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Anti-BUB1B Antibody (R1E68)

Catalog #:   RHA94902 Specific References (10) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: FCM, IHC, IP, WB
Accession: O60566
Overview

Catalog No.

RHA94902

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Tested applications

FCM: 1:20-1:100, IHC: 1:100-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000

Target

SSK1, MAD3/BUB1-related protein kinase, BUB1B, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, BUBR1, MAD3L, Protein SSK1, hBUBR1, Mitotic checkpoint kinase MAD3L

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O60566

Applications

FCM, IHC, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.

Clone ID

R1E68

Data Image
  • Western blot
    Western blot analysis of BubR1 expression in HepG2 cell lysate.
  • Western blot
    All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
References

Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes., PMID:26028219

Deregulation of HMGA1 expression induces chromosome instability through regulation of spindle assembly checkpoint genes., PMID:26009897

SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation., PMID:25123474

Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes., PMID:22513372

BRCA2 fine-tunes the spindle assembly checkpoint through reinforcement of BubR1 acetylation., PMID:22340495

MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes., PMID:20406800

BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment., PMID:17709426

BubR1 is involved in regulation of DNA damage responses., PMID:16449973

Monoallelic BUB1B mutations and defective mitotic-spindle checkpoint in seven families with premature chromatid separation (PCS) syndrome., PMID:16411201

Anaphase onset does not require the microtubule-dependent depletion of kinetochore and centromere-binding proteins., PMID:12235289

Datasheet

Document Download

Anti-BUB1B Antibody (R1E68).pdf

 

$ 290
Product specifications
50 μg 290 100 μg 484

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For research use only. Not for human or drug use.

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Anti-BUB1B Antibody (R1E68) [RHA94902]
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