Catalog No.
RHF40302
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
UbcH13, Bendless-like ubiquitin-conjugating enzyme, Ubiquitin-conjugating enzyme E2 N, Ubiquitin carrier protein N, BLU, Ubc13, E2 ubiquitin-conjugating enzyme N, Ubiquitin-protein ligase N, UBE2N
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P61088
Applications
IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1Z95
A tri-ionic anchor mechanism drives Ube2N-specific recruitment and K63-chain ubiquitination in TRIM ligases., PMID:31582740
In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6., PMID:31074699
Inhibition of Ubc13-mediated Ubiquitination by GPS2 Regulates Multiple Stages of B Cell Development., PMID:28039360
Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21., PMID:26150489
Human T-cell leukemia virus type 1 (HTLV-1) tax requires CADM1/TSLC1 for inactivation of the NF-κB inhibitor A20 and constitutive NF-κB signaling., PMID:25774694
BCL10 regulates RNF8/RNF168-mediated ubiquitination in the DNA damage response., PMID:24732096
Proteasome inhibition promotes Parkin-Ubc13 interaction and lysine 63-linked ubiquitination., PMID:24023840
Biological significance of structural differences between two highly conserved Ubc variants., PMID:19056346
Isolation of ubiquitin-E2 (ubiquitin-conjugating enzyme) complexes from erythroleukaemia cells using immunoaffinity techniques., PMID:11336652