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Anti-Human RTCA Polyclonal Antibody

Catalog #:   PHA11601 Specific References (40) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O00442
Overview

Catalog No.

PHA11601

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RTCA (Met1-Leu366).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

RNA 3'-terminal phosphate cyclase, RNA cyclase, RNA-3'-phosphate cyclase, 6.5.1.4, RNA terminal phosphate cyclase domain-containing protein 1, RTC domain-containing protein 1, RTCA, RPC, RPC1, RTC1, RTCD1

Purification

Purified by antigen affinity column.

Accession

O00442

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RTCA antibody (PHA11601) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 42 kDa
    Observed MW: 42 kDa
References

Combined toxicity evaluation of polystyrene nanoplastics and Nano-ZnO of distinctive morphology on human lung epithelial cells., PMID:40112547

Evaluation of three alternative methods to the plaque reduction neutralizing assay for measuring neutralizing antibodies to dengue virus serotype 2., PMID:39227969

Targeting GD2 after allogeneic SCT: effector cell composition defines the optimal use of ch14.18 and the bispecific antibody construct NG-CU (GD2-CD3)., PMID:37742286

Effects of natural 24-epibrassinolide on inducing apoptosis and restricting metabolism in hepatocarcinoma cells., PMID:36115171

Real-time cell analysis: A high-throughput approach for testing SARS-CoV-2 antibody neutralization and escape., PMID:35578733

Anti-PD1 antibody enhances the anti-tumor efficacy of MUC1-MBP fusion protein vaccine via increasing Th1, Tc1 activity and decreasing the proportion of MDSC in the B16-MUC1 melanoma mouse model., PMID:34607233

A Promising, Novel Radiosensitizer Nanodrug Complex for Oral Cavity Cancer: Cetuximab and Cisplatin-Conjugated Gold Nanoparticles., PMID:34462254

A new immunotherapy strategy targeted CD30 in peripheral T-cell lymphomas: CAR-modified T-cell therapy based on CD30 mAb., PMID:33514882

The Effect of Different Immunization Cycles of a Recombinant Mucin1-Maltose-Binding Protein Vaccine on T Cell Responses to B16-MUC1 Melanoma in Mice., PMID:32823603

Construction of Genomic Library and High-Throughput Screening of Pseudomonas aeruginosa Novel Antigens for Potential Vaccines., PMID:32779581

Cetuximab-induced natural killer cell cytotoxicity in head and neck squamous cell carcinoma cell lines: investigation of the role of cetuximab sensitivity and HPV status., PMID:32541873

PLAP -CAR T cells mediate high specific cytotoxicity against colon cancer cells., PMID:32472757

Enhanced activation of human NK cells by drug-exposed hepatocytes., PMID:32060585

Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro., PMID:31948561

Analysis of Transcriptome, Selected Intracellular Signaling Pathways, Proliferation and Apoptosis of LNCaP Cells Exposed to High Leptin Concentrations., PMID:31671654

R2-8018 reduces the proliferation and migration of non-small cell lung cancer cells by disturbing transactivation between M3R and EGFR., PMID:31401315

TREM1/Dap12-based CAR-T cells show potent antitumor activity., PMID:31268375

Data on migration of the non-invasive breast cancer cell line, MCF-7 treated with Bevacizumab using Real Time Cell Analyzer (RTCA)., PMID:30671510

Calcium phosphate altered the cytokine secretion of macrophages and influenced the homing of mesenchymal stem cells., PMID:32254303

Real-time analysis of the cytopathic effect of African swine fever virus., PMID:29627336

Calcium Mobilization and Inhibition of Akt Reduced the Binding of PEO-1 Cells to Fibronectin., PMID:32454640

Impedance-based analysis of Natural Killer cell stimulation., PMID:29563530

In vitro immunotherapy potency assays using real-time cell analysis., PMID:29499048

BAG3 promotes the phenotypic transformation of primary rat vascular smooth muscle cells via TRAIL., PMID:29484366

TRAIL DR5-CTSB crosstalk participates in breast cancer autophagy initiated by SAHA., PMID:29018571

Identification of a specific agonist of human TAS2R14 from Radix Bupleuri through virtual screening, functional evaluation and binding studies., PMID:28939897

Endothelial integrity may be regulated by a specific antigen via an IgE-mediated mechanism., PMID:28258676

Down-Regulation of RNA 3'-Terminal Phosphate Cyclase Attenuates Toll-Like Receptor 3-Mediated Axonal Loss in the Retina and Optic Nerve., PMID:27727398

Monitoring and functional characterization of the lymphocytic compartment in pancreatic ductal adenocarcinoma patients., PMID:27424476

Multidimensional GPCR profiling and screening using impedance-based label-free and real-time assay., PMID:25563187

Examining the protective role of ErbB2 modulation in human-induced pluripotent stem cell-derived cardiomyocytes., PMID:25055963

[Research on human enterovirus 71 infectivity assay based on a real-time cell analysis]., PMID:23895003

Real-time cell analysis--a new method for dynamic, quantitative measurement of infectious viruses and antiserum neutralizing activity., PMID:23835032

Real-time monitoring of hematopoietic cell interaction with fibronectin fragment: the effect of histone deacetylase inhibitors., PMID:23567296

Efficacy of cetuximab and panitumumab in oral squamous cell carcinoma cell lines: prognostic value of MAGE-A subgroups for treatment success., PMID:23375534

Assessment of in vitro heparin complement regulation capacity during real-time cell analyzer antibody-mediated cytolysis assay: compatibility studies for pig-to-baboon xenotransplantation., PMID:22841220

Novel, real-time cell analysis for measuring viral cytopathogenesis and the efficacy of neutralizing antibodies to the 2009 influenza A (H1N1) virus., PMID:22363775

Responses after one dose of a monovalent influenza A (H1N1) 2009 inactivated vaccine in Chinese population--a practical observation., PMID:21767595

Real-time monitoring of flavivirus induced cytopathogenesis using cell electric impedance technology., PMID:21349291

Validation of xCELLigence real-time cell analyzer to assess compatibility in xenotransplantation with pig-to-baboon model., PMID:20970663

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human RTCA Polyclonal Antibody [PHA11601]
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