Catalog No.
PHC23501
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CENPB (Ser181-Gly392).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB
Purification
Purified by antigen affinity column.
Accession
P07199
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Should We Adopt Increased Dilutions for Indirect Immunofluorescence in Pediatric Anti-Centromere Antibody Testing? Insights from a Three-Year Retrospective Study., PMID:39857867
Autoimmune diseases, autoantibody status and silicosis in a cohort of 1238 workers from the artificial stone benchtop industry., PMID:39134395
Coexistence of systemic sclerosis and cryopyrin-associated periodic syndrome., PMID:38940218
Neuronal autoantibodies in the cerebrospinal fluid of 148 patients with schizophrenia and 151 healthy controls., PMID:38770306
Detection of IgE-autoantibodies to nuclear antigens in patients with systemic sclerosis and analysis of their clinical relevance., PMID:38530662
[Clinical and immunological features of primary Sjögren's syndrome patients with positive anti-centromere protein B antibody]., PMID:38101794
The Role of Indirect Immunofluorescence (IIF) and Line Immunoassay (LIA) in the Diagnosis of Autoimmune Diseases and Their Clinical Correlation: An Observational Study From a Tertiary Care Center in Bihar., PMID:38022160
Prevalence and Clinical Implications of Scleroderma-Specific Autoantibodies in Seronegative Patients with Sicca Complaints., PMID:37941855
Clinical value of chemiluminescence method for detection of antinuclear antibody profiles., PMID:37901001
Subclinical coronary atherosclerosis, detected by computer tomography with coronary calcium score, and the occurrence of major cardiovascular events at 5 years of follow-up in a cohort of patients with systemic sclerosis., PMID:37316356
Autoantibodies and Clinical Correlations in Polish Systemic Sclerosis Patients: A Cross-Sectional Study., PMID:36675584
Autoantibodies associated with systemic sclerosis in three autoimmune diseases imprinted by type I interferon gene dysregulation: a comparison across SLE, primary Sjögren's syndrome and systemic sclerosis., PMID:36581379
Feasibility of Indirect immunofluorescence (IIF) alone as a screening method for antinuclear antibody in connective diseases in India's sub-Himalayan region., PMID:36308197
Seropositive Neuromyelitis Optica in a Case of Undiagnosed Ankylosing Spondylitis: A Neuro-Rheumatological Conundrum., PMID:35864917
Analytical and clinical performance of different platforms simultaneously detecting 15 antinuclear antibodies., PMID:35708068
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays., PMID:35690095
Serum Biomarker Panel for Rapid Early Diagnosis of Lung Cancer., PMID:35616661
[Investigation of the Contribution of Autoantibodies to Clinical Diagnosis in Liver Pathologies and the Identification of Accompanying Autoimmune Diseases]., PMID:35088962
Systemic Sclerosis in Zimbabwe: Autoantibody Biomarkers, Clinical, and Laboratory Correlates., PMID:34858387
Increased Autoantibodies Against Ro/SS-A, CENP-B, and La/SS-B in Patients With Kidney Allograft Antibody-mediated Rejection., PMID:34557585
Anticentromere antibody induced by immunization with centromere protein and Freund's complete adjuvant may interfere with mouse early-stage embryo., PMID:34416895
Comparison of antinuclear antibody profiles obtained using line immunoassay and fluorescence enzyme immunoassay., PMID:34154430
Combining multi-antigenic immunodot with indirect immunofluorescence on HEp-2 cells improves the diagnosis of systemic sclerosis., PMID:34111525
Autoantibody profiles in systemic sclerosis; a comparison of diagnostic tests., PMID:33818234
[Significance of anti-tubulin-α-1C autoantibody in systemic sclerosis]., PMID:33331306
Anti-centromere antibodies target centromere-kinetochore macrocomplex: a comprehensive autoantigen profiling., PMID:33208344
Understanding and interpreting antinuclear antibody tests in systemic rheumatic diseases., PMID:33154583
Detection of Autoantibodies in Bronchoalveolar Lavage in Patients with Diffuse Interstitial Lung Disease., PMID:33069461
Coexistence of systemic and localized scleroderma: a systematic literature review and observational cohort study., PMID:32653905
Sporadic late-onset nemaline myopathy in a patient with silicone breast implants., PMID:32590250
Proteomics characterization of CENP-B epitope in Moroccan scleroderma patients with anti-centromere autoantibodies., PMID:32057908
Multiparametric Analysis of Connective Tissue Disease Specific Autoantibodies Using a Spot Immunoassay., PMID:31532087
Clinical and laboratory features of African-Brazilian patients with systemic sclerosis., PMID:31065858
Reproducibility of fluid-phase measurements in PBS-treated sputum supernatant of healthy and stable COPD subjects., PMID:31043775
Evaluation of a commercial immunoassay for autoantibodies in Chinese Han systemic sclerosis population., PMID:30685360
Bicaudal D2 is a novel autoantibody target in systemic sclerosis that shares a key epitope with CENP-A but has a distinct clinical phenotype., PMID:29369808
Autoantibodies of IgM and IgG classes show differences in recognition of multiple autoantigens in chronic obstructive pulmonary disease., PMID:28951328
Disease-related autoantibody profile in patients with systemic sclerosis., PMID:28749191
Elucidating Protein-DNA Interactions in Human Alphoid Chromatin via Hybridization Capture and Mass Spectrometry., PMID:28704058
Clinical evaluation of CENP-B and Scl-70 autoantibodies in silicosis patients., PMID:28587321
Microinjection of Antibodies Targeting the Lamin A/C Histone-Binding Site Blocks Mitotic Entry and Reveals Separate Chromatin Interactions with HP1, CenpB and PML., PMID:28346356
Relevance of clinical and autoantibody profiles in systemic sclerosis among Thais., PMID:28296274
Increased immunoreactivity against human cytomegalovirus UL83 in systemic sclerosis., PMID:28240591
Clinical autoantibody detection by microarray., PMID:27658157
Anticentromere antibody-positive primary Sjögren's syndrome: Epitope analysis of a subset of anticentromere antibody-positive patients., PMID:27161330
Autoantibody-Positive Healthy Individuals Display Unique Immune Profiles That May Regulate Autoimmunity., PMID:27059145
Interpretation of an Extended Autoantibody Profile in a Well-Characterized Australian Systemic Sclerosis (Scleroderma) Cohort Using Principal Components Analysis., PMID:26246178
A cross-sectional study of autoantibody profiles in the Waikato systemic sclerosis cohort, New Zealand., PMID:26022620
Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing., PMID:26020561
Diversity of humoral responses to the centromere proteins among HCV-related chronic liver disease, PBC and AIH patients., PMID:25220385
For research use only. Not for human or drug use.
