Catalog No.
RHB09101
Species reactivity
Human, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, WB: 1:500-1:1000
Target
ROCK2, KIAA0619, Rho-associated, coiled-coil-containing protein kinase 2, p164 ROCK-2, ROCK-II, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase II, Rho-associated protein kinase 2
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O75116
Applications
IF, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1F05
In Vitro Experiment Present ROCK2 Inhibition Promotes the Therapeutic Effect of Bevacizumab in the Treatment of Glioblastoma Multiforme., PMID:39792535
The ROCK-1/2 inhibitor RKI-1447 blocks N-MYC, promotes cell death, and emerges as a synergistic partner for BET inhibitors in neuroblastoma., PMID:39307412
Augmentation of functional recovery via ROCK/PI3K/AKT pathway by Fasudil Hydrochloride in a rat sciatic nerve transection model., PMID:39007038
Modulating asthma-COPD overlap responses with IL-17 inhibition., PMID:37965351
Philadelphia chromosome-negative myeloproliferative chronic neoplasms: is clonal hematopoiesis the main determinant of autoimmune and cardio-vascular manifestations?, PMID:37915324
Involvement of Paired Immunoglobulin-Like Receptor B in Cognitive Dysfunction Through Hippocampal-Dependent Synaptic Plasticity Impairments in Mice Subjected to Chronic Sleep Restriction., PMID:36417104
Inhibition of Nogo-A rescues synaptic plasticity and associativity in APP/PS1 animal model of Alzheimer's disease., PMID:35431138
ROCK2 inhibition attenuates profibrogenic immune cell function to reverse thioacetamide-induced liver fibrosis., PMID:34917911
Critical regulation of follicular helper T cell differentiation and function by Gα13 signaling., PMID:34663730
Silencing ROCK1 ameliorates ventilator-induced lung injury in mice by inhibiting macrophages' NLRP3 signaling., PMID:34619496
Activin A and Cell-Surface GRP78 Are Novel Targetable RhoA Activators for Diabetic Kidney Disease., PMID:33799579
Effect of anti-IL17 and/or Rho-kinase inhibitor treatments on vascular remodeling induced by chronic allergic pulmonary inflammation., PMID:33357114
Profilin 1 knockdown prevents ischemic brain damage by promoting M2 microglial polarization associated with the RhoA/ROCK pathway., PMID:32291804
Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF-Resistant Diabetic Macular Edema., PMID:32139595
Fibronectin regulates the self-renewal of rabbit limbal epithelial stem cells by stimulating the Wnt11/Fzd7/ROCK non-canonical Wnt pathway., PMID:31150636
Immunoglobulin Binding Protein 1 as a Potential Urine Biomarker in Patients with Lupus Nephritis., PMID:31137925
Loss of ARHGEF1 causes a human primary antibody deficiency., PMID:30521495
Effect of Anti-IL17 Antibody Treatment Alone and in Combination With Rho-Kinase Inhibitor in a Murine Model of Asthma., PMID:30233389
The CCM1-CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity., PMID:30030370
Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats., PMID:29749502
Discovery of Potent and Selective MRCK Inhibitors with Therapeutic Effect on Skin Cancer., PMID:29382705
ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy., PMID:28821742
Genetic Interference With Endothelial PPAR-γ (Peroxisome Proliferator-Activated Receptor-γ) Augments Effects of Angiotensin II While Impairing Responses to Angiotensin 1-7., PMID:28674038
Cytokine mediators of chronic graft-versus-host disease., PMID:28665299
Rho-associated protein kinase 2 (ROCK2): a new target of autoimmunity in paraneoplastic encephalitis., PMID:28554330
Inhibition of lysophosphatidic acid receptor ameliorates Sjögren's syndrome in NOD mice., PMID:28460477
The Role of Rho/Rho-Kinase Pathway in the Pathogenesis of Cholesteatoma., PMID:28288476
RhoA/ROCK pathway inhibition by fasudil suppresses the vasculogenic mimicry of U2OS osteosarcoma cells in vitro., PMID:28225457
Targeting ROCK2 rather than ROCK1 inhibits Ewing sarcoma malignancy., PMID:28112365
ROCK2 signaling is required to induce a subset of T follicular helper cells through opposing effects on STATs in autoimmune settings., PMID:27436361
Attenuation of obesity-induced insulin resistance in mice with heterozygous deletion of ROCK2., PMID:27163743
Targeted Rho-associated kinase 2 inhibition suppresses murine and human chronic GVHD through a Stat3-dependent mechanism., PMID:26983850
ROCK inhibition with fasudil promotes early functional recovery of spinal cord injury in rats by enhancing microglia phagocytosis., PMID:26838736
In vivo expression and regulation of genes associated with vascularization during early response of sutures to tensile force., PMID:26825658
Immunohistochemical evaluation of ROCK activation in invasive breast cancer., PMID:26626121
Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis., PMID:26536659
Examination of segmental average mass spectra from liquid chromatography-tandem mass spectrometric (LC-MS/MS) data enables screening of multiple types of protein modifications., PMID:26388481
Rotenone inhibits primary murine myotube formation via Raf-1 and ROCK2., PMID:25827955
Functional relationship among PLK2, PLK4 and ROCK2 to induce centrosome amplification., PMID:25590559
In vivo screening for substrates of protein kinase A using a combination of proteomic approaches and pharmacological modulation of kinase activity., PMID:25399539
Abrogation of airway hyperresponsiveness but not inflammation by rho kinase insufficiency., PMID:25323425
Upregulation of Gem relates to retinal ganglion cells apoptosis after optic nerve crush in adult rats., PMID:24948002
Prostaglandin E(2)-dependent blockade of actomyosin and stress fibre formation is mediated through S1379 phosphorylation of ROCK2., PMID:24610576
Ser1333 phosphorylation indicates ROCKI activation., PMID:24168723
Anti-β2GPI antibodies stimulate endothelial cell microparticle release via a nonmuscle myosin II motor protein-dependent pathway., PMID:23954892
CD99 suppresses osteosarcoma cell migration through inhibition of ROCK2 activity., PMID:23644663
Distinct distribution and localization of Rho-kinase in mouse epithelial, muscle and neural tissues., PMID:22986902
The effects of erythropoietin on RhoA/Rho-associated kinase expression in rat retinal explants cultured with glutamate., PMID:22711191
Elevated levels of RhoA in the optic nerve head of human eyes with glaucoma., PMID:22495072
In vivo evidence that TRAF4 is required for central nervous system myelin homeostasis., PMID:22363515
For research use only. Not for human or drug use.
