Catalog No.
RHJ23601
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Monoclonal
Tested applications
IHC: 1:100-1:200, WB: 1:1000-1:2000
Target
Mixed lineage kinase domain-like protein, hMLKL, MLKL
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
Q8NB16
Applications
IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium azide.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Store at +4 ℃ short term (1-2 weeks).
Store at -20 ℃ 12 months.
Store at -80 ℃ long term.
Clone ID
R1C16
Ficolin-3 Activates Complement and Triggers Necroptosis in Cholangiocarcinoma Cells via the RIPK1/RIPK3/MLKL Signaling Pathway., PMID:40407231
MLKL-Mediated Necroptosis Predominantly Contributes to Immune-Associated Myocardial Damage., PMID:40195182
A determination of the main regulators of necroptosis in testicular tissue under different heat stresses., PMID:39856359
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells., PMID:39633178
Serum HMGB-1 released by ferroptosis and necroptosis as a novel potential biomarker for systemic lupus erythematosus., PMID:39128419
Mechanisms of programmed cell death associated to severe dengue in human renal lesions., PMID:39025381
Metabolic changes enhance necroptosis of type 2 diabetes mellitus mice infected with Mycobacterium tuberculosis., PMID:38728367
PANoptosis Features, a Humanized NSG Murine Model of Sjogren's Syndrome., PMID:38635961
Pharmacological inhibition of ICOS attenuates the protective effect of exercise on cardiac fibrosis induced by isoproterenol., PMID:38224847
The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells., PMID:37995810
Activation of the TNF-α-Necroptosis Pathway in Parvalbumin-Expressing Interneurons of the Anterior Cingulate Cortex Contributes to Neuropathic Pain., PMID:37895135
Vagus Nerve Stimulation Prevents Endothelial Necroptosis to Alleviate Blood-Spinal Cord Barrier Disruption After Spinal Cord Injury., PMID:37460917
Major adverse cardiovascular events are associated with necroptosis during severe COVID-19., PMID:37081485
SARS-CoV-2 immune complex triggers human monocyte necroptosis., PMID:36870284
Necroptosis Drives Major Adverse Cardiovascular Events During Severe COVID-19., PMID:36711834
Cannabinoid Receptor 2-Centric Molecular Feedback Loop Drives Necroptosis in Diabetic Heart Injuries., PMID:36448459
Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells., PMID:36342164
Necroptosis Plays a Crucial Role in Vascular Injury during DVT and Is Enhanced by IL-17B., PMID:36046722
The HSP90 inhibitor 17-DMAG alleviates primary biliary cholangitis via cholangiocyte necroptosis prevention., PMID:36037317
Preparation of the RIPK3 Polyclonal Antibody and Its Application in Immunoassays of Nephropathogenic Infectious Bronchitis Virus-Infected Chickens., PMID:36016369
Diverse actions of sirtuin-1 on ovulatory genes and cell death pathways in human granulosa cells., PMID:35840944
An IgM monoclonal antibody against domain 1 of CD147 induces non-canonical RIPK-independent necroptosis in a cell type specific manner in hepatocellular carcinoma cells., PMID:35598753
Necroptotic TNFα-Syndecan 4-TNFα Vicious Cycle as a Therapeutic Target for Preventing Temporomandibular Joint Osteoarthritis., PMID:35278225
Kupffer cells play a crucial role in monocrotaline-induced liver injury by producing TNF-α., PMID:35065160
TNF-α-dependent neuronal necroptosis regulated in Alzheimer's disease by coordination of RIPK1-p62 complex with autophagic UVRAG., PMID:34646380
Alendronate Augments Lipid A-Induced IL-1α Release via Activation of ASC but Not Caspase-11., PMID:34080091
Airway epithelial cell necroptosis contributes to asthma exacerbation in a mouse model of house dust mite-induced allergic inflammation., PMID:34045680
Boosting mitochondria activity by silencing MCJ overcomes cholestasis-induced liver injury., PMID:33997750
Cell death of hippocampal CA1 astrocytes during early epileptogenesis., PMID:33955001
MLKL inhibits intestinal tumorigenesis by suppressing STAT3 signaling pathway., PMID:33767595
A toolbox for imaging RIPK1, RIPK3, and MLKL in mouse and human cells., PMID:33589776
The necroptotic cell death pathway operates in megakaryocytes, but not in platelet synthesis., PMID:33510145
Detection of necroptosis by phospho-MLKL immunohistochemical labeling., PMID:33458710
Inducing regulated necrosis and shifting macrophage polarization with anti-EMMPRIN antibody (161-pAb) and complement factors., PMID:33205451
Increased MLKL mRNA level in the PBMCs is correlated with autoantibody production, renal involvement, and SLE disease activity., PMID:33054864
Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production., PMID:32595496
Protein-Bound Polysaccharides from Coriolus Versicolor Induce RIPK1/RIPK3/MLKL-Mediated Necroptosis in ER-Positive Breast Cancer and Amelanotic Melanoma Cells., PMID:32531147
Squalene emulsion-based vaccine adjuvants stimulate CD8 T cell, but not antibody responses, through a RIPK3-dependent pathway., PMID:32515732
Caspase-8-Dependent Inflammatory Responses Are Controlled by Its Adaptor, FADD, and Necroptosis., PMID:32428502
Endoplasmic reticulum stress occurs in association with the extrusion of toxic extracellular vesicles from human placentae treated with antiphospholipid antibodies., PMID:32068238
Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages., PMID:32051018
HMGB1-associated necroptosis and Kupffer cells M1 polarization underlies remote liver injury induced by intestinal ischemia/reperfusion in rats., PMID:31961020
Necroptotic cell binding of β2 -glycoprotein I provides a potential autoantigenic stimulus in systemic lupus erythematosus., PMID:31187539
Pathogenesis of lupus nephritis: RIP3 dependent necroptosis and NLRP3 inflammasome activation., PMID:31133359
FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation., PMID:31028177
Complement C5b-9 and Cancer: Mechanisms of Cell Damage, Cancer Counteractions, and Approaches for Intervention., PMID:31024572
Necroptotic Cell Death Promotes Adaptive Immunity Against Colonizing Pneumococci., PMID:31019504
RIP1 kinase inhibitor halts the progression of an immune-induced demyelination disease at the stage of monocyte elevation., PMID:30837313
The pathological features of regulated necrosis., PMID:30714148
Stimulations of the Culture Medium of Activated Microglia and TNF-Alpha on a Scrapie-Infected Cell Line Decrease the Cell Viability and Induce Marked Necroptosis That Also Occurs in the Brains from the Patients of Human Prion Diseases., PMID:30399321
For research use only. Not for human or drug use.
