Catalog No.
KAK24001
Description
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Ds DNA has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-dsDNA Mouse IgG present is bound by the immobilized protein. After washing away any unbound substances, a HRP-labeled Goat Anti-Mouse IgG antibody is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-dsDNA Mouse IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
Anti-dsDNA Mouse IgG Antibody
Applications
Used for the quantitative determination of Anti-dsDNA Mouse IgG concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
1.56 - 100 ng/mL
Sensitivity
0.43 ng/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision |
Inter-Assay Precision |
||||
|
Sample |
1 |
2 |
3 |
1 |
2 |
3 |
|
n |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean (ng/mL) |
51.6 |
13.4 |
2.7 |
44.5 |
11.7 |
2.6 |
|
Standard deviation |
4.9 |
1.1 |
0.2 |
2.9 |
1.0 |
0.3 |
|
CV (%) |
9.5 |
7.8 |
6.3 |
6.6 |
8.5 |
9.9 |
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
For research use only. Not for human or drug use.
