Catalog No.
RHA81801
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
KAR-associated protein, DAP12, TYROBP, KARAP, TYRO protein tyrosine kinase-binding protein, Killer-activating receptor-associated protein, DNAX-activation protein 12
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O43914
Applications
FCM, IF, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1E42
Transcriptomic signatures in peripheral CD4+T-lymphocytes may reflect melanoma staging and immunotherapy responsiveness prior to ICI initiation., PMID:40226614
Integrated bioinformatic analysis of the shared molecular mechanisms between ANCA-associated vasculitis and atherosclerosis., PMID:39702436
Novel Therapies for ANCA-associated Vasculitis: Apilimod Ameliorated Endothelial Cells Injury through TLR4/NF-κB Pathway and NLRP3 Inflammasome., PMID:38910483
Neoadjuvant camrelizumab (an anti-PD-1 antibody) plus chemotherapy or apatinib (a VEGFR-2 inhibitor) for initially unresectable stage II-III non-small-cell lung cancer: a multicentre, two-arm, phase 2 exploratory study., PMID:38871690
Identification of Key Biomarkers and Immune Infiltration in Liver Tissue after Bariatric Surgery., PMID:35789605
Identification of differentially expressed genes and pathways in kidney of ANCA-associated vasculitis by integrated bioinformatics analysis., PMID:35172670
Reporter cell assay for human CD33 validated by specific antibodies and human iPSC-derived microglia., PMID:34188106
A reporter cell system for the triggering receptor expressed on myeloid cells 2 reveals differential effects of disease-associated variants on receptor signaling and activation by antibodies against the stalk region., PMID:33314333
A Humanized Lym-1 CAR with Novel DAP10/DAP12 Signaling Domains Demonstrates Reduced Tonic Signaling and Increased Antitumor Activity in B-Cell Lymphoma Models., PMID:32273277
Natural receptor-based competitive immunoelectrochemical assay for ultra-sensitive detection of Siglec 15., PMID:31868605
The prognostic value of JUNB-positive CTCs in metastatic breast cancer: from bioinformatics to phenotypic characterization., PMID:31370904
TREM1/Dap12-based CAR-T cells show potent antitumor activity., PMID:31268375
Curcumin restores innate immune Alzheimer's disease risk gene expression to ameliorate Alzheimer pathogenesis., PMID:30951849
Targeting of TAM Receptors Ameliorates Fibrotic Mechanisms in Idiopathic Pulmonary Fibrosis., PMID:29634284
TREM-1 multimerization is essential for its activation on monocytes and neutrophils., PMID:29568119
NKG2H-Expressing T Cells Negatively Regulate Immune Responses., PMID:29545803
Triggering receptor expressed on myeloid cells 2 (TREM2) dependent microglial activation promotes cisplatin-induced peripheral neuropathy in mice., PMID:29051087
A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells., PMID:28490631
TREM2/DAP12 Signal Elicits Proinflammatory Response in Microglia and Exacerbates Neuropathic Pain., PMID:27798193
Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms., PMID:25993291
Generation of Potent T-cell Immunotherapy for Cancer Using DAP12-Based, Multichain, Chimeric Immunoreceptors., PMID:25941351
Siglec-15 is a potential therapeutic target for postmenopausal osteoporosis., PMID:25460183
The C-type lectin OCILRP2 costimulates EL4 T cell activation via the DAP12-Raf-MAP kinase pathway., PMID:25411776
Mechanism and function of monoclonal antibodies targeting siglec-15 for therapeutic inhibition of osteoclastic bone resorption., PMID:24446437
Loss of DAP12 and FcRγ drives exaggerated IL-12 production and CD8(+) T cell response by CCR2(+) Mo-DCs., PMID:24155889
Siglec-15 regulates osteoclast differentiation by modulating RANKL-induced phosphatidylinositol 3-kinase/Akt and Erk pathways in association with signaling Adaptor DAP12., PMID:23677868
The interaction between Siglec-15 and tumor-associated sialyl-Tn antigen enhances TGF-β secretion from monocytes/macrophages through the DAP12-Syk pathway., PMID:23035012
CLEC5A regulates Japanese encephalitis virus-induced neuroinflammation and lethality., PMID:22536153
Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice., PMID:22005300
The immunoreceptor adapter protein DAP12 suppresses B lymphocyte-driven adaptive immune responses., PMID:21727189
DAP12 is required for macrophage recruitment to the lung in response to cigarette smoke and chemotaxis toward CCL2., PMID:20421649
Myeloid DAP12-associating lectin (MDL)-1 regulates synovial inflammation and bone erosion associated with autoimmune arthritis., PMID:20212065
Pattern of DAP12 expression in leukocytes from both healthy and systemic lupus erythematosus patients., PMID:19606219
Cross-regulation of signaling by ITAM-associated receptors., PMID:19295630
TREM-2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells., PMID:19017976
Developmental neuronal death in hippocampus requires the microglial CD11b integrin and DAP12 immunoreceptor., PMID:18685038
Evidence that the KIR2DS5 gene codes for a surface receptor triggering natural killer cell function., PMID:18624290
CLEC5A is critical for dengue-virus-induced lethal disease., PMID:18496526
PDC-TREM, a plasmacytoid dendritic cell-specific receptor, is responsible for augmented production of type I interferon., PMID:18287072
Sustained NKG2D engagement induces cross-tolerance of multiple distinct NK cell activation pathways., PMID:18198346
The TREM-1/DAP12 pathway., PMID:18192027
Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner., PMID:17982101
Siglec-15: an immune system Siglec conserved throughout vertebrate evolution., PMID:17483134
Allelic expression patterns of KIR3DS1 and 3DL1 using the Z27 and DX9 antibodies., PMID:17301953
Negative regulation of leucocyte functions by CD33-related siglecs., PMID:17073742
Regulation of Ly49D/DAP12 signal transduction by Src-family kinases and CD45., PMID:16709819
Inhibition of immune responses by ITAM-bearing receptors., PMID:16449667
TREM2, a DAP12-associated receptor, regulates osteoclast differentiation and function., PMID:16418779
Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12., PMID:16293595
Convergence on a distinctive assembly mechanism by unrelated families of activating immune receptors., PMID:15845448
For research use only. Not for human or drug use.
