Catalog No.
RHA73401
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IF: 1:50-1:200, WB: 1:500-1:1000
Target
Nck-2, Cytoplasmic protein NCK2, NCK adaptor protein 2, GRB4, Growth factor receptor-bound protein 4, SH2/SH3 adaptor protein NCK-beta, NCK2
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
O43639
Applications
IF, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 ℃ for frequent use. Store at -20 ℃ for twelve months from the date of receipt.
Clone ID
R1E24
Nck adapter proteins promote podosome biogenesis facilitating extracellular matrix degradation and cancer invasion., PMID:31638742
A Study of Interleukin-2 Production and Nck Adaptor Molecule Gene Expression in Systemic Lupus Erythematosus Patients., PMID:29911835
Renal microRNA- and RNA-profiles in progressive chronic kidney disease., PMID:26707063
Nck influences preosteoblastic/osteoblastic migration and bone mass., PMID:26621720
Evidence for inducible recruitment of Wiskott-Aldrich syndrome protein to T cell receptor-CD3 complex in Jurkat T cells., PMID:26342115
Targeting of the EGFR/β1 integrin connecting proteins PINCH1 and Nck2 radiosensitizes three-dimensional SCC cell cultures., PMID:26004008
Depletion of the adaptor protein NCK increases UV-induced p53 phosphorylation and promotes apoptosis., PMID:24086708
The adaptor molecule Nck localizes the WAVE complex to promote actin polymerization during CEACAM3-mediated phagocytosis of bacteria., PMID:22448228
Nck adaptor proteins link Tks5 to invadopodia actin regulation and ECM degradation., PMID:19596797
Signalling via integrins: implications for cell survival and anticancer strategies., PMID:17084981
Brain-derived neurotrophic factor promotes interaction of the Nck2 adaptor protein with the TrkB tyrosine kinase receptor., PMID:12074588
Nckbeta adapter regulates actin polymerization in NIH 3T3 fibroblasts in response to platelet-derived growth factor bb., PMID:11027258
Nck-2, a novel Src homology2/3-containing adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways., PMID:9843575
For research use only. Not for human or drug use.
