Catalog No.
PHD01701
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PRKAR2A (Glu266-Gln404).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A, PKR2, PRKAR2
Purification
Purified by antigen affinity column.
Accession
P13861
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Hypoxia and the hypoxia inducible factor 1α activate protein kinase A by repressing RII beta subunit transcription., PMID:32111982
PKA-RII subunit phosphorylation precedes activation by cAMP and regulates activity termination., PMID:29615473
Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies., PMID:25926605
Regulation of rat alveolar type 2 cell proliferation in vitro involves type II cAMP-dependent protein kinase., PMID:16980378
An active protein kinase A (PKA) is involved in meiotic arrest of rat growing oocytes., PMID:16816331
The role of protein kinase A anchoring via the RII alpha regulatory subunit in the murine immune system., PMID:15905526
Identification and characterization of novel PKA holoenzymes in human T lymphocytes., PMID:15794744
Cyclic AMP-receptor proteins in human salivary glands., PMID:14566599
Human myometrial quiescence and activation during gestation and parturition involve dramatic changes in expression and activity of particulate type II (RII alpha) protein kinase A holoenzyme., PMID:12727975
Evidence for involvement of A-kinase anchoring protein in activation of rat arterial K(ATP) channels by protein kinase A., PMID:11600677
Positive regulation of cell-cell and cell-substrate adhesion by protein kinase A., PMID:11591815
Localization of cAMP-dependent signal transducers in early rat liver carcinogenesis., PMID:9541468
Cyclic AMP-dependent protein kinase (cAK) in human B cells: co-localization of type I cAK (RI alpha 2 C2) with the antigen receptor during anti-immunoglobulin-induced B cell activation., PMID:8647207
The differential response of protein kinase A to cyclic AMP in discrete brain areas correlates with the abundance of regulatory subunit II., PMID:8627334
Cloning and characterization of A-kinase anchor protein 100 (AKAP100). A protein that targets A-kinase to the sarcoplasmic reticulum., PMID:7721854
Post-translational abnormality of the type II cyclic AMP-dependent protein kinase in psoriasis: modulation by retinoic acid., PMID:7615649
Differential localization of two isoforms of the regulatory subunit RII alpha of cAMP-dependent protein kinase in human sperm: biochemical and cytochemical study., PMID:7893490
Protein kinase A (PK-A) regulatory subunit expression in colorectal cancer and related mucosa., PMID:8142263
Cloning and characterization of AKAP 95, a nuclear protein that associates with the regulatory subunit of type II cAMP-dependent protein kinase., PMID:8125992
Characterization of in-vitro-translated human regulatory and catalytic subunits of cAMP-dependent protein kinases., PMID:8119290
Cellular location and age-dependent changes of the regulatory subunits of cAMP-dependent protein kinase in rat testis., PMID:8107013
For research use only. Not for human or drug use.
