Catalog No.
PHD47501
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human ZP3 (Thr171-Gly363).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Zona pellucida sperm-binding protein 3, Sperm receptor, ZP3A/ZP3B, Zona pellucida glycoprotein 3, Zp-3, Zona pellucida protein C, Processed zona pellucida sperm-binding protein 3, ZP3, ZP3A, ZP3B, ZPC
Purification
Purified by antigen affinity column.
Accession
P21754
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Generation and Characterization of a TRIM21 Overexpressing Mouse Line., PMID:39487578
Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice., PMID:36752682
Isolation of female germline stem cells from neonatal piglet ovarian tissue and differentiation into oocyte-like cells., PMID:36525858
Initial detachment of the mouse oocyte from the zona pellucida is mediated by metallopeptidase activity†., PMID:36179245
Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice., PMID:35252419
Contraceptive efficacy of recombinant porcine zona proteins and fusion protein encompassing canine ZP3 fragment and GnRH in female beagle dogs., PMID:35249251
Immunocontraceptive potential of a GnRH receptor-based fusion recombinant protein., PMID:33945047
Adoptive transfers of CD4+ CD25+ Tregs partially alleviate mouse premature ovarian insufficiency., PMID:32741069
Researching immunocontraceptive vaccines with mares (Equus caballus) as both a target and model for African elephant (Loxodonta africana) cows: A review., PMID:31208844
The application of naked DNA plasmid (DrZP3) and recombinant adenovirus (Ad-rZP3) in rat animal model to determine comparative efficacy of ZP3-Immunocontraceptive vaccines., PMID:31163251
Serological and histological evaluation of species-specific immunocontraceptive vaccine antigens based on zona pellucida 3 in the small Indian mongoose (Herpestes auropunctatus)., PMID:30587673
Efficient production of human zona pellucida-3 in a prokaryotic expression system., PMID:30341457
The Human Egg's Zona Pellucida., PMID:29853184
The preclinical evaluation of immunocontraceptive vaccines based on canine zona pellucida 3 (cZP3) in a mouse model., PMID:29751768
Effect of human ZP3 monoclonal antibody on expression of GDF-9 and number of theca cells in ovary of mice (Mus musculus)., PMID:31435327
Inhibition of PSMD4 alters ZP1 ubiquitination state and sperm-oocyte-binding ability in pigs., PMID:29575084
Protective function of Bu Shen Huo Xue formula on the immunity of B6AF1 mice with experimental autoimmune premature ovarian failure., PMID:29545848
Enhanced contraception of canine zona pellucida 3 DNA vaccine via targeting DEC-205 in mice., PMID:29455062
Effects of hPMSCs on granulosa cell apoptosis and AMH expression and their role in the restoration of ovary function in premature ovarian failure mice., PMID:29386068
The roles of protein disulphide isomerase family A, member 3 (ERp57) and surface thiol/disulphide exchange in human spermatozoa-zona pellucida binding., PMID:28175305
Egg Envelope Glycoproteins ZP1 and ZP3 Mediate Sperm-Egg Interaction in the Japanese Quail., PMID:32908412
Contraceptive efficacy of recombinant fusion protein comprising zona pellucida glycoprotein-3 fragment and gonadotropin releasing hormone., PMID:26859695
Ovarian function and pregnancy outcome in pony mares following immunocontraception with native and recombinant porcine zona pellucida vaccines., PMID:26708619
[Expression of Ki-67 and estrogen receptor in the uterus of mice with autoimmune premature ovarian failure induced by peptide zona pellucida 3]., PMID:26198948
Amyloid properties of the mouse egg zona pellucida., PMID:26043223
Isolation and enrichment of mouse female germ line stem cells., PMID:25685731
Role of focal adhesion kinase in oocyte-follicle communication., PMID:25536210
Canine zona pellucida glycoprotein-3: up-scaled production, immunization strategy and its outcome on fertility., PMID:25448113
Role of zona pellucida glycoproteins during fertilization in humans., PMID:25445843
Moonlighting proteins in sperm-egg interactions., PMID:25399599
Proteomic Characterization of Pig Sperm Anterior Head Plasma Membrane Reveals Roles of Acrosomal Proteins in ZP3 Binding., PMID:25078272
The unique neonatal NK cells: a critical component required for neonatal autoimmune disease induction by maternal autoantibody., PMID:24904590
ORMDL3 transgenic mice have increased airway remodeling and airway responsiveness characteristic of asthma., PMID:24623133
Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3., PMID:24334245
Evaluation of recombinant fusion protein comprising dog zona pellucida glycoprotein-3 and Izumo and individual fragments as immunogens for contraception., PMID:24333343
Expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of ZP3 protein in oocytes in a natural, wild triploid crucian carp mutant, Carassius auratus var. Pingxiangnensis., PMID:24301933
Mass spectrometry investigation of glycosylation on the NXS/T sites in recombinant glycoproteins., PMID:23632316
Immunogenicity and contraceptive efficacy of Escherichia coli-expressed recombinant porcine zona pellucida proteins., PMID:23444974
Identification of sperm head proteins involved in zona pellucida binding., PMID:23355646
Production of tag-free recombinant fusion protein encompassing promiscuous T cell epitope of tetanus toxoid and dog zona pellucida glycoprotein-3 for contraceptive vaccine development., PMID:23242635
The effects of GM-CSF and IL-5 as molecular adjuvants on immune responses and contraception induced by mZP3 DNA vaccination., PMID:22934542
Genetic and physiological study of morphologically abnormal human zona pellucida., PMID:22889493
Sperm proteasome degrades egg envelope glycoprotein ZP1 during fertilization of Japanese quail (Coturnix japonica)., PMID:22859519
Reversine promotes porcine muscle derived stem cells (PMDSCs) differentiation into female germ-like cells., PMID:22821411
Diverse lectin-binding specificity of four ZP3 glycoprotein isoforms with a discrete isoelectric point in chicken egg coat., PMID:22776203
Mapping of minimal motifs of B-cell epitopes on human zona pellucida glycoprotein-3., PMID:22162720
Porcine zona pellucida glycoprotein ZP4 is responsible for the sperm-binding activity of the ZP3/ZP4 complex., PMID:22008510
A novel treatment strategy for ovarian cancer based on immunization against zona pellucida protein (ZP) 3., PMID:21974931
Oocytic expression of zona pellucida protein ZP4 in Japanese quail (Coturnix japonica)., PMID:21729200
Evidence for the inhibition of fertilization in vitro by anti-ZP3 antisera derived from DNA vaccine., PMID:21596079
For research use only. Not for human or drug use.
