Catalog No.
KAV00309
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant RBD (JN.1) has been pre-coated onto a microplate. Standards or samples are premixed with HRP labeled ACE and then pipetted into the wells. Anti-RBD (JN.1) Neutralizing Antibody in the sample competitively binds to recombinant RBD (JN.1) with the HRP labeled ACE. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Anti-RBD (JN.1) Neutralizing Antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
SARS-CoV-2 Surrogate Virus Neutralization Antibody (JN.1)
Applications
Used for the quantitative determination of Anti-RBD (JN.1) Neutralizing Antibody concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
175.58 - 2,000 ng/mL
Sensitivity
175.05 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <20%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <20%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
1797.0 |
958.5 |
497.4 |
1836.6 |
984.7 |
528.7 |
|
Standard deviation |
58.9 |
71.7 |
29.1 |
68.8 |
63.3 |
58.8 |
|
CV (%) |
3.3 |
7.5 |
5.9 |
3.7 |
6.4 |
11.1 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
For research use only. Not for human or drug use.
