Catalog No.
                        RHA94903
                        
                                            
                                            
                                            
                                            
                                            
                                            
                                            
                                            Species reactivity
                        
                        Human                        
                                                
                                            Host species
                        
                        Rabbit                        
                                                
                                            Isotype
                        
                        IgG                        
                                                
                                            Clonality
                        
                        Monoclonal                        
                                                
                                            
                                            
                                            Tested applications
                        
                        IF: 1:50-1:200, IP: 1:20, WB: 1:500-1:1000                        
                                                
                                            Target
                        
                        SSK1, MAD3/BUB1-related protein kinase, BUB1B, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, BUBR1, MAD3L, Protein SSK1, hBUBR1, Mitotic checkpoint kinase MAD3L                        
                                                
                                            
                                            Concentration
                        
                        1 mg/ml                        
                                                
                                            Endotoxin level
                        
                        Please contact with the lab for this information.                        
                                                
                                            Purity
                        
                        >95% by SDS-PAGE.                        
                                                
                                            Purification
                        
                        Protein A/G purified from cell culture supernatant.                        
                                                
                                            Accession
                        
                        O60566                        
                                                
                                            
                                            Applications
                        
                        IF, IP, WB                        
                                                
                                            Form
                        
                        Liquid                        
                                                
                                            Storage buffer
                        
                        0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.                        
                                                
                                            
                                            
                                            
                                            
                                            
                                            
                                            
                                            
                                            
                                            
                                            
                                            Stability and Storage
                        
                        Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.                        
                                                
                                            
                                            
                                                Clone ID
                            R1E69
                            
                                                    
                                            
                                            
                                        
                 
                
                
                                            Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes., PMID:26028219
                                            Deregulation of HMGA1 expression induces chromosome instability through regulation of spindle assembly checkpoint genes., PMID:26009897
                                            SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation., PMID:25123474
                                            Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes., PMID:22513372
                                            BRCA2 fine-tunes the spindle assembly checkpoint through reinforcement of BubR1 acetylation., PMID:22340495
                                            MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes., PMID:20406800
                                            BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment., PMID:17709426
                                            BubR1 is involved in regulation of DNA damage responses., PMID:16449973
                                            Monoallelic BUB1B mutations and defective mitotic-spindle checkpoint in seven families with premature chromatid separation (PCS) syndrome., PMID:16411201
                                            Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteins., PMID:12235289