Catalog No.
RHA74901
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000
Target
Mitotic checkpoint protein BUB3, BUB3
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O43684
Applications
FCM, IF, IHC, IP, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1E28
The closed form of Mad2 is bound to Mad1 and Cdc20 at unattached kinetochores., PMID:29895228
Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling., PMID:26986935
Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation., PMID:26399325
Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing., PMID:25918225
BuGZ is required for Bub3 stability, Bub1 kinetochore function, and chromosome alignment., PMID:24462187
Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction., PMID:22331848
Effects of artesunate on cytokinesis and G₂/M cell cycle progression of tumour cells and budding yeast., PMID:21156967
Benzo[a]pyrene treatment leads to changes in nuclear protein expression and alternative splicing., PMID:20097212
The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly., PMID:16807356
The Drosophila Bub3 protein is required for the mitotic checkpoint and for normal accumulation of cyclins during G2 and early stages of mitosis., PMID:15615783
The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1., PMID:15556864
Different spindle checkpoint proteins monitor microtubule attachment and tension at kinetochores in Drosophila cells., PMID:15075237
Analysis of Bub3 spindle checkpoint function in Xenopus egg extracts., PMID:12538762
The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity., PMID:12514103
Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores., PMID:11451998
Spindle checkpoint protein Bub1 is required for kinetochore localization of Mad1, Mad2, Bub3, and CENP-E, independently of its kinase activity., PMID:11402067
Human centromeres and neocentromeres show identical distribution patterns of >20 functionally important kinetochore-associated proteins., PMID:10607828
Retention of the BUB3 checkpoint protein on lagging chromosomes., PMID:10411903
Localization of the Drosophila checkpoint control protein Bub3 to the kinetochore requires Bub1 but not Zw10 or Rod., PMID:9914369
Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast., PMID:7593191
Suppression of a conditional mutation in alpha-tubulin by overexpression of two checkpoint genes., PMID:7622604
For research use only. Not for human or drug use.
