Catalog No.
RHG08501
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000
Target
11S regulator complex subunit alpha, Proteasome activator complex subunit 1, Proteasome activator 28 subunit alpha, IGUP I-5111, Interferon gamma up-regulated I-5111 protein, PA28a, Activator of multicatalytic protease subunit 1, PSME1, IFI5111, REG-alpha, PA28alpha
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q06323
Applications
IF, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2C83
Obesity enhances the response to neoadjuvant anti-PD1 therapy in oral tongue squamous cell carcinoma., PMID:38923758
iTRAQ-based proteomic analysis of differentially expressed proteins in sera of seronegative and seropositive rheumatoid arthritis patients., PMID:34812532
Downregulation of PA28α induces proteasome remodeling and results in resistance to proteasome inhibitors in multiple myeloma., PMID:33318477
Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination., PMID:28508465
Antibody-validated proteins in inflamed islets of fulminant type 1 diabetes profiled by laser-capture microdissection followed by mass spectrometry., PMID:25329145
Blood transcriptomic biomarkers in adult primary care patients with major depressive disorder undergoing cognitive behavioral therapy., PMID:25226551
Proteasome activator complex PA28 identified as an accessible target in prostate cancer by in vivo selection of human antibodies., PMID:23918357
Molecular and functional characterization of a putative PA28γ proteasome activator orthologue in Schistosoma mansoni., PMID:23611749
The C-terminal fragment of the immunoproteasome PA28S (Reg alpha) as an early diagnosis and tumor-relapse biomarker: evidence from mass spectrometry profiling., PMID:22532226
Using proteomic approach to identify tumor-associated proteins as biomarkers in human esophageal squamous cell carcinoma., PMID:21517111
The immunoproteasome in steatohepatitis: its role in Mallory-Denk body formation., PMID:21256843
Enhancement of proteasome function by PA28α overexpression protects against oxidative stress., PMID:21098724
Anti-proteasome activator 28alpha is a novel anti-cytoplasmic antibody in patients with systemic lupus erythematosus and Sjögren's syndrome., PMID:19688289
Specific MALDI imaging and profiling for biomarker hunting and validation: fragment of the 11S proteasome activator complex, Reg alpha fragment, is a new potential ovary cancer biomarker., PMID:17939699
Comparative proteomics analysis to annexin B1 DNA and protein vaccination in mice., PMID:17027125
Anti-20S proteasome autoantibodies inhibit proteasome stimulation by proteasome activator PA28., PMID:16802355
The ubiquitin-proteasome system plays essential roles in presenting an 8-mer CTL epitope expressed in APC to corresponding CD8+ T cells., PMID:16569681
Proteasomes and antigen presentation: evidence that a KEKE motif does not promote presentation of the class I epitope SIINFEKL., PMID:16423396
Antigen presentation and immune regulatory capacity of immature and mature-enriched antigen presenting (dendritic) cells derived from human bone marrow., PMID:14969764
Autoimmune response to proteasome activator 28alpha in patients with connective tissue diseases., PMID:14760793
Differential expression of tapasin and immunoproteasome subunits in adenovirus type 5- versus type 12-transformed cells., PMID:12407112
Bipartite regulation of different components of the MHC class I antigen-processing machinery during dendritic cell maturation., PMID:11717192
Interferon gamma regulates accumulation of the proteasome activator PA28 and immunoproteasomes at nuclear PML bodies., PMID:11112687
Tissue distribution of constitutive proteasomes, immunoproteasomes, and PA28 in rats., PMID:11032729
The proteasome activator 11 S REG (PA28) and class I antigen presentation., PMID:10600633
Proteasome activator subunit PA28 alpha and related Ki antigen (PA28 gamma) are absent from the nuclear fraction purified by sucrose gradient centrifugation., PMID:10216960
Conformational changes in the 20S proteasome upon macromolecular ligand binding analyzed with monoclonal antibodies., PMID:9989942
Invasion by Salmonella typhimurium induces increased expression of the LMP, MECL, and PA28 proteasome genes and changes in the peptide repertoire of HLA-B27., PMID:9746558
Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes., PMID:9620878
Molecular properties of the proteasome activator PA28 family proteins and gamma-interferon regulation., PMID:9189757
Phosphorylation of the proteasome activator PA28 is required for proteasome activation., PMID:8780702