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Anti-TRIM25 Antibody (R2F33)

Catalog #:   RHG77201 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, IP, WB
Accession: Q14258
Overview

Catalog No.

RHG77201

Species reactivity

Human, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IP: 1:20, WB: 1:500-1:1000

Target

E3 ubiquitin/ISG15 ligase TRIM25, RING-type E3 ubiquitin transferase TRIM25, Tripartite motif-containing protein 25, EFP, Ubiquitin/ISG15-conjugating enzyme TRIM25, TRIM25, Zinc finger protein 147, RING finger protein 147, RNF147, ZNF147, RING-type E3 ubiquitin transferase, Estrogen-responsive finger protein

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q14258

Applications

IF, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R2F33

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of TRIM25/EFP (green) in MCF-7 using TRIM25/EFP antibody,and DAPI(blue)
  • Western blot
    Western blot analysis of TRIM25/EFP in K562, rat Brain, C6, Hela lysates using TRIM25 antibody.
References

Combined use of immunoreactivities of Efp and ZCCHC3 for predicting prognosis of patients with triple-negative breast cancer., PMID:39936817

Interleukin-22 facilitates the interferon-λ-mediated production of tripartite motif protein 25 to inhibit replication of duck viral hepatitis A virus type 1., PMID:37391858

IFN-I inducible miR-3614-5p targets ADAR1 isoforms and fine tunes innate immune activation., PMID:35935998

TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation., PMID:35266845

Combined Use of Immunoreactivities of RIG-I with Efp/TRIM25 for Predicting Prognosis of Patients With Estrogen Receptor-positive Breast Cancer., PMID:33386231

TLR3 Activation of Hepatic Stellate Cell Line Suppresses HBV Replication in HepG2 Cells., PMID:30619284

Dynamic Modulation of Expression of Lentiviral Restriction Factors in Primary CD4+ T Cells following Simian Immunodeficiency Virus Infection., PMID:28100613

Associations between polymorphisms in the antiviral TRIM genes and measles vaccine immunity., PMID:23416095

Negative role of RIG-I serine 8 phosphorylation in the regulation of interferon-beta production., PMID:20406818

Phosphorylation-mediated negative regulation of RIG-I antiviral activity., PMID:20071582

Expression of estrogen-responsive finger protein (Efp) is associated with advanced disease in human epithelial ovarian cancer., PMID:16140366

Molecular cloning of rat efp: expression and regulation in primary osteoblasts., PMID:10425199

Datasheet

Document Download

Anti-TRIM25 Antibody (R2F33).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-TRIM25 Antibody (R2F33) [RHG77201]
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