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Anti-Human PRPF4 Polyclonal Antibody

Catalog #:   PHA59301 Specific References (4) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O43172
Overview

Catalog No.

PHA59301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PRPF4 (Ser220-Glu522).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

U4/U6 small nuclear ribonucleoprotein Prp4, PRP4 homolog, hPrp4, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4, PRPF4, PRP4

Purification

Purified by antigen affinity column.

Accession

O43172

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PRPF4 antibody (PHA59301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 36 kDa
    Observed MW: 36 kDa
References

PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores., PMID:17998396

Central region of the human splicing factor Hprp3p interacts with Hprp4p., PMID:11971898

Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome., PMID:9328476

The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p., PMID:9257651

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human PRPF4 Polyclonal Antibody [PHA59301]
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