Catalog No.
KVV13402
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Monkeypox Virus A29L has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Monkeypox Virus A29L Antigen present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Monkeypox Virus A29L is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Monkeypox Virus A29L Antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.
Specificity
MPXV A29L
Applications
Used for the quantitative determination of Monkeypox Virus A29L Antigen concentration in serum and plasma.
Detection method
Colorimetric
Sample type
Plasma, Serum
Assay type
Quantitative
Range
7.81 - 500 pg/mL
Sensitivity
3.93 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
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1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (pg/mL)
|
200.9
|
48.0
|
10.9
|
218.0
|
51.3
|
12.5
|
|
Standard deviation
|
18.4
|
4.2
|
0.5
|
23.0
|
3.8
|
1.6
|
|
CV (%)
|
9.2
|
8.7
|
4.5
|
10.5
|
7.3
|
13.2
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
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A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates., PMID:39639037
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Monkeypox virus protein H3L induces injuries in human and mouse., PMID:39168969
A clinically used anti-human papilloma virus agent (3-hydroxyphthalic anhydride-modified bovine β-lactoglobulin) has a potential for topical application to prevent sexual transmission of monkeypox virus., PMID:39105195
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Polyvalent mpox mRNA vaccines elicit robust immune responses and confer potent protection against vaccinia virus., PMID:38787725
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Rapid identification of A29L antibodies based on mRNA immunization and high-throughput single B cell sequencing to detect Monkeypox virus., PMID:38517731
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Fluorescence-enhanced dual signal lateral flow immunoassay for flexible and ultrasensitive detection of monkeypox virus., PMID:38001482
Development and validation of a highly specific in-house chemiluminescent-based serological assay for the detection of antibodies directed against the human monkeypox virus., PMID:37890729
Evaluation and comparison of immune responses induced by two Mpox mRNA vaccine candidates in mice., PMID:37800627
Cross-reactive antibody response to Monkeypox virus surface proteins in a small proportion of individuals with and without Chinese smallpox vaccination history., PMID:37784185
Duration of humoral immunity from smallpox vaccination and its cross-reaction with Mpox virus., PMID:37709783
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Recombinant proteins A29L, M1R, A35R, and B6R vaccination protects mice from mpox virus challenge., PMID:37435062
Mpox virus mRNA-lipid nanoparticle vaccine candidates evoke antibody responses and drive protection against the Vaccinia virus challenge in mice., PMID:37429529
Immunogenic proteins and potential delivery platforms for mpox virus vaccine development: A rapid review., PMID:37353117
Multi-valent mRNA vaccines against monkeypox enveloped or mature viron surface antigens demonstrate robust immune response and neutralizing activity., PMID:37300753
Three neutralizing mAbs induced by MPXV A29L protein recognizing different epitopes act synergistically against orthopoxvirus., PMID:37288876
Design peptide and multi-epitope protein vaccine candidates against monkeypox virus using reverse vaccinology approach: an in-silico study., PMID:37154825
Monkeypox virus quadrivalent mRNA vaccine induces immune response and protects against vaccinia virus., PMID:37117161
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BoHV-4-Based Vector Single Heterologous Antigen Delivery Protects STAT1(-/-) Mice from Monkeypoxvirus Lethal Challenge., PMID:26086739
For research use only. Not for human or drug use.
