Catalog No.
RHG61701
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
WB: 1:500-1:1000
Target
Solute carrier family 12 member 1, NKCC2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, SLC12A1, Kidney-specific Na-K-Cl symporter
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q13621
Applications
WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2E86
Dysregulation of the WNK4-SPAK/OSR1 pathway has a minor effect on baseline NKCC2 phosphorylation., PMID:37881876
Tubular epithelial progenitors are excreted in urine during recovery from severe acute kidney injury and are able to expand and differentiate in vitro., PMID:36285332
Renal denervation and CD161a immune ablation prevent cholinergic hypertension and renal sodium retention., PMID:31172810
β3 adrenergic receptor in the kidney may be a new player in sympathetic regulation of renal function., PMID:27206969
Effect of vasopressin on Na(+)-K(+)-2Cl(-) cotransporter (NKCC) and the signaling mechanisms on the murine late distal colon., PMID:26656758
Regulation of the Na(+)-K(+)-2Cl(-) cotransporter by cGMP/cGMP-dependent protein kinase I after furosemide administration., PMID:26183401
Urinary exosomes in the diagnosis of Gitelman and Bartter syndromes., PMID:25422309
Identification of moesin as NKCC2-interacting protein and analysis of its functional role in the NKCC2 apical trafficking., PMID:22708623
Cellular distribution of NKCC2 in the gastric mucosa and its response to short-term osmotic shock., PMID:22388656
Group VIA phospholipase A2 is a target for vasopressin signaling in the thick ascending limb., PMID:22218592
NKCC2 is activated in Milan hypertensive rats contributing to the maintenance of salt-sensitive hypertension., PMID:21553016
Phosphorylation and transport in the Na-K-2Cl cotransporters, NKCC1 and NKCC2A, compared in HEK-293 cells., PMID:21464992
Differential expression of Na+-Cl- cotransporter and Na+-K+-Cl- cotransporter 2 in the distal nephrons of euryhaline and seawater pufferfishes., PMID:21084680
Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cells., PMID:20713729
Expression of the sodium potassium chloride cotransporter (NKCC1) and sodium chloride cotransporter (NCC) and their effects on rat lens transparency., PMID:20458365
Mechanisms for hypercalciuria in pseudohypoaldosteronism type II-causing WNK4 knock-in mice., PMID:20181799
Functional expression of the Na-K-2Cl cotransporter NKCC2 in mammalian cells fails to confirm the dominant-negative effect of the AF splice variant., PMID:19854835
Effects of dietary salt on renal Na+ transporter subcellular distribution, abundance, and phosphorylation status., PMID:18653479
Expression of aquaporin1, 3, and 4, NKCC1, and NKCC2 in the human endolymphatic sac., PMID:18606512
Human renal cells from the thick ascending limb and early distal tubule: characterization of primary isolated and cultured cells by reverse transcription polymerase chain reaction., PMID:18331438
Characterization of a long-term rat mTAL cell line., PMID:17670898
Renal localization and function of the tight junction protein, claudin-19., PMID:17389678
Regulation of the renal-specific Na+-K+-2Cl- co-transporter NKCC2 by AMP-activated protein kinase (AMPK)., PMID:17341212
Gestational length affects a change in the transepithelial voltage and the rNKCC2 expression pattern in the ascending thin limb of Henle's loop., PMID:17237717
Novel insights regarding the operational characteristics and teleological purpose of the renal Na+-K+-Cl2 cotransporter (NKCC2s) splice variants., PMID:16157691
Sodium retention in cirrhotic rats is associated with increased renal abundance of sodium transporter proteins., PMID:15673309
Reduced renal Na+-K+-Cl- co-transporter activity and inhibited NKCC2 mRNA expression by Leptospira shermani: from bed-side to bench., PMID:15388818
Resistance of mTAL Na+-dependent transporters and collecting duct aquaporins to dehydration in 7-month-old rats., PMID:12846749
Short-term stimulation of the renal Na-K-Cl cotransporter (NKCC2) by vasopressin involves phosphorylation and membrane translocation of the protein., PMID:12732642
Time course of renal Na-K-ATPase, NHE3, NKCC2, NCC, and ENaC abundance changes with dietary NaCl restriction., PMID:12217855
Renal Na-K-Cl cotransporter NKCC2 in Dahl salt-sensitive rats., PMID:11910309
Regulation of the sodium transporters NHE3, NKCC2 and NCC in the kidney., PMID:11496061
For research use only. Not for human or drug use.
