Catalog No.
PHF07601
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human SLC12A2 (Asn1002-Ser1212).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, SLC12A2, NKCC1
Purification
Purified by antigen affinity column.
Accession
P55011
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Expression patterns of NKCC1 in neurons and non-neuronal cells during cortico-hippocampal development., PMID:36573432
NKCC1, an Elusive Molecular Target in Brain Development: Making Sense of the Existing Data., PMID:33291778
Staurosporine and NEM mainly impair WNK-SPAK/OSR1 mediated phosphorylation of KCC2 and NKCC1., PMID:32413057
Epithelial disruptions, but not immune cell invasion, induced secretory dysfunction following innate immune activation in a novel model of acute salivary gland injury., PMID:29160910
Depolarizing γ-aminobutyric acid contributes to glutamatergic network rewiring in epilepsy., PMID:28074534
Effect of vasopressin on Na(+)-K(+)-2Cl(-) cotransporter (NKCC) and the signaling mechanisms on the murine late distal colon., PMID:26656758
Targeted expression of anoctamin calcium-activated chloride channels in rod photoreceptor terminals of the rodent retina., PMID:23557741
Regulation of brain tumor dispersal by NKCC1 through a novel role in focal adhesion regulation., PMID:22570591
Molecular and functional expression of cation-chloride cotransporters in dorsal root ganglion neurons during postnatal maturation., PMID:22457464
Cellular distribution of NKCC2 in the gastric mucosa and its response to short-term osmotic shock., PMID:22388656
Forkhead transcription factor FoxA1 regulates sweat secretion through Bestrophin 2 anion channel and Na-K-Cl cotransporter 1., PMID:22223659
An autocrine neuronal interleukin-6 loop mediates chloride accumulation and NKCC1 phosphorylation in axotomized sensory neurons., PMID:21940443
Role of NKCC in BK channel-mediated net K⁺ secretion in the CCD., PMID:21816753
Phosphorylation and transport in the Na-K-2Cl cotransporters, NKCC1 and NKCC2A, compared in HEK-293 cells., PMID:21464992
Expression of the sodium potassium chloride cotransporter (NKCC1) and sodium chloride cotransporter (NCC) and their effects on rat lens transparency., PMID:20458365
Chloride Is essential for capacitation and for the capacitation-associated increase in tyrosine phosphorylation., PMID:18957426
Expression of aquaporin1, 3, and 4, NKCC1, and NKCC2 in the human endolymphatic sac., PMID:18606512
Spatial and temporal distribution patterns of Na-K-2Cl cotransporter in adult and developing mouse retinas., PMID:18442435
Roles of basolateral solute uptake via NKCC1 and of myosin II in vasopressin-induced cell swelling in inner medullary collecting duct., PMID:18417545
NKCC1 phosphorylation stimulates neurite growth of injured adult sensory neurons., PMID:17581962
SLC26A7 can function as a chloride-loading mechanism in parietal cells., PMID:17404755
Protein expression and mRNA cellular distribution of the NKCC1 cotransporter in the dorsal root and trigeminal ganglia of the rat., PMID:16904086
Gene expression in rat lacrimal gland duct cells collected using laser capture microdissection: evidence for K+ secretion by duct cells., PMID:16638994
Novel insights regarding the operational characteristics and teleological purpose of the renal Na+-K+-Cl2 cotransporter (NKCC2s) splice variants., PMID:16157691
Bumetanide, the specific inhibitor of Na+-K+-2Cl- cotransport, inhibits 1alpha,25-dihydroxyvitamin D3-induced osteoclastogenesis in a mouse co-culture system., PMID:12955156
GABA-mediated trophic effect on oligodendrocytes requires Na-K-2Cl cotransport activity., PMID:12904508
Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD., PMID:12851251
Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1)., PMID:12386165
Activation of the Na-K-Cl cotransporter NKCC1 detected with a phospho-specific antibody., PMID:12145305
Intrinsic optical signals in the rat optic nerve: role for K(+) uptake via NKCC1 and swelling of astrocytes., PMID:11754210
Expression of the Na+K+-2CI- cotransporter in alpha and beta cells isolated from the rat pancreas., PMID:11510890
For research use only. Not for human or drug use.
