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Anti-UBE2I/UBC9 Antibody (R2A73)

Catalog #:   RHF60801 Specific References (5) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, IHC, IP, WB
Accession: P63279
Overview

Catalog No.

RHF60801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000

Target

UBE2I, UBC9, p18, UBCE9, Ubiquitin carrier protein 9, RING-type E3 SUMO transferase UBC9, SUMO-conjugating enzyme UBC9, Ubiquitin carrier protein I, Ubiquitin-protein ligase I, Ubiquitin-conjugating enzyme E2 I, SUMO-protein ligase

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P63279

Applications

IF, IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R2A73

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 (green) in Hela using SUMO Conjugating Enzyme UBC9 antibody,and DAPI(blue).
  • Immunohistochemical
    Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2I/UBC9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
  • Western blot
    Western blot analysis of UBE2I/UBC9 in 3T3, PC-12, MEF lysates using UBE2I/UBC9 antibody.
  • Western blot
    Western blot analysis of UBE2I/UBC9 in Hela, A549, HL-60 lysates using UBE2I/UBC9 antibody
References

Dysregulated gene expression of SUMO machinery components induces the resistance to anti-PD-1 immunotherapy in lung cancer by upregulating the death of peripheral blood lymphocytes., PMID:39211047

UBE2I regulates the nuclear translocation of hnRNPA2B1 by contributing to SUMO modification in osteoarthritis., PMID:38955308

Proteomic analysis of host cellular proteins co-immunoprecipitated with duck enteritis virus gC., PMID:34091090

PDPK1 regulates autophagosome biogenesis by binding to PIK3C3., PMID:32876514

PCGF2 negatively regulates arsenic trioxide-induced PML-RARA protein degradation via UBE2I inhibition in NB4 cells., PMID:27030546

Datasheet
$ 290
Product specifications
50 μg 290 100 μg 484

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-UBE2I/UBC9 Antibody (R2A73) [RHF60801]
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