrix: RNA-dependent anchoring of NuMA to a lamin scaffold., PMID:12243746
Pim-1 associates with protein complexes necessary for mitosis., PMID:12111331
A mammalian Partner of inscuteable binds NuMA and regulates mitotic spindle organization., PMID:11781568
Further considerations on the intranuclear distribution of HMGI/Y proteins., PMID:11767201
In vitro induction and in vivo expression of bcl-2 in the hNT neurons., PMID:11704352
A potential role for human cohesin in mitotic spindle aster assembly., PMID:11590136
Immunolocalization of NuMA and phosphorylated proteins during the cell cycle in human breast and prostate cancer cells as analyzed by immunofluorescence and postembedding immunoelectron microscopy., PMID:11449886
Gender-dependent specific immune response during chronic human Schistosomiasis haematobia., PMID:11359443
The effects of taurine on hNT neurons transplanted in adult rat striatum., PMID:11202562
Chromosome movement in mitosis requires microtubule anchorage at spindle poles., PMID:11157972
GAS41, a highly conserved protein in eukaryotic nuclei, binds to NuMA., PMID:10913114
Spindle formation and dynamics of gamma-tubulin and nuclear mitotic apparatus protein distribution during meiosis in pig and mouse oocytes., PMID:10775165
Chromosomal instability and cytoskeletal defects in oral cancer cells., PMID:10618413
Ran-GTP stabilises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts., PMID:10381400
Influence of different metal ions on the ultrastructure, biochemical properties, and protein localization of the K562 cell nuclear matrix., PMID:10321834
The plant nucleoskeleton: ultrastructural organization and identification of NuMA homologues in the nuclear matrix and mitotic spindle of plant cells., PMID:9925768
For research use only. Not for human or drug use.
