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Anti-PUM1 Antibody (R2F65)

Catalog #:   RHG86201 Specific References (6) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, IHC, IP, WB
Accession: Q14671
Overview

Catalog No.

RHG86201

Species reactivity

Human, Mouse

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000

Target

Pumilio-1, HsPUM, KIAA0099, Pumilio homolog 1, PUM1, PUMH1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q14671

Applications

IF, IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R2F65

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of PUM1 (green) in HCT116 using PUM1 antibody,and DAPI(blue).
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human tonsil using Pumilio 1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot
    Western blot analysis of Pumilio 1 in 3T3 lysates using Pumilio 1 antibody.
References

PUM1 Is Overexpressed in Colon Cancer Cells With Acquired Resistance to Cetuximab., PMID:34447749

Evidence for a centrosome-attracting body like structure in germ-soma segregation during early development, in the urochordate Oikopleura dioica., PMID:29486709

RNA-binding protein immunopurification-microarray (RIP-Chip) analysis to profile localized RNAs., PMID:21431753

Biochemical characterization of Pumilio1 and Pumilio2 in Xenopus oocytes., PMID:21098481

Involvement of Xenopus Pumilio in the translational regulation that is specific to cyclin B1 mRNA during oocyte maturation., PMID:12963108

Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein., PMID:11283000

Datasheet

Document Download

Anti-PUM1 Antibody (R2F65).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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For research use only. Not for human or drug use.

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Anti-PUM1 Antibody (R2F65) [RHG86201]
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