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Anti-UBE2N Antibody (R1Z96)

Catalog #:   RHF40301 Specific References (9) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: IF, WB
Accession: P61088
Overview

Catalog No.

RHF40301

Species reactivity

Human, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

IF: 1:50-1:200, WB: 1:500-1:1000

Target

UbcH13, Bendless-like ubiquitin-conjugating enzyme, Ubiquitin-conjugating enzyme E2 N, Ubiquitin carrier protein N, BLU, Ubc13, E2 ubiquitin-conjugating enzyme N, Ubiquitin-protein ligase N, UBE2N

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P61088

Applications

IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1Z96

Data Image
  • Immunofluorescence
    Immunocytochemistry analysis of Ubiquitin Conjugating Enzyme E2 N (green) in Hela using Ubiquitin Conjugating Enzyme E2 N antibody,and DAPI(blue).
  • Western blot
    Western blot analysis of Ube2N/Ubc13 in HL-60, C6 lysates using Ube2N/Ubc13 antibody.
References

A tri-ionic anchor mechanism drives Ube2N-specific recruitment and K63-chain ubiquitination in TRIM ligases., PMID:31582740

In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6., PMID:31074699

Inhibition of Ubc13-mediated Ubiquitination by GPS2 Regulates Multiple Stages of B Cell Development., PMID:28039360

Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21., PMID:26150489

Human T-cell leukemia virus type 1 (HTLV-1) tax requires CADM1/TSLC1 for inactivation of the NF-κB inhibitor A20 and constitutive NF-κB signaling., PMID:25774694

BCL10 regulates RNF8/RNF168-mediated ubiquitination in the DNA damage response., PMID:24732096

Proteasome inhibition promotes Parkin-Ubc13 interaction and lysine 63-linked ubiquitination., PMID:24023840

Biological significance of structural differences between two highly conserved Ubc variants., PMID:19056346

Isolation of ubiquitin-E2 (ubiquitin-conjugating enzyme) complexes from erythroleukaemia cells using immunoaffinity techniques., PMID:11336652

Datasheet

Document Download

Anti-UBE2N Antibody (R1Z96).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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For research use only. Not for human or drug use.

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Anti-UBE2N Antibody (R1Z96) [RHF40301]
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