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Anti-ASFV pM1249L Polyclonal Antibody

Catalog #:   PVV38701 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q65152
Overview

Catalog No.

PVV38701

Species reactivity

African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant ASFV pM1249L (Gln346-Pro452).

Tested applications

ELISA: 1:4000-1:8000, WB: 1:1000-1:4000

Target

Minor capsid protein M1249L, pM1249L, Ba71V-060, M1249L

Purification

Purified by antigen affinity column.

Accession

Q65152

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with pM1249L antibody (PVV38701) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 15 kDa
    Observed MW: 15 kDa
References

Selection, Design, and Immunogenicity Studies of ASFV Antigens for Subunit mRNA Cocktail Vaccines with Specific Immune Response Profiles., PMID:40478837

A phage-displayed nanobody-based competitive immunoassay for the detection of African swine fever virus antibodies., PMID:40474190

Identification of a linear B-cell epitope in B117L-213 protein of ASFV using monoclonal antibodies., PMID:40456193

Anti-pA137R antibodies exacerbate the pathogenicity of African swine fever virus in pigs., PMID:40439408

First study to describe the prevalence and epidemiology of African swine fever, classical swine fever, porcine reproductive and respiratory syndrome and swine flu in Kazakhstan., PMID:40437487

The Novel Antigenic Epitopes of African Swine Fever Virus Inner Membrane p54 Protein Revealed by Monoclonal Antibodies., PMID:40362108

Encapsulin nanoparticle-conjugated p54 protein boosts immune responses against African swine fever virus., PMID:40324494

Establishment of an immunoperoxidase monolayer assay for the detection of African swine fever virus antibodies., PMID:40320012

Preparation and epitope mapping of monoclonal antibodies against African swine fever virus A238L protein., PMID:40311975

Development and efficacy of a novel mRNA cocktail for the delivery of African swine fever virus antigens and induction of immune responses., PMID:40298440

Safety and efficacy of a Vero-adapted live attenuated vaccine candidate for African swine fever., PMID:40279925

A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins., PMID:40266072

Characterization of a swine-derived single-chain fragment variable targeting the conserved immunodominant epitope of African swine fever virus p30., PMID:40254197

Lipid nanoparticle-encapsulated DNA vaccine encoding African swine fever virus p54 antigen elicits robust immune responses in pigs., PMID:40250107

Unraveling the immunogenic landscape: A quest for B-cell linear epitopes in the African swine fever virus H339R protein., PMID:40210029

Epitope mapping targeting the K205R protein of African swine fever virus using nanobody as a novel tool., PMID:40172217

Development and application of a blocking ELISA method based on Cap protein for detecting antibodies against porcine circovirus 2., PMID:40162771

Screening of aptamer for p30 protein of African swine fever virus and establishment of electrochemical immunosensor for detection of p30 antibody., PMID:40154044

Preparation of a new monoclonal antibody against D205R protein of African swine fever virus and identification of its linear antigenic epitope., PMID:40112994

Computational insights in CD58 binding to ASFV CD2v and in silico optimization of nanobody designs against the interface., PMID:40092189

Rapid detection of African swine fever virus by a blue latex microsphere immunochromatographic strip., PMID:40088339

A Mutant of Africa Swine Fever Virus Protein p72 Enhances Antibody Production and Regulates the Production of Cytokines., PMID:40006949

Monoclonal Antibodies Targeting Porcine Macrophages Are Able to Inhibit the Cell Entry of Macrophage-Tropic Viruses (PRRSV and ASFV)., PMID:40006922

Evaluation of the Immunogenicity of a Pool of Recombinant Lactococcus lactis Expressing Eight Antigens of African Swine Fever Virus in a Mouse Model., PMID:40005900

African Swine Fever Vaccine Candidate ASFV-G-ΔI177L/ΔLVR Protects Against Homologous Virulent Challenge and Exhibits Long-Term Maintenance of Antibodies., PMID:40002955

The capsid protein p72 specific mAb and the corresponding novel epitope based ELISAs for detection of ASFV infection., PMID:39999573

An attenuated African swine fever virus expressing the E2 glycoprotein of classical swine fever virus protects pigs against challenge of both viruses., PMID:39964030

Development and Evaluation of an Immunochromatographic Strip and a Magnetic Chemiluminescence Immunoassay for Detection of Porcine Circovirus Type 2 Antigen., PMID:39852915

A Pool of Bacterium-like Particles Displaying African Swine Fever Virus Antigens Induces Both Humoral and Cellular Immune Responses in Pigs., PMID:39852784

p54-Fc-Labeled Gold Nanoparticle-Based Lateral Flow Strip-Assisted Portable Devices for Rapid and Quantitative Point-of-Care Detection of ASFV Antibodies., PMID:39852075

Ferritin nanoparticles significantly enhance the immune response to the African swine fever virus p34 protein., PMID:39826780

Design of a Multiepitope Pan-Proteomic mRNA Vaccine Construct Against African Swine Fever Virus: A Reverse Vaccinology Approach., PMID:39803351

A PDMS/chitosan/MPMs composite film based on multi-field coupling enhancement for African swine fever virus P72 protein detection., PMID:39799276

Development of monoclonal antibodies for ASFV K205R protein and precise mapping of linear antigenic epitopes., PMID:39793793

Development of a Synthetic VP1 Protein Peptide-Based ELISA to Detect Antibodies Against Porcine Bocavirus Group 3., PMID:39772251

Double Deletion of EP402R and EP153R in the Attenuated Lv17/WB/Rie1 African Swine Fever Virus (ASFV) Enhances Safety, Provides DIVA Compatibility, and Confers Complete Protection Against a Genotype II Virulent Strain., PMID:39772067

Newcastle Disease Virus-Vectored African Swine Fever Virus Antigen Cocktail Delays the Onset of ASFV-SY18 but Is Not Protective., PMID:39770792

Identification of a New Conserved Antigenic Epitope by Specific Monoclonal Antibodies Targeting the African Swine Fever Virus Capsid Protein p17., PMID:39728990

A triple protein-based ELISA for differential detection of ASFV antibodies., PMID:39723184

[Preparation and immunogenicity evaluation of ferritin nanoparticles conjugated with African swine fever virus p30 protein]., PMID:39722512

From structure prediction to function: defining the domain on the African swine fever virus CD2v protein required for binding to erythrocytes., PMID:39688401

Serologic differentiation between wild-type and cell-adapted African swine fever virus infections: A novel DIVA strategy using the MGF100-1L protein., PMID:39675188

Detection of African swine fever virus antibodies using p11.5 and p14.5 protein-based indirect ELISA., PMID:39673968

Recombinant African swine fever virus p30-flagellin fusion protein promotes p30-specific humoral and cellular immune responses in mice., PMID:39671731

Development and Validation of an Indirect and Blocking ELISA for the Serological Diagnosis of African Swine Fever., PMID:39599534

Development and characterization of high-efficiency cell-adapted live attenuated vaccine candidate against African swine fever., PMID:39584308

Identification of linear B cell epitopes on the E146L protein of African swine fever virus with monoclonal antibodies., PMID:39533386

A Nanobody-based TRIM-away targets the intracellular protein degradation of African swine fever virus., PMID:39488886

ASFV subunit vaccines: Strategies and prospects for future development., PMID:39442810

Identification and characterization of linear B-cell epitopes on African swine fever virus H171R protein., PMID:39440988

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-ASFV pM1249L Polyclonal Antibody [PVV38701]
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