Catalog No.
RHE38601
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
Solute carrier family 1 member 3, EAAT1, Excitatory amino acid transporter 1, GLAST-1, SLC1A3, GLAST1, Sodium-dependent glutamate/aspartate transporter 1, GLAST
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P43003
Applications
IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1W35
Modulation of the IGF1R-MTOR pathway attenuates motor neuron toxicity of human ALS SOD1G93A astrocytes., PMID:33749521
Calcineurin Controls Expression of EAAT1/GLAST in Mouse and Human Cultured Astrocytes through Dynamic Regulation of Protein Synthesis and Degradation., PMID:32210081
GLAST (GLutamate and ASpartate Transporter) in human prefrontal cortex; interactome in healthy brains and the expression of GLAST in brains of chronic alcoholics., PMID:30817938
Quantification of brain-derived extracellular vesicles in plasma as a biomarker to diagnose Parkinson's and related diseases., PMID:30502924
Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis., PMID:30150703
Expression of Glutamate Transporters in Mouse Liver, Kidney, and Intestine., PMID:29303644
Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes., PMID:28317180
Blocking IL-17A Alleviates Diabetic Retinopathy in Rodents., PMID:28222445
Immunopathology: autoimmune glial diseases and differentiation from multiple sclerosis., PMID:27112673
Chondroitin sulfate proteoglycan tenascin-R regulates glutamate uptake by adult brain astrocytes., PMID:24337573
Expression of ALS-linked TDP-43 mutant in astrocytes causes non-cell-autonomous motor neuron death in rats., PMID:23714777
Changes in glutamate homeostasis cause retinal degeneration in Royal College of Surgeons rats., PMID:23483219
The rates of postmortem proteolysis of glutamate transporters differ dramatically between cells and between transporter subtypes., PMID:22859703
Overstimulation of NMDA receptors impairs early brain development in vivo., PMID:22606296
Isolation and characterization of living primary astroglial cells using the new GLAST-specific monoclonal antibody ACSA-1., PMID:22374709
Downregulation of glutamine synthetase via GLAST suppression induces retinal axonal swelling in a rat ex vivo hydrostatic pressure model., PMID:21775659
Regulation of glutamate transporter GLT-1 by MAGI-1., PMID:21426345
Intravitreal injection of exendin-4 analogue protects retinal cells in early diabetic rats., PMID:20688733
Origin, maturation, and astroglial transformation of secondary radial glial cells in the developing dentate gyrus., PMID:20549747
Distribution of the Na,K-ATPase alpha subunit in the rat spiral ganglion and organ of corti., PMID:19082858
Expression and distribution of 'high affinity' glutamate transporters GLT1, GLAST, EAAC1 and of GCPII in the rat peripheral nervous system., PMID:19014361
Reactive astrocytes and activated microglial cells express EAAT1, but not EAAT2, reflecting a neuroprotective potential following ischaemia., PMID:17543080
Expression of the exon 3 skipping form of GLAST, GLAST1a, in brain and retina., PMID:17179860
Regulation of glial development by cystatin C., PMID:17064358
The fate of neural progenitor cells expressing astrocytic and radial glial markers in the postnatal rat dentate gyrus., PMID:16262632
Development of GLAST(+) astrocytes and NG2(+) glia in rat hippocampus CA1: mature astrocytes are electrophysiologically passive., PMID:16093329
Induction of nestin, Ki-67, and cyclin D1 expression in Müller cells after laser injury in adult rat retina., PMID:15983812
Expression of glutamate transporter subtypes during normal human corticogenesis and type II lissencephaly., PMID:15804404
Differential regulation of GLAST immunoreactivity and activity by protein kinase C: evidence for modification of amino and carboxyl termini., PMID:15569258
Localization and expression of the glutamate transporter, excitatory amino acid transporter 4, within astrocytes of the rat retina., PMID:14727177
Neuronal glutamate transporter EAAT4 is expressed in astrocytes., PMID:12951653
Expression of glutamate transporters in human and rat retina and rat optic nerve., PMID:12898275
Decreased expression of glutamate transporters in genetic absence epilepsy rats before seizure occurrence., PMID:11953453
Nigrostriatal denervation does not affect glutamate transporter mRNA expression but subsequent levodopa treatment selectively increases GLT1 mRNA and protein expression in the rat striatum., PMID:11723182
Characterization of Na+-coupled glutamate/aspartate transport by a rat brain astrocyte line expressing GLAST and EAAC1., PMID:11426296
Comparative analysis of glutamate transporter expression in rat brain using differential double in situ hybridization., PMID:9683064
Neuronal expression of the glutamate transporter GLT-1 in hippocampal microcultures., PMID:9614226
The glutamate transporter EAAT4 in rat cerebellar Purkinje cells: a glutamate-gated chloride channel concentrated near the synapse in parts of the dendritic membrane facing astroglia., PMID:9570792
Structural selectivity and molecular nature of L-glutamate transport in cultured human fibroblasts., PMID:9606970
Glutamate transporter protein subtypes are expressed differentially during rat CNS development., PMID:9334410
Expression of high-affinity neuronal and glial glutamate transporters in the rat optic nerve., PMID:9215727
For research use only. Not for human or drug use.
