Catalog No.
RHG74704
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
WB: 1:1000-1:2000
Target
RecQ protein-like 2, DNA helicase, RecQ-like type 3, RecQ3, Werner syndrome ATP-dependent helicase, RECQ3, RECQL2, Exonuclease WRN, WRN
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q14191
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2F25
Integrated liver and serum proteomics uncover sexual dimorphism and alteration of several immune response proteins in an aging Werner syndrome mouse model., PMID:38795389
Werner helicase is required for proliferation and DNA damage repair in multiple myeloma., PMID:36515823
Werner syndrome presenting as early-onset diabetes: A case report., PMID:34564935
Characterization of G-quadruplex antibody reveals differential specificity for G4 DNA forms., PMID:33084858
WRN-Mutated Colorectal Cancer Is Characterized by a Distinct Genetic Phenotype., PMID:32455893
Custom DNA Microarrays Reveal Diverse Binding Preferences of Proteins and Small Molecules to Thousands of G-Quadruplexes., PMID:32216326
Functional conservation of RecQ helicase BLM between humans and Drosophila melanogaster., PMID:31772289
Flow Cytometry as a Diagnostic Tool in Primary and Secondary Immune Deficiencies., PMID:31668272
Reprogramming of Human Peripheral Blood Mononuclear Cell (PBMC) from a patient suffering of a Werner syndrome resulting in iPSC line (REGUi003-A) maintaining a short telomere length., PMID:31404747
Patient-Derived Xenografts and Matched Cell Lines Identify Pharmacogenomic Vulnerabilities in Colorectal Cancer., PMID:31375513
Prevention of mother-to-child transmission of hepatitis B virus: a phase III, placebo-controlled, double-blind, randomized clinical trial to assess the efficacy and safety of a short course of tenofovir disoproxil fumarate in women with hepatitis B virus e-antigen., PMID:27506549
Metabolic and Phenotypic Differences between Mice Producing a Werner Syndrome Helicase Mutant Protein and Wrn Null Mice., PMID:26447695
BLM helicase facilitates telomere replication during leading strand synthesis of telomeres., PMID:26195664
WRN protein as a novel erythroblast immunohistochemical marker with applications for the diagnosis of Werner syndrome., PMID:25503078
Werner complex deficiency in cells disrupts the Nuclear Pore Complex and the distribution of lamin B1., PMID:24050918
Mechanism of DNA resection during intrachromosomal recombination and immunoglobulin class switching., PMID:23254285
Triplex DNA-binding proteins are associated with clinical outcomes revealed by proteomic measurements in patients with colorectal cancer., PMID:22682314
The RAD9-RAD1-HUS1 (9.1.1) complex interacts with WRN and is crucial to regulate its response to replication fork stalling., PMID:22002307
ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery., PMID:20802463
Fibroblasts from Werner syndrome patients: cancer cells derived by experimental introduction of oncogenes maintain malignant properties despite entering crisis., PMID:20043098
DNA-dependent protein kinase (DNA-PK)-dependent cisplatin-induced loss of nucleolar facilitator of chromatin transcription (FACT) and regulation of cisplatin sensitivity by DNA-PK and FACT., PMID:19372586
Werner syndrome helicase (WRN), nuclear DNA helicase II (NDH II) and histone gammaH2AX are localized to the centrosome., PMID:17498979
The Werner syndrome protein is required for recruitment of chromatin assembly factor 1 following DNA damage., PMID:17173071
Significant elevation of IgG anti-WRN (RecQ3 RNA/DNA helicase) antibody in systemic sclerosis., PMID:16906373
Formation of a nuclear complex containing the p53 tumor suppressor, YB-1, and the Werner syndrome gene product in cells treated with UV light., PMID:16584908
Expression of Werner and Bloom syndrome genes is differentially regulated by in vitro HIV-1 infection of peripheral blood mononuclear cells., PMID:15498034
Radiosensitivity is predicted by DNA end-binding complex density, but not by nuclear levels of band components., PMID:15450732
RECQL4, mutated in the Rothmund-Thomson and RAPADILINO syndromes, interacts with ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway., PMID:15317757
Werner protein stimulates topoisomerase I DNA relaxation activity., PMID:14612507
DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97/VCP., PMID:12937274
Characterization of the slow-growth phenotype of S. cerevisiae Whip/Mgs1 Sgs1 double deletion mutants., PMID:12509289
Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases., PMID:12181313
The processing of Holliday junctions by BLM and WRN helicases is regulated by p53., PMID:12080066
Diagnosis of Werner syndrome by immunoblot analysis., PMID:11952711
Functional interaction of p53 and BLM DNA helicase in apoptosis., PMID:11399766
Evidence for a replication function of FFA-1, the Xenopus orthologue of Werner syndrome protein., PMID:11238454
Selective cleavage of BLM, the bloom syndrome protein, during apoptotic cell death., PMID:11154689
Covalent modification of the Werner's syndrome gene product with the ubiquitin-related protein, SUMO-1., PMID:10806190
Identification of phage antibodies toward the Werner protein by selection on Western blots., PMID:10726750
Localization of the Bloom syndrome helicase to punctate nuclear structures and the nuclear matrix and regulation during the cell cycle: comparison with the Werner's syndrome helicase., PMID:10569803
Expression of the BLM gene in human haematopoietic cells., PMID:10540192
Immunological diagnosis of Werner syndrome by down-regulated and truncated gene products., PMID:10543396
The Werner syndrome protein is involved in RNA polymerase II transcription., PMID:10436020
Werner syndrome helicase contains a 5'-->3' exonuclease activity that digests DNA and RNA strands in DNA/DNA and RNA/DNA duplexes dependent on unwinding., PMID:10325426
Detection by epitope-defined monoclonal antibodies of Werner DNA helicases in the nucleoplasm and their upregulation by cell transformation and immortalization., PMID:9885239
Werner helicase is localized to transcriptionally active nucleoli of cycling cells., PMID:9683536
Nucleolar localization of the Werner syndrome protein in human cells., PMID:9618508
Accelerated aging and nucleolar fragmentation in yeast sgs1 mutants., PMID:9271578
For research use only. Not for human or drug use.
