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Anti-Human MYF5 Polyclonal Antibody

Catalog #:   PHC97101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P13349
Overview

Catalog No.

PHC97101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MYF5 (Cys72-Ser147).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, bHLHc2, MYF5, BHLHC2

Purification

Purified by antigen affinity column.

Accession

P13349

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MYF5 antibody (PHC97101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
References

The Satellite Cell at 60: The Foundation Years., PMID:34459412

Establishment of primary chicken embryo myoblast cell culture, antigenic epitopes prediction and production of anti activin receptor type IIB polyclonal antibody in chicken., PMID:33491546

Deficient muscle regeneration potential in sarcopenic COPD patients: Role of satellite cells., PMID:32989805

Toxoplasma gondii Impairs Myogenesis in vitro, With Changes in Myogenic Regulatory Factors, Altered Host Cell Proliferation and Secretory Profile., PMID:31828046

mTORC2 affects the maintenance of the muscle stem cell pool., PMID:31791403

Primary myogenesis in the sand lizard (Lacerta agilis) limb bud., PMID:31214772

Chick embryonic cells as a source for generating in vitro model of muscle cell dystrophy., PMID:30302616

Influence of static magnetic fields on human myoblast/mesenchymal stem cell co‑cultures., PMID:29286120

Trichinella spiralis muscle larvae excretory-secretory products induce changes in cytoskeletal and myogenic transcription factors in primary myoblast cultures., PMID:29258830

Nutrient sensing signaling integrates nutrient metabolism and intestinal immunity in grass carp, Ctenopharyngodon idellus after prolonged starvation., PMID:28964867

p38 MAPK activation and H3K4 trimethylation is decreased by lactate in vitro and high intensity resistance training in human skeletal muscle., PMID:28467493

Selective androgen receptor modulator, YK11, regulates myogenic differentiation of C2C12 myoblasts by follistatin expression., PMID:23995658

SPARC is up-regulated during skeletal muscle regeneration and inhibits myoblast differentiation., PMID:23670848

Sorting single satellite cells from individual myofibers reveals heterogeneity in cell-surface markers and myogenic capacity., PMID:23407661

The obestatin/GPR39 system is up-regulated by muscle injury and functions as an autocrine regenerative system., PMID:22992743

Impact of static magnetic fields on human myoblast cell cultures., PMID:21837362

Alpha 6 integrin is important for myogenic stem cell differentiation., PMID:21763619

Genetic polymorphisms of the enzymes involved in DNA methylation and synthesis in elite athletes., PMID:21673074

Emerin inhibits Lmo7 binding to the Pax3 and MyoD promoters and expression of myoblast proliferation genes., PMID:21525034

Hes6 is required for actin cytoskeletal organization in differentiating C2C12 myoblasts., PMID:21501606

Polycomb EZH2 controls self-renewal and safeguards the transcriptional identity of skeletal muscle stem cells., PMID:21498568

Characterization of human myoblast differentiation for tissue-engineering purposes by quantitative gene expression analysis., PMID:21370490

Evidence of heterogeneity within bovine satellite cells isolated from young and adult animals., PMID:21297061

Role of endogenous TGF-β family in myogenic differentiation of C2C12 cells., PMID:21268083

Accumulation of β-catenin by lithium chloride in porcine myoblast cultures accelerates cell differentiation., PMID:20857211

Pax3 induces differentiation of juvenile skeletal muscle stem cells without transcriptional upregulation of canonical myogenic regulatory factors., PMID:20605921

Sulf1A and HGF regulate satellite-cell growth., PMID:20442248

Production of a rat monoclonal antibody specific for Myf5., PMID:20199153

Satellite cell characterization from aging human muscle., PMID:20092696

The depletion of skeletal muscle satellite cells with age is concomitant with reduced capacity of single progenitors to produce reserve progeny., PMID:20079729

Expression and subcellular localization of myogenic regulatory factors during the differentiation of skeletal muscle C2C12 myoblasts., PMID:19830700

DUX4c, an FSHD candidate gene, interferes with myogenic regulators and abolishes myoblast differentiation., PMID:18723017

Trophism of neural progenitor cells to embryonic stem cells: neural induction and transplantation in a mouse ischemic stroke model., PMID:17492787

MyoD-positive epiblast cells regulate skeletal muscle differentiation in the embryo., PMID:17060497

Extraocular muscle morphogenesis and gene expression are regulated by Pitx2 gene dose., PMID:16638982

Muscle-derived stem cells used to treat skin defects prevent wound contraction and expedite reepithelialization., PMID:16630112

Integrin alpha6beta1-laminin interactions regulate early myotome formation in the mouse embryo., PMID:16554364

A 23-amino acid motif spanning the basic domain targets zebrafish myogenic regulatory factor myf5 into nucleolus., PMID:16225396

The role of neurotrophins in the maintenance of the spinal cord motor neurons and the dorsal root ganglia proprioceptive sensory neurons., PMID:16183241

Muscle-derived stem cells in tissue engineering: defining cell properties suitable for construct design., PMID:15944940

Rapid evolution of muscle fibre number in post-glacial populations of Arctic charr Salvelinus alpinus., PMID:15557021

Normal and aberrant craniofacial myogenesis by grafted trunk somitic and segmental plate mesoderm., PMID:15269174

Skeletal muscle atrophy is associated with an increased expression of myostatin and impaired satellite cell function in the portacaval anastamosis rat., PMID:15256363

Ste20-like kinase SLK displays myofiber type specificity and is involved in C2C12 myoblast differentiation., PMID:15052621

TGF-beta 1 signaling controls retinal pericyte contractile protein expression., PMID:14609524

FGF-2 antagonizes the TGF-beta1-mediated induction of pericyte alpha-smooth muscle actin expression: a role for myf-5 and Smad-mediated signaling pathways., PMID:14578427

Immunophenotype of desmoplastic small round cell tumors as detected in cases with EWS-WT1 gene fusion product., PMID:12640103

Expression of basic helix-loop-helix proteins in the glomeruli., PMID:11200868

Transdifferentiation of esophageal smooth to skeletal muscle is myogenic bHLH factor-dependent., PMID:10725239

The growth of the dermomyotome and formation of early myotome lineages in thoracolumbar somites of chicken embryos., PMID:10648247

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human MYF5 Polyclonal Antibody [PHC97101]
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