Catalog No.
RHF54702
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000
Target
RING finger protein 75, RNF75, E3 ubiquitin-protein transferase RBX1, N-terminally processed, RBX1, E3 ubiquitin-protein ligase RBX1, Rbx1, ROC1, E3 ubiquitin-protein transferase RBX1, Protein ZYP, RING-box protein 1, Regulator of cullins 1
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P62877
Applications
FCM, IF, IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2A48
Gene dosage changes in KCTD13 result in penile and testicular anomalies via diminished androgen receptor function., PMID:36196997
A20 and RBX1 Regulate Brentuximab Vedotin Sensitivity in Hodgkin Lymphoma Models., PMID:32299816
Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II., PMID:30349055
Comparative Proteomics Reveals Strain-Specific β-TrCP Degradation via Rotavirus NSP1 Hijacking a Host Cullin-3-Rbx1 Complex., PMID:27706223
Regulator of cullins-1 expression knockdown suppresses the malignant progression of muscle-invasive transitional cell carcinoma by regulating mTOR/DEPTOR pathway., PMID:26742010
CENP-A K124 Ubiquitylation Is Required for CENP-A Deposition at the Centromere., PMID:25727006
Knockdown of regulator of cullins-1 (ROC1) expression induces bladder cancer cell cycle arrest at the G2 phase and senescence., PMID:23667514
Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells., PMID:22822056
Role of ROC1 protein in the control of cyclin D1 protein expression in skin melanomas., PMID:21300445
Fbxw7β resides in the endoplasmic reticulum membrane and protects cells from oxidative stress., PMID:21205095
Hsp90 interaction with INrf2(Keap1) mediates stress-induced Nrf2 activation., PMID:20864537
Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation., PMID:20566845
ROC1/RBX1 E3 ubiquitin ligase silencing suppresses tumor cell growth via sequential induction of G2-M arrest, apoptosis, and senescence., PMID:19509229
FBXO25-associated nuclear domains: a novel subnuclear structure., PMID:18287534
Diversity in tissue expression, substrate binding, and SCF complex formation for a lectin family of ubiquitin ligases., PMID:18203720
Ubiquitination-mediated regulation of biosynthesis of the adhesion receptor SHPS-1 in response to endoplasmic reticulum stress., PMID:14701835
For research use only. Not for human or drug use.
