Catalog No.
RHD70101
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
Splicing factor U2AF 65 kDa subunit, hU2AF65, hU2AF(65), U2 auxiliary factor 65 kDa subunit, U2AF65, U2AF2, U2 snRNP auxiliary factor large subunit
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P26368
Applications
IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1T06
Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2., PMID:39495216
Splicing machinery is impaired in rheumatoid arthritis, associated with disease activity and modulated by anti-TNF therapy., PMID:34625402
Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome., PMID:25169209
Triplex DNA-binding proteins are associated with clinical outcomes revealed by proteomic measurements in patients with colorectal cancer., PMID:22682314
Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors., PMID:17137510
Substrate-dependent differences in U2AF requirement for splicing in adenovirus-infected cell extracts., PMID:15899895
Dual function for U2AF(35) in AG-dependent pre-mRNA splicing., PMID:11604503
The role of U2AF35 and U2AF65 in enhancer-dependent splicing., PMID:11421359
Serologic responses in patients with malignant mesothelioma: evidence for both public and private specificities., PMID:10783126
Evidence for substrate-specific requirement of the splicing factor U2AF(35) and for its function after polypyrimidine tract recognition by U2AF(65)., PMID:10567551
Pre-mRNA splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor., PMID:10049361
Existence and localization of a protein in zebra finch brain with similar structural features as the large subunit of the splicing factor U2AF., PMID:9597763
Targeting of U2AF65 to sites of active splicing in the nucleus., PMID:9166400
Novel nuclear autoantigen with splicing factor motifs identified with antibody from hepatocellular carcinoma., PMID:8227358
For research use only. Not for human or drug use.
