Catalog No.
RHD15009
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000
Target
H2a/x, Histone H2A.X, H2AFX, Histone H2AX, H2AX
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P16104
Applications
IF, IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1P92
Radiation-Induced Gamma-H2AX Foci Staining and Analysis., PMID:40418470
Detection of γ-H2A.X for Rapid Assessment of Genotoxic Agent-induced Double-strand DNA Breaks by Immunofluorescence., PMID:40257558
Hepatocytes and Hepatic Stellate Cells Carry Different Levels of DNA Damage due to Their Sensitivity to Oxidative Stress in Chronic Hepatitis B., PMID:39991880
Prediction of radiotherapy toxicity: 20 years of COPERNIC radiosensitivity diagnosis procedure., PMID:39307605
Sensitive Detection of Histones and γ-H2AX by Immunoblotting: Problems and Solutions., PMID:39237351
A multi-biomarker micronucleus assay using imaging flow cytometry., PMID:38995349
Preclinical activity of datopotamab deruxtecan, a novel TROP2 directed antibody-drug conjugate targeting trophoblast cell-surface antigen 2 (TROP2) in ovarian carcinoma., PMID:38981151
When Chromatin Decondensation Affects Nuclear γH2AX Foci Pattern and Kinetics and Biases the Assessment of DNA Double-Strand Breaks by Immunofluorescence., PMID:38927105
Multiplex imaging reveals spatially resolved DNA-damage response neighborhoods in TP53-mutated myelodysplastic neoplasms., PMID:38801208
Evidence for persistent UV-induced DNA damage and altered DNA damage response in xeroderma pigmentosa patient corneas., PMID:38641197
Pannexin 1 Modulates Angiogenic Activities of Human Endothelial Colony-Forming Cells Through IGF-1 Mechanism and Is a Marker of Senescence., PMID:37589139
Evidence of telomere attrition and a potential role for DNA damage in systemic sclerosis., PMID:35086525
Evaluation of crypt apoptotic bodies and apoptotic indices in pediatric celiac disease by routine staining and H2AX immunostaining., PMID:34137295
Targeting IGF Perturbs Global Replication through Ribonucleotide Reductase Dysfunction., PMID:33509941
Site-Specific Phosphorylation of Histone H1.4 Is Associated with Transcription Activation., PMID:33238524
Detection of DNA Double-Strand Breaks by γ-H2AX Immunodetection., PMID:32840768
CDK5 Activates Hippo Signaling to Confer Resistance to Radiation Therapy Via Upregulating TAZ in Lung Cancer., PMID:32407930
Human oocytes harboring damaged DNA can complete meiosis I., PMID:32276763
The Homologous Recombination Repair Pathway is Associated with Resistance to Radiotherapy in Nasopharyngeal Carcinoma., PMID:32015678
Biodosimetry of Low Dose Ionizing Radiation Using DNA Repair Foci in Human Lymphocytes., PMID:31947954
Investigating the level of DNA double-strand break in human spermatozoa and its relation to semen characteristics and IVF outcome using phospho-histone H2AX antibody as a biomarker., PMID:31393082
Preclinical Combination Studies of an FGFR2 Targeted Thorium-227 Conjugate and the ATR Inhibitor BAY 1895344., PMID:31255687
Detection of Histone H2AX Phosphorylation on Ser-139 as an Indicator of DNA Damage., PMID:31237414
Cell-free chromatin: A newly described mediator of systemic inflammation., PMID:31180045
Assessment of 213Bi-anti-EGFR MAb treatment efficacy in malignant cancer cells with [1-13C]pyruvate and [18F]FDG., PMID:31165773
Improved identification of DNA double strand breaks: γ-H2AX-epitope visualization by confocal microscopy and 3D reconstructed images., PMID:30799523
Identification and characterization of a novel nuclear structure containing members of the homologous recombination and DNA damage response pathways., PMID:30553479
Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies., PMID:30547460
Genome-wide mapping of 8-oxo-7,8-dihydro-2'-deoxyguanosine reveals accumulation of oxidatively-generated damage at DNA replication origins within transcribed long genes of mammalian cells., PMID:30462294
PARP inhibitors affect growth, survival and radiation susceptibility of human alveolar and embryonal rhabdomyosarcoma cell lines., PMID:30357520
An HER3-targeting antibody-drug conjugate incorporating a DNA topoisomerase I inhibitor U3-1402 conquers EGFR tyrosine kinase inhibitor-resistant NSCLC., PMID:30302022
DosiKit, a New Immunoassay for Fast Radiation Biodosimetry of Hair and Blood Samples., PMID:30074847
Using Persistent Homology as a New Approach for Super-Resolution Localization Microscopy Data Analysis and Classification of γH2AX Foci/Clusters., PMID:30072594
Cladribine in combination with entinostat synergistically elicits anti-proliferative/anti-survival effects on multiple myeloma cells., PMID:29969371
Genotoxic Effects in Human Fibroblasts Exposed to Microwave Radiation., PMID:29787439
Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation., PMID:29630045
Cell Type-Specific Quantification of Telomere Length and DNA Double-strand Breaks in Individual Lung Cells by Fluorescence In Situ Hybridization and Fluorescent Immunohistochemistry., PMID:29529386
Imaging of native transcription factors and histone phosphorylation at high resolution in live cells., PMID:29440513
Cytochrome c oxidase deficiency, oxidative stress, possible antioxidant therapy and link to nuclear DNA damage., PMID:29396562
Particles with similar LET values generate DNA breaks of different complexity and reparability: a high-resolution microscopy analysis of γH2AX/53BP1 foci., PMID:29271466
Low-Dose Hypersensitive Response for Residual pATM and γH2AX Foci in Normal Fibroblasts of Cancer Patients., PMID:29248168
Multiparameter cytometric analysis of complex cellular response., PMID:29220555
Immunofluorescence Analysis of γ-H2AX Foci in Mammalian Fibroblasts at Different Phases of the Cell Cycle., PMID:28710765
Enhanced radiation damage caused by iodinated contrast agents during CT examination., PMID:28624023
ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning Cytometry., PMID:28477120
Interlaboratory evaluation of a multiplexed high information content in vitro genotoxicity assay., PMID:28370322
Alpha-particle radiotherapy: For large solid tumors diffusion trumps targeting., PMID:28365545
Prostate cancer treated with brachytherapy; an exploratory study of dose-dependent biomarkers and quality of life., PMID:28288658
A comparison of radiation-induced mitochondrial damage between neural progenitor stem cells and differentiated cells., PMID:28118061
The comparative in vitro assessment of e-cigarette and cigarette smoke aerosols using the γH2AX assay and applied dose measurements., PMID:27965004
For research use only. Not for human or drug use.
