Catalog No.
RWC78001
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, WB: 1:500-1:1000
Target
GLI3 form of 190 kDa, GLI3FL, GLI3-83, Transcriptional activator GLI3, GLI3, GLI3-190, GLI3 C-terminally truncated form, GLI3 full-length protein, GLI3 form of 83 kDa
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q5IS56
Applications
IF, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1M55
Distinct Whole Transcriptomic Profiles of the Bursa of Fabricius in Muscovy Ducklings Infected by Novel Duck Reovirus with Different Virulence., PMID:36680150
New insights into the GDF9-Hedgehog-GLI signaling pathway in human ovaries: from fetus to postmenopause., PMID:33772413
The SHH/Gli axis regulates CD90-mediated liver cancer stem cell function by activating the IL6/JAK2 pathway., PMID:29722127
Centrosomal protein Dzip1l binds Cby, promotes ciliary bud formation, and acts redundantly with Bromi to regulate ciliogenesis in the mouse., PMID:29487109
PKA-mediated Gli2 and Gli3 phosphorylation is inhibited by Hedgehog signaling in cilia and reduced in Talpid3 mutant., PMID:28673820
Association of expression of the hedgehog signal with Merkel cell polyomavirus infection and prognosis of Merkel cell carcinoma., PMID:28551328
May Sonic Hedgehog proteins be markers for malignancy in uterine smooth muscle tumors?, PMID:26997437
Sonic Hedgehog regulates thymic epithelial cell differentiation., PMID:26778835
Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR., PMID:26179041
Hedgehog signaling stimulates the formation of proliferating Müller glia-derived progenitor cells in the chick retina., PMID:26116667
Sonic hedgehog signalling proteins are frequently expressed in retinoblastoma and are associated with aggressive clinicopathological features., PMID:25296932
Murine Joubert syndrome reveals Hedgehog signaling defects as a potential therapeutic target for nephronophthisis., PMID:24946806
Hedgehog signaling regulates telomerase reverse transcriptase in human cancer cells., PMID:24086482
Anti-apoptotic role of the sonic hedgehog signaling pathway in the proliferation of ameloblastoma., PMID:23835807
Overexpression of the Hedgehog signalling pathway in head and neck squamous cell carcinoma., PMID:23689223
GLI3 constrains digit number by controlling both progenitor proliferation and BMP-dependent exit to chondrogenesis., PMID:22465667
Abrogation of Gli3 expression suppresses the growth of colon cancer cells via activation of p53., PMID:22227409
MODY type 2 in Greig cephalopolysyndactyly syndrome (GCPS) as part of a contiguous gene deletion syndrome., PMID:22043488
Genome-wide RNAi screen reveals disease-associated genes that are common to Hedgehog and Wnt signaling., PMID:21266715
Ofd1 is required in limb bud patterning and endochondral bone development., PMID:20920500
Kinetics of hedgehog-dependent full-length Gli3 accumulation in primary cilia and subsequent degradation., PMID:20154143
Expression of hedgehog signaling molecules in Merkel cell carcinoma., PMID:19644931
Expression of sonic hedgehog signaling molecules in normal, hyperplastic and carcinomatous endometrium., PMID:19432668
Molecular specification and patterning of progenitor cells in the lateral and medial ganglionic eminences., PMID:18799682
Generation and characterization of a single-chain Fv antibody against G, a hedgehog signaling pathway transcription factor., PMID:18582209
Protein phosphatase 2A and rapamycin regulate the nuclear localization and activity of the transcription factor GLI3., PMID:18559511
Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3., PMID:17725385
Ucma--A novel secreted factor represents a highly specific marker for distal chondrocytes., PMID:17707622
Evc is a positive mediator of Ihh-regulated bone growth that localises at the base of chondrocyte cilia., PMID:17660199
Generation and characterization of mouse monoclonal antibody 5E1 against human transcription factor GLI3., PMID:17600494
A hypermorphic mouse Gli3 allele results in a polydactylous limb phenotype., PMID:17266131
Expression of homeotic genes Hoxa3, Hoxb3, Hoxd3 and Hoxc4 is decreased in the lungs but not in the hearts of adriamycin-exposed mice., PMID:17211587
Enhanced expression of hedgehog signaling molecules in squamous cell carcinoma of uterine cervix and its precursor lesions., PMID:16778829
Functional compensation in Hedgehog signaling during mouse prostate development., PMID:16707121
Increased apoptosis and accelerated epithelial migration following inhibition of hedgehog signaling in adaptive small bowel postresection., PMID:16439469
Hedgehog signaling pathway and gastric cancer., PMID:16258256
Inhibition of prostate cancer proliferation by interference with SONIC HEDGEHOG-GLI1 signaling., PMID:15314219
Sonic hedgehog expression and role in healing corneal epithelium., PMID:15277480
Sonic hedgehog signaling regulates Gli3 processing, mesenchymal proliferation, and differentiation during mouse lung organogenesis., PMID:15136151
Ski is involved in transcriptional regulation by the repressor and full-length forms of Gli3., PMID:12435627
Hedgehog-regulated processing of Gli3 produces an anterior/posterior repressor gradient in the developing vertebrate limb., PMID:10693759
Mouse suppressor of fused is a negative regulator of sonic hedgehog signaling and alters the subcellular distribution of Gli1., PMID:10531011
The Shh signalling pathway in early tooth development., PMID:10512189
Sonic hedgehog regulates the growth and patterning of the cerebellum., PMID:10375501
For research use only. Not for human or drug use.
