Catalog No.
RHF42001
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IP: 1:20-1:50, WB: 1:1000-1:2000
Target
Syntaxin-1B1, Syntaxin-1B2, STX1B1, STX1B, Syntaxin-1B, STX1B2
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P61266
Applications
IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R2A05
Cryo-EM structures of engineered Shiga toxin-based immunogens capable of eliciting neutralizing antibodies with therapeutic potential against hemolytic uremic syndrome., PMID:40411437
Protective immunization against Enterohemorrhagic Escherichia coli and Shigella dysenteriae Type 1 by chitosan nanoparticle loaded with recombinant chimeric antigens comprising EIT and STX1B-IpaD., PMID:37704060
Repurposing the Pentameric B-Subunit of Shiga Toxin for Gb3-Targeted Immunotherapy of Colorectal Cancer by Rhamnose Conjugation., PMID:35905973
Lettuce-derived secretory IgA specifically neutralizes the Shiga toxin 1 activity., PMID:31222495
Secretion of the Shiga toxin B subunit (Stx1B) via an autotransporter protein optimizes the protective immune response to the antigen expressed in an attenuated E. coli (rEPEC E22Δler) vaccine strain., PMID:29102116
Novel fusion antigen displayed-bacterial ghosts vaccine candidate against infection of Escherichia coli O157:H7., PMID:26626573
Shiga toxin-induced apoptosis is more efficiently inhibited by dimeric recombinant hybrid-IgG/IgA immunoglobulins than by the parental IgG monoclonal antibodies., PMID:25469594
High-density genotyping of immune loci in Kawasaki disease and IVIG treatment response in European-American case-parent trio study., PMID:25101798
Stable expression and characterization of monomeric and dimeric recombinant hybrid-IgG/IgA immunoglobulins specific for Shiga toxin., PMID:24989136
A Poly-N-acetylglucosamine-Shiga toxin broad-spectrum conjugate vaccine for Shiga toxin-producing Escherichia coli., PMID:24667709
Production of hybrid-IgG/IgA plantibodies with neutralizing activity against Shiga toxin 1., PMID:24312238
Intranasal immunisation with Stx2B-Tir-Stx1B-Zot protein leads to decreased shedding in goats after challenge with Escherichia coli O157:H7., PMID:22186381
Establishment of recombinant hybrid-IgG/IgA immunoglobulin specific for Shiga toxin., PMID:21883352
Novel fusion protein protects against adherence and toxicity of enterohemorrhagic Escherichia coli O157:H7 in mice., PMID:21742003
Subcutaneous and intranasal immunization with Stx2B-Tir-Stx1B-Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice., PMID:21338683
Enhanced immunogenicity of a novel Stx2Am-Stx1B fusion protein in a mice model of enterohemorrhagic Escherichia coli O157:H7 infection., PMID:21134452
Monoclonal antibodies against Stx1B subunit of Escherichia coli O157:H7 distinguish the bacterium from other bacteria., PMID:20854398
Prokaryotic expression of Stx1B subunit of Escherichia coli O157:H7 used to generate monoclonal antibody., PMID:20715986
Immunogenicity of a novel Stx2B-Stx1B fusion protein in a mice model of Enterohemorrhagic Escherichia coli O157:H7 infection., PMID:19428832
Characterization of monoclonal immunoglobulin a and g against shiga toxin binding subunits produced by intranasal immunization., PMID:18782271
Immune responses of mice immunized with active recombinant shiga toxin and its derivatives., PMID:18552406
A nasal vaccine comprising B-subunit derivative of Shiga toxin 2 for cross-protection against Shiga toxin types 1 and 2., PMID:18387720
Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells., PMID:18253931
Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin., PMID:18093704
A comparison of Verotoxin B-subunit (Stx1B) and CD77 antibody to define germinal centre populations., PMID:16165112
Production of IgA monoclonal antibody against Shiga toxin binding subunits employing nasal-associated lymphoid tissue., PMID:15992815
Facilitated production of secretory IgA against Shiga toxin B subunits by intranasal application of antigen-coated polystyrene microspheres., PMID:15722600
Production of secretory immunoglobulin A against Shiga toxin-binding subunits in mice by mucosal immunization., PMID:14742533
Platelet activation by Shiga toxin and circulatory factors as a pathogenetic mechanism in the hemolytic uremic syndrome., PMID:11342436
For research use only. Not for human or drug use.
