Catalog No.
YHC88301
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Ile923-Arg1148
Predicted molecular weight
28.81 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P11388
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
DNA topoisomerase II, alpha isozyme, TOP2A, DNA topoisomerase 2-alpha, TOP2
The Putative Packaging ATPase of Acanthamoeba polyphaga Mimivirus Is an Essential Protein That Interacts With the Genome Segregation and Packaging Machinery., PMID:40512099
Protein Biomarkers Enable Sensitive and Specific Cervical Intraepithelial Neoplasia (CIN) II/III+ Detection: One Step Closer to Universal Cervical Cancer Screening., PMID:40507244
Structural insight in understanding the impact of mutation at position 88 and 94 of DNA gyrase A of Mycobacterium tuberculosis in developing resistance against delafloxacin., PMID:40495004
Unveiling the Therapeutic Potential of Targeting RRM2 in Hepatocellular Carcinoma: An Integrated In Silico and In Vitro Study., PMID:40493217
Mitotic genome folding., PMID:40492990
Topoisomerase II Inhibition in Cancer: A Focus on Metal Complexes., PMID:40491376
Exploring the anti-gastric cancer mechanisms of Diosgenin through integrated network analysis, bioinformatics, single-cell sequencing, and cell experiments., PMID:40487391
Tyrosine and Phenylalanine Activate Neuronal DNA Repair but Exhibit Opposing Effects on Global Transcription and Adult Female Mice Are Resilient to TyrRS/YARS1 Depletion., PMID:40476370
Tetrahydropyrido[4,3-d]pyrimidines as a new active scaffold for human topoisomerase II inhibitors., PMID:40467713
ICRF193 potentiates the genotoxicity of etoposide., PMID:40467706
Identification of potential biomarkers associated with immune cell infiltration patterns in Kawasaki disease via bioinformatics., PMID:40455761
YTHDF1-mediated m6A modification of TOP2A drives pulmonary hypertension via the PI3K/Akt/mTOR pathway., PMID:40451562
In-vitro and in-silico antibacterial and antibiofilm activities of an aromatic heterocyclic metabolite from a novel halo-thermophilic Streptomyces sp. strain CBN-1 against bacteria causing nosocomial infections., PMID:40448741
Starch and polyacrylic acid doped tin ferrite nanocubes for catalytic, water oxidation, and antimicrobial activities inhibiting DNA gyrase with molecular docking., PMID:40441564
Deciphering linezolid-induced hematologic toxicity: Targeting TOP2A and TOP2B via its primary metabolite PNU142586., PMID:40435237
Key Ferroptosis Genes and their Predictive and Diagnostic Value in Fanconi Anemia., PMID:40432442
Virtual Screening and Molecular Dynamics Simulation Targeting the ATP Domain of African Swine Fever Virus Type II DNA Topoisomerase., PMID:40431693
New Dimethoxyaryl-Sesquiterpene Derivatives with Cytotoxic Activity Against MCF-7 Breast Cancer Cells: From Synthesis to Topoisomerase I/II Inhibition and Cell Death Mechanism Studies., PMID:40429684
Topobexin targets the Topoisomerase II ATPase domain for beta isoform-selective inhibition and anthracycline cardioprotection., PMID:40425539
An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE., PMID:40424468
Synthesis of Novel Thiazole/Thiadiazole Conjugates of Fluoroquinolones as Potent Antibacterial and Antimycobacterial Agents., PMID:40415348
Heteroarylcyanovinyl Benzimidazoles as New Antibacterial Skeleton with Large Potential To Combat Bacterial Infections., PMID:40415272
Decoding the function of cancer-associated fibroblasts in osteosarcoma: Molecular pathways, therapeutic approaches and prognostic significance., PMID:40404112
Design and Synthesis of O-Dialkylaminoalkyl Substituted Urolithin Derivatives: DNA Topoisomerase IIα Inhibition With Promising Antiproliferative Activity., PMID:40401699
Synthesis, in vitro and in silico studies of a novel chrysin-ferrocene Schiff base with potent anticancer activity via G1 arrest, caspase-dependent apoptosis and inhibition of topoisomerase II., PMID:40396612
Exploring Thiazole-Based Heterocycles: Synthesis, Bioactivity, and Molecular Docking for Antimicrobial Applications., PMID:40395194
Recombinant Topoisomerase 2 Production Using Cultured Human Cell Lines., PMID:40372648
Kinetoplast DNA Decatenation Assay with Eukaryotic Topoisomerase II., PMID:40372647
Analyzing Topoisomerase II Cleavage Complexes Using Flow Cytometry., PMID:40372646
Inducing and Monitoring Liquid-Liquid Phase Separation by Type II Topoisomerases., PMID:40372645
Yeast Tools for Studying Type II Topoisomerases in Budding Yeast., PMID:40372643
Topoisomerase II N-terminal ATPase Clamp Stabilization., PMID:40372642
Plasmid DNA Binding Electrophoretic Mobility Shift Assay with Eukaryotic Topoisomerase II., PMID:40372641
Topoisomerase II DNA Cleavage Assay Using Fluorescently Labelled Double-Stranded Oligonucleotides., PMID:40372640
Plasmid DNA Cleavage Assay with Eukaryotic Topoisomerase II., PMID:40372639
Expression, Purification, and Measurement of DNA Supercoil Relaxation Activity by Eukaryotic Topoisomerase II., PMID:40372638
DNA Cleavage Mediated by Bacterial Type II Topoisomerases., PMID:40372637
DNA Decatenation Catalyzed by Bacterial Topoisomerase IV., PMID:40372636
DNA Supercoiling Catalyzed by Bacterial Gyrase., PMID:40372635
Clinical evaluation of personalized Helicobacter pylori treatment guided by PCR detection from fecal samples: a real-world study., PMID:40357402
A weight of evidence approach to assess the human genotoxicity risk of Chinese goldthread botanical extract and its primary constituent berberine., PMID:40354871
Coumarin hybrids: dual-target candidates for future antimicrobial and antitubercular therapies., PMID:40353302
Integrative PPI network and random forest analysis identifies KIF4A, TOP2A, and ASPM protein macromolecules as novel protein biomarkers in renal carcinoma pathogenesis., PMID:40345279
Mutagenesis of Intrinsically Disordered Domain Impacts Topoisomerase IIα Catalytic Activity., PMID:40332112
PKG-Mediated Phosphorylation of TOP2A Activates HDAC to Drive Photoreceptor Cell Death in rd1 Mouse Inherited Retinal Degeneration., PMID:40320856
Identification and validation of susceptibility modules and hub genes of adrenocortical carcinoma through WGCNA and machine learning., PMID:40317315
Vaping-induced Oxidation of CBD Causes Adduction of TOP2A and Interferes with Cellular Proliferation., PMID:40305674
Different propensities for gate opening in gyrases and topoisomerase IV., PMID:40304180
Topoisomerase IIα-mediated stemness response in reactive astrocytes to traumatic brain injury., PMID:40303339
TMC5 as a Marker of Tumor-Associated Telocytes in Hepatocellular Carcinoma., PMID:40302342
For research use only. Not for human or drug use.
